Antimicrobial susceptibility against metronidazole and carbapenem in clinical anaerobic isolates from Pakistan
| العنوان: | Antimicrobial susceptibility against metronidazole and carbapenem in clinical anaerobic isolates from Pakistan |
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| المؤلفون: | Afia Zafar, Yusra Shafquat, Joveria Farooqi, Kiran Mehmood, Kauser Jabeen, Rumina Hasan, Seema Irfan |
| المصدر: | Antimicrobial Resistance and Infection Control Antimicrobial Resistance and Infection Control, Vol 8, Iss 1, Pp 1-7 (2019) |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Microbiology (medical), medicine.medical_specialty, Imipenem, Carbapenem, Risk factors associated with resistance, 030106 microbiology, Antimicrobial susceptibility, Microbial Sensitivity Tests, Drug resistance, lcsh:Infectious and parasitic diseases, Bacteria, Anaerobic, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Medical microbiology, Risk Factors, Metronidazole, Internal medicine, Drug Resistance, Bacterial, Humans, Medicine, lcsh:RC109-216, Pakistan, Pharmacology (medical), 030212 general & internal medicine, Anaerobic resistance to carbapenem, business.industry, Research, Public Health, Environmental and Occupational Health, Bacterial Infections, Length of Stay, Middle Aged, Anti-Bacterial Agents, Cross-Sectional Studies, Infectious Diseases, Carbapenems, Population Surveillance, Anaerobic resistance to metronidazole, Female, Anaerobic bacteria, business, Anaerobic exercise, Anaerobic antimicrobial susceptibility, medicine.drug |
| الوصف: | Background Globally metronidazole and carbapenem resistance in anaerobic organisms is increasing necessitating continuous surveillance to guide selection of empirical treatment. In this study we have determined metronidazole resistance in anaerobes using MIC Evaluator strips (M.I.C.E strips). Carbapenem resistance was evaluated only in metronidazole resistant isolates. Material and methods The study was conducted at the Aga Khan University (AKU) Hospital laboratory, Karachi, Pakistan (2014–2017). Metronidazole and imipenem resistance was evaluated using M.I.C.E strips and minimum inhibitory concentrations (MICs) were interpreted using Clinical Laboratory Standards Institute (CLSI) criteria. Clinical details including demographics, prolonged hospital stay, malignancy, transplant, dialysis, diabetes, site of infection and outcome were analyzed for association with metronidazole resistance. Results Of the 223 clinically significant isolates, 39 (17.5%) were metronidazole resistant (excluding the inherently resistant organisms; for example Cutibacterium species). Imipenem resistance was determined in 29 metronidazole resistant isolates and of these 7 (24.1%) were found to be resistant. Proportion of metronidazole resistant strains was highest amongst Bacteroides species. A significant increase in metronidazole resistance from 12.3% in 2010–2011 to 17.5% in the current study was found. Carbapenem resistance also emerged in the period 2014–2017. Isolates from malignancy and transplant patients showed lower odds of developing metronidazole resistance (0.003(95% CI: 1.7–17.9)). Prolonged hospital stay was not associated with metronidazole resistance (1.1((95% CI: 0.5–2.5)). Conclusion The rising trend of metronidazole resistance and emergence of carbapenem resistance in anaerobic bacteria is alarming. Continued surveillance with strengthening of laboratory capacity regarding anaerobic susceptibility testing is urgently needed in Pakistan. |
| تدمد: | 2047-2994 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d3ed91be43a5fe1e4a0620a958c9b13Test https://doi.org/10.1186/s13756-019-0549-8Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9d3ed91be43a5fe1e4a0620a958c9b13 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20472994 |
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