المؤلفون: Jinming Wang, Jifei Yang, Junlong Liu, Hong Yin, Aihong Liu, Guiquan Guan, Xiaoxing Wang, Jianxun Luo, Youquan Li

المصدر: Parasites & Vectors, Vol 14, Iss 1, Pp 1-7 (2021)
Parasites & Vectors

مصطلحات موضوعية: China, Genetic manipulation, Green Fluorescent Proteins, Babesia, Sheep Diseases, Infectious and parasitic diseases, RC109-216, Biology, Transfection, law.invention, Plasmid, Babesia sp. Xinjiang, law, Babesiosis, parasitic diseases, medicine, Animals, Cross-over homologous recombination, Homologous Recombination, Gene, Selectable marker, Polymerase chain reaction, Sheep, Merozoites, Research, fungi, Babesia bovis, biology.organism_classification, medicine.disease, Molecular biology, Infectious Diseases, Parasitology, Stable transfection, Plasmids

الوصف: Background Stable transfection systems have been described in many protozoan parasites, including Plasmodium falciparum, Cryptosporidium parvum, Babesia bovis, Babesia ovata, and Babesia gibsoni. For Babesia sp. Xinjiang (Bxj), which is the causative pathogen of ovine babesiosis and mainly prevails across China, the platform of those techniques remains absent. Genetic manipulation techniques are powerful tools to enhance our knowledge on parasite biology, which may provide potential drug targets and diagnostic markers. Methods We evaluated the inhibition efficiency of blasticidin (BSD) and WR99210 to Bxj. Then, a plasmid was constructed bearing selectable marker BSD, green fluorescent protein (GFP) gene, and rhoptry-associated protein-1 3′ terminator region (rap 3′ TR). The plasmid was integrated into the elongation factor-1 alpha (ef-1α) site of Bxj genome by cross-over homologous recombination technique. Twenty μg of plasmid was transfected into Bxj merozoites. Subsequently, drug selection was performed 24 h after transfection to generate transfected parasites. Results Transfected parasite lines, Bxj-c1, Bxj-c2, and Bxj-c3, were successfully obtained after transfection, drug selection, and colonization. Exogenous genes were integrated into the Bxj genome, which were confirmed by PCR amplification and sequencing. In addition, results of western blot (WB) and indirect immunofluorescence assay (IFA) revealed that GFP-BSD had expressed for 11 months. Conclusions In our present study, stable transfection system for Bxj was successfully developed. We anticipate that this platform will greatly facilitate basic research of Bxj. Graphical abstract

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1315d68494604195019d435e9c0fe8a5Test
https://doaj.org/article/b00d31d1baea4290ac2cccdc7964d4e1Test

المؤلفون: Yang Wang, Junfei Dai, Yongsheng Liu, Jifei Yang, Qian Hou, Yunwen Ou, Yaozhong Ding, Bing Ma, Haotai Chen, MiaoMiao Li, Yuefeng Sun, Haixue Zheng, Keshan Zhang, Ashenafi Kiros Wubshet, Alexei D. Zaberezhny, Taras I. Aliper, Kazimierz Tarasiuk, Zygmunt Pejsak, Zhijie Liu, Yongguang Zhang, Jie Zhang

المصدر: Frontiers in Microbiology, Vol 12 (2021)
Frontiers in Microbiology

مصطلحات موضوعية: Microbiology (medical), genetic structures, 040301 veterinary sciences, Loop-mediated isothermal amplification, African swine fever virus, Microbiology, Virus, law.invention, 0403 veterinary science, 03 medical and health sciences, law, Asfarviridae, one-step, diagnostics, visual LAMP, neutral red, Polymerase chain reaction, Original Research, 030304 developmental biology, 0303 health sciences, biology, 04 agricultural and veterinary sciences, biology.organism_classification, Monitoring program, Virology, penside, QR1-502, Capsid, Recombinant DNA, ASFV

الوصف: African swine fever (ASF) has caused huge economic losses to the swine industry worldwide. Since there is no commercial ASF vaccine available, an early diagnosis is extremely important to prevent and control the disease. In this study, ASF virus (ASFV) capsid protein-encoding gene (p72) was selected and used to design primers for establishing a one-step visual loop-mediated isothermal amplification (LAMP) assay with neutral red, a pH-sensitive dye, as the color shift indicator. Neutral red exhibited a sharp contrast of color change from faint orange (negative) to pink (positive) during LAMP for detection of ASFV. The designed primer set targeting highly conserved region of the p72 gene was highly specific to ASFV and showed no cross-reactivity with other swine viruses. The detection limit for the one-step visual LAMP developed was 10 copies/reaction based on the recombinant plasmid containing the p72 gene of ASFV. More importantly, the developed one-step visual LAMP showed high consistency with the results of the real-time polymerase chain reaction (qPCR) method recommended by World Organization for Animal Health (OIE). Furthermore, the results demonstrate that the colorimetric detection with this LAMP assay could be directly applied for the whole blood and serum samples without requiring genome extraction. Based on our results, the developed one-step visual LAMP assay is a promising penside diagnostic tool for development of early and cost-effective ASF monitoring program that would greatly contribute to the prevention and control of ASF.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b13df3e9b07013b7efe2705077c52274Test
https://www.frontiersin.org/articles/10.3389/fmicb.2021.609821/fullTest

المؤلفون: Xu Jianlin, Youquan Li, Xiaoxing Wang, Hong Yin, Jifei Yang, Zhijie Liu, Jianxun Luo, Zhi Li, Jinming Wang, Xuan Li, Quanjia Xiang, Junlong Liu, Guiquan Guan

المصدر: Ticks and Tick-borne Diseases. 10:528-532

مصطلحات موضوعية: 0301 basic medicine, China, Veterinary medicine, Genotype, Babesia caballi, animal diseases, 030231 tropical medicine, ved/biology.organism_classification_rank.species, Babesia, Enzyme-Linked Immunosorbent Assay, Biology, Polymerase Chain Reaction, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Babesiosis, Theileria, parasitic diseases, RNA, Ribosomal, 18S, Animals, Horses, Phylogeny, Genetic diversity, Molecular epidemiology, Phylogenetic tree, ved/biology, Genetic Variation, High-Throughput Nucleotide Sequencing, DNA, Protozoan, bacterial infections and mycoses, Equine piroplasmosis, Theileriasis, 030104 developmental biology, Infectious Diseases, Theileria equi, Tick-Borne Diseases, Insect Science, Horse Diseases, Parasitology, Nested polymerase chain reaction

الوصف: Equine piroplasmosis, caused by Theileria equi and Babesia caballi, is an economically important tick-borne disease worldwide. In the current study, 242 blood samples were randomly collected from horses in Zhangye city of Gansu province, China. The presence and genetic diversity of piroplasms were evaluated with a nested PCR assay, gene sequencing and phylogenetic analysis. The results showed that seventy-five (31.0%) samples were positive for piroplasms. Sequences analysis showed that seventy-three (30.2%) were positive for T. equi, and seven (2.9%) for B. caballi, five of which (2.1%) were infected with T. equi and B. caballi. Phylogenetic analysis revealed two T. equi genotypes (C and E) and one B. caballi genotype (A). The molecular epidemiological and genetic diversity results provide important epidemiological data for control of equine piroplasmosis caused by T. equi and B. caballi in China.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::990d1f66ad5ec09f4417783a3ebf713cTest
https://doi.org/10.1016/j.ttbdis.2019.01.003Test

المؤلفون: Guiquan Guan, Jinming Wang, Jifei Yang, Xiaoxing Wang, Hao Sun, Hong Yin, Zhaoyong Lv, Youquan Li, Junlong Liu, Jianxun Luo, Aihong Liu, Shandian Gao

المصدر: Pathogens, Vol 9, Iss 473, p 473 (2020)
Pathogens
Volume 9
Issue 6

مصطلحات موضوعية: Microbiology (medical), Genetics, Genetic diversity, China, General Immunology and Microbiology, biology, Phylogenetic tree, lcsh:R, lcsh:Medicine, Babesia bovis, genetic diversity, biology.organism_classification, law.invention, Infectious Diseases, Antigen, law, parasitic diseases, Immunology and Allergy, Parasite hosting, merozoite surface antigen, Molecular Biology, Nested polymerase chain reaction, Gene, Polymerase chain reaction

الوصف: The apicomplexan parasite Babesia bovis is a tick-borne intracellular hemoprotozoan parasite that is widespread across China. Genetic diversity is an important strategy used by parasites to escape the immune responses of their hosts. In our present study, 575 blood samples, collected from cattle in 10 provinces, were initially screened using a nested PCR (polymerase chain reaction) for detection of B. bovis infection. To perform genetic diversity analyses, positive samples were further amplified to obtain sequences of three B. bovis merozoite surface antigen genes (MSA-1, MSA-2b, MSA-2c). The results of the nested PCR approach showed that an average of 8.9% (51/575) of cattle were positive for B. bovis infection. Phylogenetic analyses of the predicted amino acid sequences revealed that unique antigen variants were formed only by Chinese isolates. Our findings provide vital information for understanding the genetic diversity of B. bovis in China.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54edd07498bdb9f330aa21a41f0b9f43Test
https://www.mdpi.com/2076-0817/9/6/473Test

المؤلفون: Youquan Li, Jianxun Luo, Hong Yin, Zhijie Liu, Yuping Pan, Hongxi Zhao, Qingli Niu, Bintao Zhai, Jifei Yang

المصدر: Infection, Genetics and Evolution

مصطلحات موضوعية: 0301 basic medicine, Microbiology (medical), Veterinary medicine, endocrine system, China, Camelus, relapsing fever, 030106 microbiology, Camelus bactrianus, Microbiology, Polymerase Chain Reaction, Article, Animal Diseases, 03 medical and health sciences, Lyme disease, Borrelia, Genetics, medicine, Animals, Bactrian camel, Geography, Medical, Molecular Biology, Ecology, Evolution, Behavior and Systematics, Phylogeny, Lyme Disease, Borrelia spp, biology, Genes, rRNA, Ribosomal RNA, biology.organism_classification, medicine.disease, 16S ribosomal RNA, bacterial infections and mycoses, 030104 developmental biology, Infectious Diseases, Borrelia Infections, Nested polymerase chain reaction, Nested PCR

الوصف: Comprehensive epidemiological surveys for Lyme disease have not been conducted for the Bactrian camel in China. In this study, a total of 138 blood specimens collected from Bactrian camels from Zhangye City in Gansu Province and Yili and Aksu in Xinjiang Province, China, were examined for the presence of Borrelia spp. Species-specificity nested PCR based on the 5S-23S rRNA, OspA, flaB and 16S rRNA genes revealed that the total positive rate of Borrelia spp. was 3.6% (5/138, 95% CI = 0.2–17.9). These results were confirmed by sequence analysis of the positive PCR products or positive colonies. This is the first report of Borrelia pathogens in camels in China. Two Borrelia species that cause Lyme disease and one that causes relapsing fever were identified in the camel blood samples by sequencing. The findings of this study indicate that the Bactrian camel may serve as a potential natural host of Lyme disease and/or relapsing fever in China.
Highlights • This is the first report of Borrelia pathogens using molecular tools in Bactrian camel. • Bactrian camel may play as potential natural host of Lyme disease in China. • The results revealed that the prevalence of Borrelia spp. were 3.6% based on sequences of four genes. • A novel Borrelia genespecies belong to Relapsing fever Borrelia spp. was found.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3dabe04a614aa93304a0b66320c08fefTest
http://europepmc.org/articles/PMC7106200Test

المؤلفون: Jifei Yang, Jianxun Luo, Zhijie Liu, Guiquan Guan, Guangyuan Liu, Qingli Niu, Hong Yin, Rong Han

المصدر: Ticks and Tick-borne Diseases. 9:82-85

مصطلحات موضوعية: DNA, Bacterial, 0301 basic medicine, Anaplasmosis, China, Veterinary medicine, Anaplasma, Genotype, Anaplasma bovis, animal diseases, Sheep Diseases, Polymerase Chain Reaction, Microbiology, 03 medical and health sciences, Bacterial Proteins, Species Specificity, RNA, Ribosomal, 16S, parasitic diseases, Prevalence, medicine, Animals, Ovis, Phylogeny, Sheep, biology, Anaplasma ovis, Sequence Analysis, DNA, bacterial infections and mycoses, biology.organism_classification, medicine.disease, Anaplasma phagocytophilum, 030104 developmental biology, Infectious Diseases, Insect Science, Coinfection, bacteria, Parasitology, Capra

الوصف: The members of the genus Anaplasma are important tick-borne rickettsial bacteria of veterinary and public health significance. Domestic ruminants are important reservoir hosts for several Anaplasma species. In this study, the occurrence of Anaplasma spp. was investigated by PCR in domestic small ruminants from Gansu, northwestern China. The results showed a high prevalence of Anaplasma spp. (46.2%, 201/435) in sheep. The average infection rates were 5.7%, 24.4%, 28.0% and 18.2% for A. ovis, A. bovis, A. phagocytophilum and A. capra, respectively. Coinfection of different Anaplasma species occurred in 96 (22.1%) sheep. The infections of Anaplasma species in sheep were confirmed by sequencing of msp4, 16S rRNA and gltA genes. Sequence analysis revealed a novel A. capra genotype in sheep that was distinct from the isolates identified from patients in northeastern China. This study gives the first insight of presence of four distinct Anaplasma species with veterinary and medical significance in sheep in northwestern China.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55aeab03780b03040862916e4bb4bd4fTest
https://doi.org/10.1016/j.ttbdis.2017.10.005Test

المؤلفون: Zhijie Liu, Ze Chen, Peifa Yu, Hong Yin, Jianxun Luo, Jifei Yang, Qingli Niu, Guangyuan Liu, Mirza Omar Abdallah

المصدر: Experimental and Applied Acarology. 73:269-281

مصطلحات موضوعية: DNA, Bacterial, 0301 basic medicine, China, Veterinary medicine, Entomology, Ixodidae, Anaplasma bovis, 030106 microbiology, Cattle Diseases, Polymerase Chain Reaction, 03 medical and health sciences, RNA, Ribosomal, 16S, parasitic diseases, Animals, Anaplasma, Borrelia burgdorferi, Pathogen, Rickettsia massiliae, Ecology, biology, Bacterial Infections, Sequence Analysis, DNA, General Medicine, DNA, Protozoan, bacterial infections and mycoses, biology.organism_classification, Virology, Tick Infestations, 030104 developmental biology, Tick-Borne Diseases, Animal ecology, Insect Science, bacteria, Cattle, Female, Hyalomma, Bacterial Outer Membrane Proteins

الوصف: Although tick-borne pathogens have been widely reported in ticks in China, there is little information available on the prevalence of information in Hyalomma ticks from cattle. This study aims to determine the occurrence of pathogens in Hyalomma anatolicum collected from cattle in Xinjiang Uygur Autonomous Region, China, by PCR, sequencing and phylogenetic analysis. Borrelia burgdorferi s.s., Rickettsia massiliae and Anaplasma bovis were identified, whereas DNA of Ehrlichia species and an Anaplasma platys-like pathogen were also detected. Our findings highlight the risk of infection of animals and humans with these pathogens in north-western China.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::efc2359d705aa7bcff02a8e0889ef648Test
https://doi.org/10.1007/s10493-017-0162-6Test

المؤلفون: Jifei Yang, Jianxun Luo, Youquan Li, Zhijie Liu, Xiaoxing Wang, Hong Yin, Jinming Wang, Guiquan Guan, Junlong Liu

المصدر: Parasitology international. 71

مصطلحات موضوعية: Male, China, Genotype, Babesia, Biology, Polymerase Chain Reaction, 18S ribosomal RNA, Dogs, Babesiosis, RNA, Ribosomal, 18S, Animals, Dog Diseases, Genetic diversity, Genetic Variation, High-Throughput Nucleotide Sequencing, Pets, DNA, Protozoan, biology.organism_classification, Virology, Infectious Diseases, Canis, GenBank, Babesia canis, Parasitology, Female, Nested polymerase chain reaction

الوصف: Canine babesiosis is an important tick-borne disease caused by Babesia spp. A total of 130 blood samples were randomly collected from pet dogs in Zhengzhou city, Henan Province, China, and screened for the presence of piroplasms with nested PCR and gene sequencing targeting the 18S rRNA gene. On the basis of blast analysis of the 18S rRNA gene sequences, our results revealed that seven dogs (5.4%) were infected with Babesia canis canis. The sequences were compared with those in GenBank, and alignments showed that all B. canis canis isolates belonged to genotype B. This is the first report of B. canis canis infection in dogs in China.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cbd2a3603a8d267a5c1fd1c5eabadb53Test
https://pubmed.ncbi.nlm.nih.gov/30885796Test

المؤلفون: Youquan Li, Gege Qin, Zhijie Liu, Junlong Liu, Hong Yin, Guiquan Guan, Guangyuan Liu, Jifei Yang, Lin Zhang, Jianxun Luo

المصدر: Parasitology Research. 115:2647-2652

مصطلحات موضوعية: 0301 basic medicine, China, Veterinary medicine, 030231 tropical medicine, Theileria luwenshuni, Tibet, Polymerase Chain Reaction, law.invention, 03 medical and health sciences, 0302 clinical medicine, law, Theileria, parasitic diseases, Theileria sinensis, Animals, Polymerase chain reaction, geography, Theileria sp, Plateau, geography.geographical_feature_category, General Veterinary, biology, business.industry, General Medicine, 030108 mycology & parasitology, biology.organism_classification, Theileriasis, Infectious Diseases, Insect Science, Theileria orientalis, Cattle, Parasitology, Livestock, business

الوصف: Theileriosis continues to threaten the livestock industry worldwide, but comprehensive epidemiological surveys for this disease have not been conducted in the Tibet Plateau Region, China. In this study, we screened 154 cattle blood samples from the Tibet Plateau Region (Lhasa, Lhoka, and Tianzhu), China, for detection of Theileria pathogens by polymerase chain reaction (PCR) with species-specific primers. The results revealed that the prevalence was 6.9 % (2/29) for Theileria orientalis and 27.6 % (8/29) for Theileria sinensis in Lhasa, 0 % (0/30) for T. orientalis and 26.7 % (8/30) for T. sinensis in Lhoka, and 0 % (0/95) for T. orientalis and 30.5 % (29/95) for T. sinensis in Tianzhu. Interestingly, Theileria luwenshuni, which was a previously reported pathogenic Theileria sp. in sheep and goats, was detected in blood samples from cattle and yaks for the first time, with a prevalence of 10 % (3/30) in Lhoka and 1.1 % (1/95) in Tianzhu. No other Theileria sp. was detected in these samples. T. sinensis and T. orientalis infections were detected in cattle and yaks, and T. luwenshuni was discovered for the first time in cattle and yaks in the Tibet Plateau Region, China.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ef671f36a826cd31fac65a39d30e6baTest
https://doi.org/10.1007/s00436-016-5011-8Test

المؤلفون: Youquan Li, Junlong Liu, Jianxun Luo, Zhijie Liu, Qiaoyun Ren, Jinming Wang, Jianlin Xu, Guiquan Guan, Xiaoxing Wang, Aihong Liu, Hong Yin, Jifei Yang

المصدر: Parasitology research. 117(12)

مصطلحات موضوعية: 0301 basic medicine, medicine.medical_specialty, China, Genotype, 030231 tropical medicine, Protozoan Proteins, Zoology, Cattle Diseases, Antigens, Protozoan, Biology, Polymerase Chain Reaction, 03 medical and health sciences, 0302 clinical medicine, Medical microbiology, Theileria, parasitic diseases, medicine, Animals, Gene, Phylogeny, Genetic diversity, Molecular Epidemiology, General Veterinary, Phylogenetic tree, Molecular epidemiology, Genetic Variation, Membrane Proteins, General Medicine, 030108 mycology & parasitology, Theileriasis, Infectious Diseases, Tick-Borne Diseases, Insect Science, Theileria orientalis, Parasitology, Cattle, Surface protein, Genome, Protozoan

الوصف: The apicomplexan parasite Theileria orientalis is a tick-borne intracellular protozoan parasite that is widely distributed throughout China. It causes bovine theileriosis in infected cattle, which results in huge economic losses to the cattle industry. In this study, the infection status of T. orientalis was determined in 260 blood samples from cattle from seven provinces across China. Results of a major piroplasm surface protein (MPSP)-PCR assay revealed that an average of 36.5% (95/260) of cattle was positive for T. orientalis infection. Based on the MPSP gene sequences, phylogenetic analysis revealed that these isolates of T. orientalis comprised of eight MPSP types, 1, 2, 3, 4, 5, 7, N1, and N2. This is the first report of new T. orientalis MPSP genotypes N1 and N2 in cattle in China.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c1d97d4fa23ab0959dced885ac41f5aTest
https://pubmed.ncbi.nlm.nih.gov/30056555Test