دورية أكاديمية
Second Harmonic Generation microscopy reveals collagen fibres are more organised in the cervix of postmenopausal women
| العنوان: | Second Harmonic Generation microscopy reveals collagen fibres are more organised in the cervix of postmenopausal women |
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| المؤلفون: | Brenda F. Narice, Nicola H. Green, Sheila MacNeil, Dilly Anumba |
| المصدر: | Reproductive Biology and Endocrinology, Vol 14, Iss 1, Pp 1-8 (2016) |
| بيانات النشر: | BMC, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Gynecology and obstetrics LCC:Reproduction |
| مصطلحات موضوعية: | Second harmonic generation microscopy, Collagen, Cervix, Menopause, Human, Alignment, Gynecology and obstetrics, RG1-991, Reproduction, QH471-489 |
| الوصف: | Abstract Background During labour, the cervix undergoes a series of changes to allow the passage of the fetoplacental unit. While this visible transformation is well-described, the underlying and causative microscopic changes, in which collagen plays a major role, are poorly understood and difficult to visualise. Recent studies in mice and humans have shown that Second Harmonic Generation (SHG) microscopy, a non-destructive imaging technique, can detect changes in the cervical collagen. However, the question of whether SHG can identify changes in the arrangement of cervical collagen at different physiological stages still needs addressing. Therefore, this study aimed to compare the cervical collagen alignment between pre- and postmenopausal women using SHG and to generate proof-of-concept data prior to assessing this technique in pregnancy. Methods Cervical biopsies from premenopausal (n = 4) and postmenopausal (n = 4) multiparous women undergoing hysterectomy for benign conditions were cross-sectionally scanned using an upright confocal microscope. SHG images were collected in Z-stacks and qualitatively evaluated using semi-quantitative scoring (0–3 in ascending degree of alignment) by assessors who were unaware of the classification of the SHG images, and quantitatively, using 2D Fourier transformation analysis. The dominant orientation and difference in dispersion of collagen fibres in each z-stack (X ± SD) was calculated and compared between groups. Results Qualitatively, collagen fibres appeared more organised in postmenopausal women, [premenopausal: median 0, range (0–1), postmenopausal: median 1.25, range (1–3); X 2 (df = 5) = 19.35, p = 0.002]. Quantitatively, there was a statistically significant difference in collagen fibre dispersion between premenopausal (5.39° ± 12.68°) and postmenopausal women (−1.58° ± 8.24°), [Welch’s t-test (245.54) = 5.54, p |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1477-7827 |
| العلاقة: | http://link.springer.com/article/10.1186/s12958-016-0204-7Test; https://doaj.org/toc/1477-7827Test |
| DOI: | 10.1186/s12958-016-0204-7 |
| الوصول الحر: | https://doaj.org/article/fda5d6add1b9453f8a8d42254593536bTest |
| رقم الانضمام: | edsdoj.fda5d6add1b9453f8a8d42254593536b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14777827 |
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| DOI: | 10.1186/s12958-016-0204-7 |