دورية أكاديمية
Plasmodium Cysteine Repeat Modular Proteins 3 and 4 are essential for malaria parasite transmission from the mosquito to the host
| العنوان: | Plasmodium Cysteine Repeat Modular Proteins 3 and 4 are essential for malaria parasite transmission from the mosquito to the host |
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| المؤلفون: | Mota Maria M, Ramesar Jai, Moore Sally G, Augustijn Kevin D, Douradinha Bruno, Waters Andrew P, Janse Chris J, Thompson Joanne |
| المصدر: | Malaria Journal, Vol 10, Iss 1, p 71 (2011) |
| بيانات النشر: | BMC |
| سنة النشر: | 2011 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Arctic medicine. Tropical medicine, RC955-962, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Background The Plasmodium Cysteine Repeat Modular Proteins (PCRMP) are a family of four conserved proteins of malaria parasites, that contain a number of motifs implicated in host-parasite interactions. Analysis of mutants of the rodent parasite Plasmodium berghei lacking expression of PCRMP1 or 2 showed that these proteins are essential for targeting of P. berghei sporozoites to the mosquito salivary gland and, hence, for transmission from the mosquito to the mouse. Methods In this work, the role of the remaining PCRMP family members, PCRMP3 and 4, has been investigated throughout the Plasmodium life cycle by generation and analysis of P. berghei gene deletion mutants, Δ pcrmp 3 and Δ pcrmp 4. The role of PCRMP members during the transmission and hepatic stages of the Plasmodium lifecycle has been evaluated by light- and electron microscopy and by analysis of liver stage development in HEPG2 cells in vitro and by infecting mice with mutant sporozoites. In addition, mice were immunized with live Δ pcrmp 3 and Δ pcrmp 4 sporozoites to evaluate their immunization potential as a genetically-attenuated parasite-based vaccine. Results Disruption of pcrmp3 and pcrmp4 in P. berghei revealed that they are also essential for transmission of the parasite through the mosquito vector, although acting in a distinct way to pbcrmp1 and 2 . Mutants lacking expression of PCRMP3 or PCRMP4 show normal blood stage development and oocyst formation in the mosquito and develop into morphologically normal sporozoites, but these have a defect in egress from oocysts and do not enter the salivary glands. Sporozoites extracted from oocysts perform gliding motility and invade and infect hepatocytes but do not undergo further development and proliferation. Furthermore, the study shows that immunization with Δ crmp3 and Δ crmp4 sporozoites does not confer protective immunity upon subsequent challenge. Conclusions PCRMP3 and 4 play multiple roles during the Plasmodium life cycle; they are essential for the establishment of sporozoite ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1475-2875 |
| العلاقة: | http://www.malariajournal.com/content/10/1/71Test; https://doaj.org/toc/1475-2875Test; https://doaj.org/article/18a4863b66944a7a8929e887655d829dTest |
| DOI: | 10.1186/1475-2875-10-71 |
| الإتاحة: | https://doi.org/10.1186/1475-2875-10-71Test https://doaj.org/article/18a4863b66944a7a8929e887655d829dTest |
| رقم الانضمام: | edsbas.E17F34B3 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14752875 |
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| DOI: | 10.1186/1475-2875-10-71 |