التفاصيل البيبلوغرافية
| العنوان: |
Dynamic association of the H3K64 trimethylation mark with genes encoding exported proteins in Plasmodium falciparum. |
| المؤلفون: |
Jabeena, C. A.1,2, Govindaraju, Gayathri1,2, Rawat, Mukul3, Gopi, Soundhararajan4, Sethumadhavan, Devadathan Valiyamangalath1,2, Jaleel, Abdul5, Sasankan, Dhakshmi1, Karmodiya, Krishanpal3, Rajavelu, Arumugam1 arajavelu@rgcb.res.in |
| المصدر: |
Journal of Biological Chemistry. Jan-Jun2021, Vol. 296, p1-18. 18p. |
| مصطلحات موضوعية: |
*PLASMODIUM falciparum, *REGULATOR genes, *GENE expression, *PROTEINS, *GENE families, *IMMUNOPRECIPITATION, *MALARIA |
| مستخلص: |
Epigenetic modifications have emerged as critical regulators of virulence genes and stage-specific gene expression in Plasmodium falciparum. However, the specific roles of histone core epigenetic modifications in regulating the stage-specific gene expression are not well understood. In this study, we report an unconventional trimethylation at lysine 64 on histone 3 (H3K64me3) and characterize its functional relevance in P. falciparum. We show that PfSET4 and PfSET5 proteins of P. falciparum methylate H3K64 and that they prefer the nucleosome as a substrate over free histone 3 proteins. Structural analysis of PfSET5 revealed that it interacts with the nucleosome as a dimer. The H3K64me3 mark is dynamic, being enriched in the ring and trophozoite stages and drastically reduced in the schizont stages. Stage-specific global chromatin immunoprecipitation -sequencing analysis of the H3K64me3 mark revealed the selective enrichment of this methyl mark on the genes of exported family proteins in the ring and trophozoite stages and a significant reduction of the same in the schizont stages. Collectively, our data identify a novel epigenetic mark that is associated with the subset of genes encoding for exported proteins, which may regulate their expression in different stages of P. falciparum. [ABSTRACT FROM AUTHOR] |
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