التفاصيل البيبلوغرافية
| العنوان: |
Single-Domain Antibodies for Targeting, Detection, and In Vivo Imaging of Human CD4+ Cells. |
| المؤلفون: |
Traenkle, Bjoern, Kaiser, Philipp D., Pezzana, Stefania, Richardson, Jennifer, Gramlich, Marius, Wagner, Teresa R., Seyfried, Dominik, Weldle, Melissa, Holz, Stefanie, Parfyonova, Yana, Nueske, Stefan, Scholz, Armin M., Zeck, Anne, Jakobi, Meike, Schneiderhan-Marra, Nicole, Schaller, Martin, Maurer, Andreas, Gouttefangeas, Cécile, Kneilling, Manfred, Pichler, Bernd J. |
| المصدر: |
Frontiers in Immunology; 12/9/2021, Vol. 12, p1-17, 17p |
| مصطلحات موضوعية: |
POSITRON emission tomography, MAGNETIC resonance imaging, IMMUNOGLOBULINS, LABORATORY mice, CD4 antigen, MOLECULAR probes |
| مستخلص: |
The advancement of new immunotherapies necessitates appropriate probes to monitor the presence and distribution of distinct immune cell populations. Considering the key role of CD4+ cells in regulating immunological processes, we generated novel single-domain antibodies [nanobodies (Nbs)] that specifically recognize human CD4. After in-depth analysis of their binding properties, recognized epitopes, and effects on T-cell proliferation, activation, and cytokine release, we selected CD4-specific Nbs that did not interfere with crucial T-cell processes in vitro and converted them into immune tracers for noninvasive molecular imaging. By optical imaging, we demonstrated the ability of a high-affinity CD4-Nb to specifically visualize CD4+ cells in vivo using a xenograft model. Furthermore, quantitative high-resolution immune positron emission tomography (immunoPET)/MR of a human CD4 knock-in mouse model showed rapid accumulation of 64Cu-radiolabeled CD4-Nb1 in CD4+ T cell-rich tissues. We propose that the CD4-Nbs presented here could serve as versatile probes for stratifying patients and monitoring individual immune responses during personalized immunotherapy in both cancer and inflammatory diseases. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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