دورية أكاديمية
Cytoprotective Effects of Natural Highly Bio-Available Vegetable Derivatives on Human-Derived Retinal Cells
| العنوان: | Cytoprotective Effects of Natural Highly Bio-Available Vegetable Derivatives on Human-Derived Retinal Cells |
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| المؤلفون: | Munia, Ingrid, Gafray, Laurent, Bringer, Marie-Agnès, Goldschmidt, Pablo, Proukhnitzky, Lil, Jacquemot, Nathalie, Cercy, Christine, Ramchani Ben Otman, Khaoula, Errera, Marie Hélène, Ranchon-Cole, Isabelle |
| المساهمون: | Neuro-Dol (Neuro-Dol), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA), Centre des Sciences du Goût et de l'Alimentation Dijon (CSGA), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Bourgogne Franche-Comté COMUE (UBFC), Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts (CHNO), Sorbonne Université (SU), MDPI AG |
| المصدر: | ISSN: 2072-6643 ; Nutrients ; https://hal.inrae.fr/hal-02865916Test ; Nutrients, 2020, 12 (3), pp.879. ⟨10.3390/nu12030879⟩ ; https://www.mdpi.com/2072-6643/12/3/879Test. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2020 |
| المجموعة: | Université de Bourgogne (UB): HAL |
| مصطلحات موضوعية: | protection curcumin, resveratrol, lutein, antioxidant, apoptosis, autophagy, retina, damage, [SDV]Life Sciences [q-bio], [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| الوصف: | This article belongs to the Section Micronutrients and Human Health. ; International audience ; Retinal pigment epithelial cells are crucial for retina maintenance, making their cytoprotection an excellent way to prevent or slow down retinal degeneration. In addition, oxidative stress, inflammation, apoptosis, neovascularization, and/or autophagy are key pathways involved in degenerative mechanisms. Therefore, here we studied the effects of curcumin, lutein, and/or resveratrol on human retinal pigment epithelial cells (ARPE-19). Cells were incubated with individual or combined agent(s) before induction of (a) H2O2-induced oxidative stress, (b) staurosporin-induced apoptosis, (c) CoCl2-induced hypoxia, or (d) a LED-autophagy perturbator. Metabolic activity, cellular survival, caspase 3/7 activity (casp3/7), cell morphology, VEGF levels, and autophagy process were assessed. H2O2 provoked a reduction in cell survival, whereas curcumin reduced metabolic activity which was not associated with cell death. Cell death induced by H2O2 was significantly reduced after pre-treatment with curcumin and lutein, but not resveratrol. Staurosporin increased caspase-3/7 activity (689%) and decreased cell survival by 32%. Curcumin or lutein protected cells from death induced by staurosporin. Curcumin, lutein, and resveratrol were ineffective on the increase of caspase 3/7 induced by staurosporin. Pre-treatment with curcumin or lutein prevented LED-induced blockage of autophagy flux. Basal-VEGF release was significantly reduced by lutein. Therefore, lutein and curcumin showed beneficial protective effects on human-derived retinal cells against several insults. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/32214021; hal-02865916; https://hal.inrae.fr/hal-02865916Test; https://hal.inrae.fr/hal-02865916/documentTest; https://hal.inrae.fr/hal-02865916/file/nutrients-12-00879.pdfTest; PUBMED: 32214021; PUBMEDCENTRAL: PMC7146218; WOS: 000531831000288 |
| DOI: | 10.3390/nu12030879 |
| الإتاحة: | https://doi.org/10.3390/nu12030879Test https://hal.inrae.fr/hal-02865916Test https://hal.inrae.fr/hal-02865916/documentTest https://hal.inrae.fr/hal-02865916/file/nutrients-12-00879.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.657CE2D1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/nu12030879 |
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