دورية أكاديمية
A comparative study of the effects of 3 testicular toxicants in cultures of seminiferous tubules of rats or of domestic cats (veterinary waste): An alternative method for reprotoxicology
| العنوان: | A comparative study of the effects of 3 testicular toxicants in cultures of seminiferous tubules of rats or of domestic cats (veterinary waste): An alternative method for reprotoxicology |
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| المؤلفون: | Blondet, Antonine, Martin, Guillaume, Durand, Philippe, Perrard, Marie-Hélène |
| المساهمون: | Kallistem SAS, Partenaires INRAE, VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Institut cellule souche et cerveau / Stem Cell and Brain Research Institute (SBRI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| المصدر: | ISSN: 0887-2333. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2022 |
| المجموعة: | HAL Lyon 1 (University Claude Bernard Lyon 1) |
| مصطلحات موضوعية: | Spermatogenesis, In vitro model, Replacement of animals, Veterinary waste, Prepubertal domestic cat, Reproductive toxicology, [SDV.TOX]Life Sciences [q-bio]/Toxicology |
| الوصف: | International audience ; In response to the EU cosmetics directive regulation and REACH legislation which encourage cell culture methods in order to reduce or replace the use of animals in toxicology studies, we settled the culture of prepubertal domestic cat seminiferous tubules in our validated BioAlter® model, usually used with prepubertal rat, called here BioAlter®-rat, by opposition to BioAlter®-cat settled here. We carried out a comparative study on the effects of 3 testicular toxicants, 1,3-dinitrobenzene at 60 μM, 2-methoxyacetic acid at 2.5 mM and carbendazim at 50 nM or 500 nM in both BioAlter®-cat and BioAlter®-rat over a 3-week culture period. Sertoli cell or each germ cell populations as well as the levels of Sertoli cell or germ cell specific mRNAs were studied. The harmful effects of the 3 toxicants on pre-meiotic, meiotic and post-meiotic cell numbers and on Sertoli or germ cell specific mRNAs were clearly observed in the two species, even if there might be some small differences in the intensity of the effects on some of the studied parameters. Hence, BioAlter®-cat might be a solution to the requirements of the EU cosmetics directive and REACH legislation for male reproductive toxicology studies. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-03761565; https://hal.science/hal-03761565Test; https://hal.science/hal-03761565/documentTest; https://hal.science/hal-03761565/file/Chat%20TIV%202022.pdfTest |
| DOI: | 10.1016/j.tiv.2022.105397 |
| الإتاحة: | https://doi.org/10.1016/j.tiv.2022.105397Test https://hal.science/hal-03761565Test https://hal.science/hal-03761565/documentTest https://hal.science/hal-03761565/file/Chat%20TIV%202022.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.34E599B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.tiv.2022.105397 |
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