المؤلفون: Eirin, Maria Emilia, Carignano, Hugo Adrian, Shimizu, Ernesto, Pando, Maria de los Angeles, Zumarraga, Martin Jose, Magnano, Gabriel, Macías, Analía, Garbaccio, Sergio Gabriel, Huertas, Pablo Sebastian, Morsella, Claudia Graciela, Ferrara Muñiz, Ximena, Cataldi, Angel Adrian, Paolicchi, Fernando, Poli, Mario Andres

المصدر: Research in Veterinary Science 130 : 118-125 (June 2020)

مصطلحات موضوعية: Ganado Bovino, Enfermedades de los Animales, Polimorfismo, Tuberculosis, Mycobacterium bovis, Cattle, Animal Diseases, Polymorphism, Mycobacterium bovis Infections, Antigens, Infeccion por Mycobacterium bovis, Antígenos, Tuberculosis Bovina, Bovine Leucocyte Antigen

الوصف: Bovine tuberculosis (bTB) is caused by Mycobacterium bovis and disseminated worldwide. In Argentina, the highest prevalence occurs in dairy areas. BoLA DRB3.2 is related to the adaptive immunity in mycobacterial infections. Genetic polymorphisms of this marker have been associated with resistance or susceptibility to bovine diseases. We evaluated the association between BoLA DRB3.2 polymorphisms and bTB pathology scores in dairy and beef cattle breeds of Argentina. Most bovines exhibited visible lesions compatible with tuberculosis and, furthermore, 150 (85.7%) were also positive by bacteriology. A pathology index showed a variable degree of disease, from 3 to 76 (median pathology score = 9 (IQR: 7–15)). Thirty-five BoLA DRB3.2 alleles were identified with an associated frequency from 16% to 0.3%, distributed 73% (n = 128) in heterozygosis and 27% (n = 47) in homozygosis, with 12 BoLA DRB3.2 alleles (*0101, *1101, *1501, *0201, *2707 *1001, *1002, *1201, *14011, *0501 *0902 and *0701) representing the 74.7% of the population variability. A functional analysis grouped them in 4 out of 5 clusters (A-D), suggesting a functional overlapping. Among the 90 identified genotypes, *1101/*1101, *1101/*1501 and *0101/*0101 were the most frequent (10%, 8.9% and 8.9%, respectively). No association was detected between the pathology scores and a specific DRB3.2 allele (p > .05). Animals infected with M. bovis spoligotype SB0153 showed a significantly higher pathology score than those affected by the spoligotype SB0145 (p = .018). Furthermore, the Aberdeen Angus breed exhibited highest pathological scores (p < .0001), which were associated with disseminated lesion, thus suggesting that the host component could be important to the disease progression. ; Instituto de Biotecnología ; Fil: Eirin, Maria Emilia.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Carignano, Hugo Adrian. Instituto Nacional de ...

وصف الملف: application/pdf

العلاقة: http://hdl.handle.net/20.500.12123/7706Test; https://www.sciencedirect.com/science/article/abs/pii/S0034528819305624Test; https://doi.org/10.1016/j.rvsc.2020.03.001Test

الإتاحة: https://doi.org/20.500.12123/7706Test
https://doi.org/10.1016/j.rvsc.2020.03.001Test
https://hdl.handle.net/20.500.12123/7706Test
https://www.sciencedirect.com/science/article/abs/pii/S0034528819305624Test

المؤلفون: Encinas, Micaela, Marfil, Maria Jimena, Garbaccio, Sergio Gabriel, Barandiaran, Soledad, Huertas, Pablo Sebastian, Morsella, Claudia Graciela, Macias, Analía, Magnano, Gabriel, Zapata, L., Bigi, Fabiana, Cataldi, Angel Adrian, Paolicchi, Fernando, Zumarraga, Martin Jose, Eirin, Maria Emilia

المصدر: Tuberculosis 111: 143–146 (Julio 2018)

مصطلحات موضوعية: Mycobacterium bovis Infections, Polymorphism, Antigens, Isolation Techniques, Infección por Mycobacterium bovis, Polimorfismo, Mycobacterium bovis, Antígenos, Técnicas de Aislamiento, Bovine Tuberculosis, Tuberculosis Bovina

الوصف: ESAT-6, CFP-10 and EspC are virulence factors that have been extensively assayed for bovine and human tuberculosis diagnosis due their potent T-cell inducing activities. While polymorphisms of ESAT-6 and CFP-10 were analyzed, with the description of CFP-10 variants in M. tuberculosis, this fact has not been explored in M. bovis field isolates. The coding sequences of esxA (ESAT-6), esxB (CFP-10) and mb3645c (EspC) from 58 M. bovis strains exhibiting genomic variability (spoligotyping) were analyzed. Two genes –esxA and esxB – remained invariant while mb3645c exhibited one synonymous polymorphism (G to A mutation, position 66bp) in one isolate, compared to M. bovis AF2122/97 reference strain. All isolates exhibited a synonymous nucleotide polymorphism simultaneously (G to A mutation, position 255bp), compared to M. tuberculosis H37Rv reference strain. This study confirms the high conservation for ESAT-6, CFP-10 and EspC in local M. bovis field isolates and reinforce the use of these three antigens in the diagnosis of bovine tuberculosis. Further studies should be performed to globally confirm these findings. ; Instituto de Biotecnología ; Fil: Encinas, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Marfil, María Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina ; Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Huertas, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina ; Fil: Macias, Analía. Universidad Nacional de ...

وصف الملف: application/pdf

العلاقة: info:eu-repograntAgreement/INTA/PNBIO/1131034/AR./Inmunología molecular y genómica funcional aplicadas a interacciones patógeno hospedador de interés pecuario.; info:eu-repograntAgreement/INTA/PNSA/1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.; https://www.sciencedirect.com/science/article/pii/S1472979217304924?via%3DihubTest; http://hdl.handle.net/20.500.12123/3664Test; https://doi.org/10.1016/j.tube.2018.06.007Test

الإتاحة: https://doi.org/20.500.12123/3664Test
https://doi.org/10.1016/j.tube.2018.06.007Test
https://hdl.handle.net/20.500.12123/3664Test
https://www.sciencedirect.com/science/article/pii/S1472979217304924?via%3DihubTest

المؤلفون: Encinas, Micaela, Marfil, Maria Jimena, Garbaccio, Sergio Gabriel, Barandiaran, Soledad, Huertas, Pablo Sebastián, Morsella, C., Macías, A., Magnano, Gabriel Gustavo, Zapata, L., Bigi, Fabiana, Cataldi, Ángel Adrián, Paolicchi, Fernando Alberto, Zumárraga, Martín José, Eirin, Maria Emilia

مصطلحات موضوعية: BOVINE TUBERCULOSIS, CFP-10, ESAT-6, ESPC, FIELD ISOLATES, MYCOBACTERIUM BOVIS, POLYMORPHISM, https://purl.org/becyt/ford/4.3Test, https://purl.org/becyt/ford/4Test

الوصف: ESAT-6, CFP-10 and EspC are virulence factors that have been extensively assayed for bovine and human tuberculosis diagnosis due their potent T-cell inducing activities. While polymorphisms of ESAT-6 and CFP-10 were analyzed, with the description of CFP-10 variants in M. tuberculosis, this fact has not been explored in M. bovis field isolates. The coding sequences of esxA (ESAT-6), esxB (CFP-10) and mb3645c (EspC) from 58 M. bovis strains exhibiting genomic variability (spoligotyping) were analyzed. Two genes –esxA and esxB – remained invariant while mb3645c exhibited one synonymous polymorphism (G to A mutation, position 66bp) in one isolate, compared to M. bovis AF2122/97 reference strain. All isolates exhibited a synonymous nucleotide polymorphism simultaneously (G to A mutation, position 255bp), compared to M. tuberculosis H37Rv reference strain. This study confirms the high conservation for ESAT-6, CFP-10 and EspC in local M. bovis field isolates and reinforce the use of these three antigens in the diagnosis of bovine tuberculosis. Further studies should be performed to globally confirm these findings. ; Fil: Encinas, Micaela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Marfil, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina ; Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina ; Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y ...

وصف الملف: application/pdf

العلاقة: info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1472979217304924Test; http://hdl.handle.net/11336/97548Test; Encinas, Micaela; Marfil, Maria Jimena; Garbaccio, Sergio Gabriel; Barandiaran, Soledad; Huertas, Pablo Sebastián; et al.; Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates; Churchill Livingstone; Tuberculosis (Edinb); 111; 7-2018; 143-146; CONICET Digital; CONICET

الإتاحة: https://doi.org/10.1016/j.tube.2018.06.007Test
http://hdl.handle.net/11336/97548Test