دورية أكاديمية
A cis-acting diversification activator both necessary and sufficient for AID-mediated hypermutation.
العنوان: | A cis-acting diversification activator both necessary and sufficient for AID-mediated hypermutation. |
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المؤلفون: | Artem Blagodatski, Vera Batrak, Sabine Schmidl, Ulrike Schoetz, Randolph B Caldwell, Hiroshi Arakawa, Jean-Marie Buerstedde |
المصدر: | PLoS Genetics, Vol 5, Iss 1, p e1000332 (2009) |
بيانات النشر: | Public Library of Science (PLoS), 2009. |
سنة النشر: | 2009 |
المجموعة: | LCC:Genetics |
مصطلحات موضوعية: | Genetics, QH426-470 |
الوصف: | Hypermutation of the immunoglobulin (Ig) genes requires Activation Induced cytidine Deaminase (AID) and transcription, but it remains unclear why other transcribed genes of B cells do not mutate. We describe a reporter transgene crippled by hypermutation when inserted into or near the Ig light chain (IgL) locus of the DT40 B cell line yet stably expressed when inserted into other chromosomal positions. Step-wise deletions of the IgL locus revealed that a sequence extending for 9.8 kilobases downstream of the IgL transcription start site confers the hypermutation activity. This sequence, named DIVAC for diversification activator, efficiently activates hypermutation when inserted at non-Ig loci. The results significantly extend previously reported findings on AID-mediated gene diversification. They show by both deletion and insertion analyses that cis-acting sequences predispose neighboring transcription units to hypermutation. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1553-7390 1553-7404 |
العلاقة: | http://europepmc.org/articles/PMC2607555?pdf=renderTest; https://doaj.org/toc/1553-7390Test; https://doaj.org/toc/1553-7404Test |
DOI: | 10.1371/journal.pgen.1000332 |
الوصول الحر: | https://doaj.org/article/079156d0041a4c42acafaec995b75adaTest |
رقم الانضمام: | edsdoj.079156d0041a4c42acafaec995b75ada |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 15537390 15537404 |
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DOI: | 10.1371/journal.pgen.1000332 |