دورية أكاديمية
Building the vertebrate codex using the gene breaking protein trap library /
| العنوان: | Building the vertebrate codex using the gene breaking protein trap library / |
|---|---|
| المؤلفون: | Ichino, Noriko, Serres, Makayla R., Urban, Rhianna M., Urban, Mark D., Treichel, Anthony J., Schaefbauer, Kyle J., Greif, Lauren E., Varshney, Gaurav K., Skuster, Kimberly J., McNulty, Melissa S., Daby, Camden L., Wang, Ying, Liao, Hsin-Kai, El-Rass, Suzan, Ding, Yonghe, Liu, Weibin, Anderson, Jennifer L., Wishman, Mark D., Sabharwal, Ankit, Schimmenti, Lisa A., Sivasubbu, Sridhar, Balčiūnas, Darius, Hammerschmidt, Matthias, Farber, Steven A., Wen, Xiao-Yan, Xu, Xiaolei, McGrail, Maura, Essner, Jeffrey J., Burgess, Shawn M., Clark, Karl J., Ekker, Stephen C. |
| المصدر: | eLife., Cambridge : eLife Sciences Publications Ltd, 2020, vol. 9, art. no. e54572, p. [1-31]. ; eISSN 2050-084X |
| سنة النشر: | 2020 |
| المجموعة: | Vilnius University Virtual Library (VU VL) / Vilniaus universitetas virtuali biblioteka |
| مصطلحات موضوعية: | Disease model, Gene reversion, Gene-break transposon, Human genetic disorders, Light sheet microscopy, Protein trap, Zebrafish |
| الوصف: | One key bottleneck in understanding the human genome is the relative under-characterization of 90% of protein coding regions. We report a collection of 1,200 transgenic zebrafish strains made with the gene-break transposon (GBT) protein trap to simultaneously report and reversibly knockdown the tagged genes. Protein trap-associated mRFP expression shows previously undocumented expression of 35% and 90% of cloned genes at 2 and 4 days post-fertilization, respectively. Further, investigated alleles regularly show 99% gene-specific mRNA knockdown. Homozygous GBT animals in ryr1b, fras1, tnnt2a, edar and hmcn1 phenocopied established mutants. 204 cloned lines trapped diverse proteins, including 64 orthologs of human disease-associated genes with 40 as potential new disease models. Severely reduced skeletal muscle Ca2+ transients in GBT ryr1b homozygous animals validated the ability to explore molecular mechanisms of genetic diseases. This GBT system facilitates novel functional genome annotation towards understanding cellular and molecular underpinnings of vertebrate biology and human disease. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://epublications.vu.lt/object/elaba:141275362/141275362.pdfTest; https://repository.vu.lt/VU:ELABAPDB141275362&prefLang=en_USTest |
| الإتاحة: | https://doi.org/10.7554/ELIFE.54572Test https://repository.vu.lt/VU:ELABAPDB141275362&prefLang=en_USTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.3684CEC3 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |