دورية أكاديمية
Differential expression of NMDA and AMPA/KA receptor subunits in the inferior olive of postnatal rats
| العنوان: | Differential expression of NMDA and AMPA/KA receptor subunits in the inferior olive of postnatal rats |
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| المؤلفون: | Chan, YS, Guan, ZL, Li, C, Tse, YC, Chen, LW, Shum, DKY, Yung, KKL, Lai, CH |
| بيانات النشر: | //www.elsevier.com/locate/brainres Netherlands |
| سنة النشر: | 2006 |
| المجموعة: | University of Hong Kong: HKU Scholars Hub |
| مصطلحات موضوعية: | AMPA/KA receptor, Co-localization, Immunohistochemistry, Inferior olive, NMDA receptor, Protein Subunits - metabolism, Receptors, N-Methyl-D-Aspartate - metabolism, Kainic Acid - metabolism, AMPA - metabolism, Rats, Olivary Nucleus - growth & development - physiology, Animals, Aging |
| الوصف: | We have employed immunohistochemistry to determine the expression patterns of receptor subunits of N-methyl-d-aspartate (NMDA-NR1 and NR2A/B) and α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid/kainic acid (AMPA/KA-GluR1, GluR2, GluR2/3, GluR4, and GluR5/6/7) in the inferior olive of postnatal rats up to adulthood. Immunoreactivity for distinct receptor subunits was predominantly localized in the soma and dendrites of neurons. Semi-quantification showed that the overall immunoreactivity in the inferior olive of adults was intense for GluR1, moderate for NR1 and NR2A/B, and low for GluR2, GluR2/3, GluR4, and GluR5/6/7. At P7, GluR1 was restricted to the dorsomedial cell column, subnucleus beta, principal nucleus and ventrolateral protrusion while the other subunits were found in all subnuclei of the inferior olive. The immunoreactivities for all glutamate receptor subunits ranged from low to moderate. As the rats matured, the immunoreactivity of GluR4 decreased after the second postnatal week, while those of the other subunits showed a general trend of increase, reaching adult level during the third postnatal week. Double immunofluorescence revealed that all NR1-containing neurons exhibited NR2A/B immunoreactivity, indicating that native NMDA receptors comprise of hetero-oligomeric combinations of NR1 and NR2A/B. Furthermore, co-localization of NMDA and AMPA/KA receptor subunits was demonstrated in individual neurons of the inferior olive. All NR1-containing neurons exhibited GluR1 immunoreactivity, and all NR2A/B-containing neurons showed GluR5/6/7 immunoreactivity. Our data suggest that NMDA and AMPA/KA receptors are involved in glutamate-mediated neurotransmission, contributing to synaptic plasticity and reorganization of circuitry in the inferior olive during postnatal development. © 2005 Elsevier B.V. All rights reserved. ; link_to_subscribed_fulltext |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0006-8993 |
| العلاقة: | Brain Research; http://www.scopus.com/mlt/select.url?eid=2-s2.0-31144455841&selection=ref&src=s&origin=recordpageTest; Brain Research, 2006, v. 1067 n. 1, p. 103-114; 114; 121193; WOS:000235356500013; http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-8993&volume=1067&issue=1&spage=103&epage=114&date=2006&atitle=Differential+expression+of+NMDA+and+AMPA/KA+receptor+subunits+in+the+inferior+olive+of+postnatal+ratsTest; eid_2-s2.0-31144455841; 103; http://hdl.handle.net/10722/68332Test; 1067 |
| DOI: | 10.1016/j.brainres.2005.10.054 |
| الإتاحة: | https://doi.org/10.1016/j.brainres.2005.10.054Test http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-8993&volume=1067&issue=1&spage=103&epage=114&date=2006&atitle=Differential+expression+of+NMDA+and+AMPA/KA+receptor+subunits+in+the+inferior+olive+of+postnatal+ratsTest http://hdl.handle.net/10722/68332Test |
| حقوق: | Brain Research. Copyright © Elsevier BV. |
| رقم الانضمام: | edsbas.DED9B372 |
| قاعدة البيانات: | BASE |
| تدمد: | 00068993 |
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| DOI: | 10.1016/j.brainres.2005.10.054 |