دورية أكاديمية
MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity
| العنوان: | MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity |
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| المؤلفون: | Peirsman, A., Blondeel, E., Ahmed, T., Anckaert, J., Audenaert, D., Boterberg, T., Buzas, K., Carragher, N., Castellani, G., Castro, F., Dangles-Marie, V., Dawson, J., De Tullio, Pascal, De Vlieghere, E., Dedeyne, S., Depypere, H., Diosdi, A., Dmitriev, R. I., Dolznig, H., Fischer, S., Gespach, C., Goossens, V., Heino, J., Hendrix, A., Horvath, P., Kunz-Schughart, L. A., Maes, S., Mangodt, C., Mestdagh, P., Michlíková, S., Oliveira, M. J., Pampaloni, F., Piccinini, F., Pinheiro, C., Rahn, J., Robbins, S. M., Siljamäki, E., Steigemann, P., Sys, G., Takayama, S., Tesei, A., Tulkens, J., Van Waeyenberge, M., Vandesompele, J., Wagemans, G., Weindorfer, C., Yigit, N., Zablowsky, N., Zanoni, M., Blondeel, P., De Wever, O. |
| المصدر: | Nature Methods, 18 (11), 1294-1303 (2021) |
| بيانات النشر: | Nature Research |
| سنة النشر: | 2021 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | Biomarkers, Tumor, Cell Culture Techniques, Cell Proliferation, Gene Expression Regulation, Neoplastic, Humans, Knowledge Bases, Neoplasms, Reproducibility of Results, RNA-Seq, Software, Spheroids, Cellular, Tumor Cells, Cultured, Tumor Microenvironment, Human health sciences, Sciences de la santé humaine |
| الوصف: | peer reviewed ; Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation of this knowledgebase identified heterogeneity in the methodological setup of spheroids. Empirical evaluation and interlaboratory validation of selected variations in spheroid methodology revealed diverse impacts on spheroid metrics. To facilitate interpretation, stimulate transparency and increase awareness, the Consortium defines the MISpheroID string, a minimum set of experimental parameters required to report spheroid research. Thus, MISpheroID combines a valuable resource and a tool for three-dimensional cellular models to mine experimental parameters and to improve reproducibility. © 2021, The Author(s). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1548-7091 1548-7105 |
| العلاقة: | urn:issn:1548-7091; urn:issn:1548-7105; https://orbi.uliege.be/handle/2268/267666Test; info:hdl:2268/267666; scopus-id:2-s2.0-85118431713; info:pmid:34725485 |
| DOI: | 10.1038/s41592-021-01291-4 |
| الإتاحة: | https://doi.org/10.1038/s41592-021-01291-4Test https://orbi.uliege.be/handle/2268/267666Test |
| حقوق: | restricted access ; http://purl.org/coar/access_right/c_16ecTest ; info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.4466C494 |
| قاعدة البيانات: | BASE |
| تدمد: | 15487091 15487105 |
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| DOI: | 10.1038/s41592-021-01291-4 |