دورية أكاديمية
The RNA-binding protein Mex3b regulates the spatial organization of the Rap1 pathway
| العنوان: | The RNA-binding protein Mex3b regulates the spatial organization of the Rap1 pathway |
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| المؤلفون: | Le Borgne, Maïlys, Chartier, Nicolas, Buchet-Poyau, Karine, Destaing, Olivier, Faurobert, Eva, Thibert, Chantal, Rouault, Jean-Pierre, Courchet, Julien, Nègre, Didier, Bouvard, Daniel, Albigès-Rizo, Corinne, Rousseaux, Sophie, Khochbin, Saadi, Segretain, Dominique, Crépieux, Pascale, Guillou, Florian, Durand, Philippe, Perrard, Marie-Heĺeǹe, Billaud, Marc |
| المساهمون: | Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-Centre Hospitalier Universitaire CHU Grenoble (CHUGA)-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Hospices Civils de Lyon (HCL), Dynamique des systèmes d'adhérence et différenciation (DySAD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Apoptose Cancer et Développement, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Department of Neuroscience New York, Columbia University New York, Plateau technique de Vectorologie, UMS3444/US8, Unité de Virologie Humaine, Institut National de la Santé et de la Recherche Médicale (INSERM), Virologie humaine, École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'études de la différenciation et de l'adhérence cellulaires (LEDAC), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Adaptation et pathogénie des micro-organismes Grenoble (LAPM), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Physiologie de la reproduction et des comportements Nouzilly (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation Saumur (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Communications cellulaires et différenciation, Université de Lyon-Université de Lyon-Hospices Civils de Lyon (HCL)-Hôpital Debrousse, Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Neurodegenerescence et Plasticite, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | ISSN: 0950-1991. |
| بيانات النشر: | HAL CCSD Company of Biologists |
| سنة النشر: | 2014 |
| المجموعة: | Université de Versailles Saint-Quentin-en-Yvelines: HAL-UVSQ |
| مصطلحات موضوعية: | RNA-binding proteins, Rap1, Phagocytosis, Sertoli cell, Mouse, MEX-3, [SDV]Life Sciences [q-bio], [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BDD.MOR]Life Sciences [q-bio]/Development Biology/Morphogenesis |
| الوصف: | International audience ; The four related mammalian MEX-3 RNA-binding proteins are evolutionarily conserved molecules for which the in vivo functions have not yet been fully characterized. Here, we report that male mice deficient for the gene encoding Mex3b are subfertile. Seminiferous tubules of Mex3b-deficient mice are obstructed as a consequence of the disrupted phagocytic capacity of somatic Sertoli cells. In addition, both the formation and the integrity of the blood-testis barrier are compromised owing to mislocalization of N-cadherin and connexin 43 at the surface of Sertoli cells. We further establish that Mex3b acts to regulate the cortical level of activated Rap1, a small G protein controlling phagocytosis and cell-cell interaction, through the activation and transport of Rap1GAP. The active form of Rap1 (Rap1-GTP) is abnormally increased at the membrane cortex and chemically restoring Rap1-GTP to physiological levels rescues the phagocytic and adhesion abilities of Sertoli cells. Overall, these findings implicate Mex3b in the spatial organization of the Rap1 pathway that orchestrates Sertoli cell functions. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-02336153; https://hal.science/hal-02336153Test; https://hal.science/hal-02336153/documentTest; https://hal.science/hal-02336153/file/LEBORGNE%20ET%20AL%20DEVPT%202014.pdfTest; WOS: 000336126400012 |
| DOI: | 10.1242/dev.108514 |
| الإتاحة: | https://doi.org/10.1242/dev.108514Test https://hal.science/hal-02336153Test https://hal.science/hal-02336153/documentTest https://hal.science/hal-02336153/file/LEBORGNE%20ET%20AL%20DEVPT%202014.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.2104809A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1242/dev.108514 |
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