دورية أكاديمية
Longitudinal survey of Clostridium difficile presence and gut microbiota composition in a Belgian nursing home
| العنوان: | Longitudinal survey of Clostridium difficile presence and gut microbiota composition in a Belgian nursing home |
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| المؤلفون: | Rodriguez Diaz, Cristina, Taminiau, Bernard, Korsak Koulagenko, Nicolas, Avesani, Véronique, Van Broeck, Johan, Brach, Philippe, Delmée, Michel, Daube, Georges |
| المصدر: | BMC Microbiology, 16 (229) (2016) |
| بيانات النشر: | BioMed Central |
| سنة النشر: | 2016 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | C. difficile, Elderly care home residents, 16S rRNA gene analysis, Human health sciences, Gastroenterology & hepatology, Sciences de la santé humaine, Gastroentérologie & hépatologie |
| الوصف: | peer reviewed ; ackground Increasing age, several co-morbidities, environmental contamination, antibiotic exposure and other intestinal perturbations appear to be the greatest risk factors for C. difficile infection (CDI). Therefore, elderly care home residents are considered particularly vulnerable to the infection. The main objective of this study was to evaluate and follow the prevalence of C. difficile in 23 elderly care home residents weekly during a 4-month period. A C. difficile microbiological detection scheme was performed along with an overall microbial biodiversity study of the faeces content by 16S rRNA gene analysis. Results Seven out of 23 (30.4 %) residents were (at least one week) positive for C. difficile. C. difficile was detected in 14 out of 30 diarrhoeal samples (43.7 %). The most common PCR-ribotype identified was 027. MLVA showed that there was a clonal dissemination of C. difficile strains within the nursing home residents. 16S-profiling analyses revealed that each resident has his own bacterial imprint, which was stable during the entire study. Significant changes were observed in C. difficile positive individuals in the relative abundance of a few bacterial populations, including Lachnospiraceae and Verrucomicrobiaceae. A decrease of Akkermansia in positive subjects to the bacterium was repeatedly found. Conclusions A high C. difficile colonisation in nursing home residents was found, with a predominance of the hypervirulent PCR-ribotype 027. Positive C. difficile status is not associated with microbiota richness or biodiversity reduction in this study. The link between Akkermansia, gut inflammation and C. difficile colonisation merits further investigations. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1471-2180 |
| العلاقة: | urn:issn:1471-2180; https://orbi.uliege.be/handle/2268/202434Test; info:hdl:2268/202434; https://orbi.uliege.be/bitstream/2268/202434/1/art%253A10.1186%252Fs12866-016-0848-7.pdfTest; scopus-id:2-s2.0-84990838693; info:pmid:27716140 |
| DOI: | 10.1186/s12866-016-0848-7 |
| الإتاحة: | https://doi.org/10.1186/s12866-016-0848-7Test https://orbi.uliege.be/handle/2268/202434Test https://orbi.uliege.be/bitstream/2268/202434/1/art%253A10.1186%252Fs12866-016-0848-7.pdfTest |
| حقوق: | open access ; http://purl.org/coar/access_right/c_abf2Test ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.6C058001 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14712180 |
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| DOI: | 10.1186/s12866-016-0848-7 |