التفاصيل البيبلوغرافية
| العنوان: |
Frequenin/NCS-1 and the Ca2+-channel α1-subunit co-regulate synaptic transmission and nerve-terminal growth. |
| المؤلفون: |
Dason, Jeffrey S., Romero-Pozuelo, Jesús, Marin, Leo, Iyengar, Balaji G., Klose, Markus K., Ferrús, Alberto, Atwood, Harold L. |
| المصدر: |
Journal of Cell Science; 11/15/2009, Vol. 122 Issue 22, p11-11, 1p |
| مصطلحات موضوعية: |
DROSOPHILA, REGULATION of neural transmission, GENOTYPE-environment interaction, PHENOTYPES, CARRIER proteins, TERMINAL nerve, NEURAL circuitry |
| مستخلص: |
Drosophila Frequenin (Frq) and its mammalian and worm homologue, NCS-1, are Ca2+-binding proteins involved in neurotransmission. Using site-specific recombination in Drosophila, we created two deletions that removed the entire frq1 gene and part of the frq2 gene, resulting in no detectable Frq protein. Frq-null mutants were viable, but had defects in larval locomotion, deficient synaptic transmission, impaired Ca2+ entry and enhanced nerve-terminal growth. The impaired Ca2+ entry was sufficient to account for reduced neurotransmitter release. We hypothesized that Frq either modulates Ca2+ channels, or that it regulates the PI4Kβ pathway as described in other organisms. To determine whether Frq interacts with PI4Kβ with consequent effects on Ca2+ channels, we first characterized a PI4Kβ-null mutant and found that PI4Kβ was dispensable for synaptic transmission and nerve-terminal growth. Frq gain-of-function phenotypes remained present in a PI4Kβ-null background. We conclude that the effects of Frq are not due to an interaction with PI4Kβ. Using flies that were trans-heterozygous for a null frq allele and a null cacophony (encoding the α1-subunit of voltage-gated Ca2+ channels) allele, we show a synergistic effect between these proteins in neurotransmitter release. Gain-of-function Frq phenotypes were rescued by a hypomorphic cacophony mutation. Overall, Frq modulates Ca2+ entry through a functional interaction with the α1 voltage-gated Ca2+-channel subunit; this interaction regulates neurotransmission and nerve-terminal growth. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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