Characterisation and preliminary functional analysis of N-acetyltransferase 13 from Schistosoma japonicum
| العنوان: | Characterisation and preliminary functional analysis of N-acetyltransferase 13 from Schistosoma japonicum |
|---|---|
| المؤلفون: | Jinming Liu, Xingang Yu, Hao Li, Yang Hong, Ke Lu, Jiaojiao Lin, Cheng Chen, Zhiqiang Fu, Tang Yalan, Kerou Zhou, Guo Qingqing, Qinghong Guo, Jia Jing |
| المصدر: | BMC Veterinary Research, Vol 17, Iss 1, Pp 1-11 (2021) BMC Veterinary Research |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, Small interfering RNA, DNA, Complementary, Veterinary medicine, Development, Gene Expression Regulation, Enzymologic, Schistosoma japonicum, Mice, Random Allocation, Acetyltransferases, RNA interference, Complementary DNA, parasitic diseases, SF600-1100, Animals, Gene Silencing, RNA, Messenger, Cloning, Molecular, chemistry.chemical_classification, Mice, Inbred BALB C, Vaccines, SjNAT13, General Veterinary, biology, Research, Reproduction, Helminth Proteins, General Medicine, biology.organism_classification, Molecular biology, In vitro, Amino acid, Open reading frame, chemistry, Schistosome, N-acetyltransferase, Schistosomiasis japonica, RNAi, biology.protein, Female, Antibody |
| الوصف: | Background N-acetyltransferase 13 (NAT13) is a probable catalytic component of the ARD1A-NARG1 complex possessing alpha (N-terminal) acetyltransferase activity. Results In this study, a full-length complementary DNA (cDNA) encoding Schistosoma japonicum NAT13 (SjNAT13) was isolated from schistosome cDNAs. The 621 bp open reading frame of SjNAT13 encodes a polypeptide of 206 amino acids. Real-time PCR analysis revealed SjNAT13 expression in all tested developmental stages. Transcript levels were highest in cercariae and 21-day-old worms, and higher in male adult worms than female adult worms. The rSjNAT13 protein induced high levels of anti-rSjNAT13 IgG antibodies. In two independent immunoprotection trials, rSjNAT13 induced 24.23% and 24.47% reductions in the numbers of eggs in liver. RNA interference (RNAi) results showed that small interfering RNA (siRNA) Sj-514 significantly reduced SjNAT13 transcript levels in worms and decreased egg production in vitro. Conclusions Thus, rSjNAT13 might play an important role in the development and reproduction of schistosomes. |
| اللغة: | English |
| تدمد: | 1746-6148 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ba966e9df4cb9ae355069fc741bcb3fTest https://doaj.org/article/8e8ab90f67fc40c48d282eff9111c3a4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4ba966e9df4cb9ae355069fc741bcb3f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17466148 |
|---|