دورية أكاديمية
Integrated Analysis of Contractile Kinetics, Force Generation, and Electrical Activity in Single Human Stem Cell-Derived Cardiomyocytes
| العنوان: | Integrated Analysis of Contractile Kinetics, Force Generation, and Electrical Activity in Single Human Stem Cell-Derived Cardiomyocytes |
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| المؤلفون: | Jan David Kijlstra, Dongjian Hu, Nikhil Mittal, Eduardo Kausel, Peter van der Meer, Arman Garakani, Ibrahim J. Domian |
| المصدر: | Stem Cell Reports, Vol 5, Iss 6, Pp 1226-1238 (2015) |
| بيانات النشر: | Elsevier, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | The quantitative analysis of cardiomyocyte function is essential for stem cell-based approaches for the in vitro study of human cardiac physiology and pathophysiology. We present a method to comprehensively assess the function of single human pluripotent stem cell-derived cardiomyocyte (hPSC-CMs) through simultaneous quantitative analysis of contraction kinetics, force generation, and electrical activity. We demonstrate that statistical analysis of movies of contracting hPSC-CMs can be used to quantify changes in cellular morphology over time and compute contractile kinetics. Using a biomechanical model that incorporates substrate stiffness, we calculate cardiomyocyte force generation at single-cell resolution and validate this approach with conventional traction force microscopy. The addition of fluorescent calcium indicators or membrane potential dyes allows the simultaneous analysis of contractility and calcium handling or action potential morphology. Accordingly, our approach has the potential for broad application in the study of cardiac disease, drug discovery, and cardiotoxicity screening. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2213-6711 |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S2213671115003148Test; https://doaj.org/toc/2213-6711Test |
| DOI: | 10.1016/j.stemcr.2015.10.017 |
| الوصول الحر: | https://doaj.org/article/bce05f17356e4bae8247c69dfc6c9ee9Test |
| رقم الانضمام: | edsdoj.bce05f17356e4bae8247c69dfc6c9ee9 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22136711 |
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| DOI: | 10.1016/j.stemcr.2015.10.017 |