Longitudinal survey of Clostridium difficile presence and gut microbiota composition in a Belgian nursing home
| العنوان: | Longitudinal survey of Clostridium difficile presence and gut microbiota composition in a Belgian nursing home |
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| المؤلفون: | Johan Van Broeck, N. Korsak, Michel Delmée, Véronique Avesani, Philippe Brach, Cristina Rodríguez, Georges Daube, Bernard Taminiau |
| المساهمون: | UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale, UCL - (SLuc) Service de microbiologie |
| المصدر: | BMC Microbiology, Vol. 16, no. 1, p. 229 [1-12] (2016) BMC Microbiology |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Diarrhea, Male, 0301 basic medicine, Microbiology (medical), medicine.medical_specialty, 030106 microbiology, Multiple Loci VNTR Analysis, Gut flora, Microbiology, Feces, 03 medical and health sciences, Belgium, Risk Factors, Internal medicine, 16S rRNA gene analysis, Prevalence, medicine, Humans, Longitudinal Studies, Aged, Aged, 80 and over, biology, Clostridioides difficile, Lachnospiraceae, Akkermansia, Middle Aged, Clostridium difficile, biology.organism_classification, Anti-Bacterial Agents, Gastrointestinal Microbiome, Nursing Homes, Colonisation, Clostridium Infections, Female, C. difficile, Elderly care home residents, medicine.symptom, Research Article |
| الوصف: | Background Increasing age, several co-morbidities, environmental contamination, antibiotic exposure and other intestinal perturbations appear to be the greatest risk factors for C. difficile infection (CDI). Therefore, elderly care home residents are considered particularly vulnerable to the infection. The main objective of this study was to evaluate and follow the prevalence of C. difficile in 23 elderly care home residents weekly during a 4-month period. A C. difficile microbiological detection scheme was performed along with an overall microbial biodiversity study of the faeces content by 16S rRNA gene analysis. Results Seven out of 23 (30.4 %) residents were (at least one week) positive for C. difficile. C. difficile was detected in 14 out of 30 diarrhoeal samples (43.7 %). The most common PCR-ribotype identified was 027. MLVA showed that there was a clonal dissemination of C. difficile strains within the nursing home residents. 16S-profiling analyses revealed that each resident has his own bacterial imprint, which was stable during the entire study. Significant changes were observed in C. difficile positive individuals in the relative abundance of a few bacterial populations, including Lachnospiraceae and Verrucomicrobiaceae. A decrease of Akkermansia in positive subjects to the bacterium was repeatedly found. Conclusions A high C. difficile colonisation in nursing home residents was found, with a predominance of the hypervirulent PCR-ribotype 027. Positive C. difficile status is not associated with microbiota richness or biodiversity reduction in this study. The link between Akkermansia, gut inflammation and C. difficile colonisation merits further investigations. Electronic supplementary material The online version of this article (doi:10.1186/s12866-016-0848-7) contains supplementary material, which is available to authorized users. |
| تدمد: | 1471-2180 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73249b11b4cf3983154dce17330772cdTest https://doi.org/10.1186/s12866-016-0848-7Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....73249b11b4cf3983154dce17330772cd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712180 |
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