التفاصيل البيبلوغرافية
| العنوان: |
The Age-Dependent Role of Th22, Tc22, and Tc17 Cells in the Severity of Pneumonia in COVID-19 Immunopathogenesis. |
| المؤلفون: |
Cagan, Eren1,2 (AUTHOR), Tezcan, Gulcin3 (AUTHOR), Simsek, Abdurrahman1,4 (AUTHOR), Kizmaz, Muhammed Ali1,4 (AUTHOR), Dombaz, Fatma1,4 (AUTHOR), Asan, Ali5 (AUTHOR), Demir, H. Ibrahim1,4 (AUTHOR), Bal, Haldun1 (AUTHOR), Yoyen Ermis, Digdem1 (AUTHOR), Gorek Dilektasli, Aslı6 (AUTHOR), Kazak, Esra7 (AUTHOR), Akalin, E. Halis7 (AUTHOR), Oral, H. Barbaros1 (AUTHOR), Budak, Ferah1 (AUTHOR) fbudak@uludag.edu.tr |
| المصدر: |
Viral Immunology. May2022, Vol. 35 Issue 4, p318-327. 10p. |
| مصطلحات موضوعية: |
*MONONUCLEAR leukocytes, *CYTOTOXIC T cells, *T helper cells, *DENSITY gradient centrifugation, *COVID-19, *PNEUMONIA, *SYMPTOMS |
| مستخلص: |
Coronavirus disease 2019 (COVID-19) has clinical manifestations ranging from mild symptoms to respiratory failure, septic shock, and multi-organ failure. Lymphocytes are divided into different subtypes based on their cytokine production pattern. In this study, we investigated the role of cytokine expressions of CD4+ T (T helper [Th]1, Th2, Th17, Th22) and CD8+ T cell subtypes (T cytotoxic [Tc]1, Tc2, Tc17, Tc22) in the pathogenesis of COVID-19. Peripheral blood mononuclear cells (PBMCs) were extracted with Ficoll by density gradient centrifugation from blood samples of 180 COVID-19 patients (children and adults) and 30 healthy controls. PBMCs were stimulated with PMA and Ionomycin and treated with Brefeldin A in the fourth hour, and a 10-colored monoclonal antibody panel was evaluated at the end of the sixth hour using flow cytometry. According to our findings, the numbers of Th22 (CD3+, CD4+, and interleukin [IL]-22+) and Tc22 (CD3+, CD8+, IL-22+) cells increased in adult patients regardless of the level of pneumonia (mild, severe, or symptom-free) as compared with healthy controls (p < 0.05). In addition, the number of Tc17 (CD3+, CD8+, and IL-17A+) cells increased in low pneumonia and severe pneumonia groups compared with the healthy controls (p < 0.05). Both IL-22 and IL-17A production decreased during a follow-up within 6 weeks of discharge. Our findings suggest that the increase in only IL-22 expressed Tc22 cells in the 0–12 age group with a general symptom-free course and higher levels of Th22 and Tc22 in uncomplicated adult cases may indicate the protective effect of IL-22. On the contrary, the association between the severity of pneumonia and the elevation of Tc17 cells in adults may reveal the damaging effect of IL-22 when it is co-expressed with IL-17. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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