المؤلفون: Totsline, Noah, Kniel, Kalmia E., Sabagyanam, Chandran, Bais, Harsh P.

مصطلحات موضوعية: plant physiology, plant signalling, plant stress responses, stomata

الوصف: This article was originally published in Scientific Reports. The version of record is available at: https://doi.org/10.1038/s41598-024-51573-yTest. © The Author(s) 2024. This article was featured in a UDaily article on 1/17/2024 available at: https://www.udel.edu/udaily/2024/january/harsh-bais-kali-kniel-noah-totsline-spacegrown-plants-foodborne-infectionsTest/ ; As human spaceflight increases in duration, cultivation of crops in spaceflight is crucial to protecting human health under microgravity and elevated oxidative stress. Foodborne pathogens (e.g., Salmonella enterica) carried by leafy green vegetables are a significant cause of human disease. Our previous work showed that Salmonella enterica serovar Typhimurium suppresses defensive closure of foliar stomata in lettuce (Lactuca sativa L.) to ingress interior tissues of leaves. While there are no reported occurrences of foodborne disease in spaceflight to date, known foodborne pathogens persist aboard the International Space Station and space-grown lettuce has been colonized by a diverse microbiome including bacterial genera known to contain human pathogens. Interactions between leafy green vegetables and human bacterial pathogens under microgravity conditions present in spaceflight are unknown. Additionally, stomatal dynamics under microgravity conditions need further elucidation. Here, we employ a slow-rotating 2-D clinostat to simulate microgravity upon in-vitro lettuce plants following a foliar inoculation with S. enterica Typhimurium and use confocal microscopy to measure stomatal width in fixed leaf tissue. Our results reveal significant differences in average stomatal aperture width between an unrotated vertical control, plants rotated at 2 revolutions per minute (2 RPM), and 4 RPM, with and without the presence of S. typhimurium. Interestingly, we found stomatal aperture width in the presence of S. typhimurium to be increased under rotation as compared to unrotated inoculated plants. Using confocal Z-stacking, we observed greater average depth of stomatal ...

وصف الملف: application/pdf

العلاقة: Totsline, N., Kniel, K.E., Sabagyanam, C. et al. Simulated microgravity facilitates stomatal ingression by Salmonella in lettuce and suppresses a biocontrol agent. Sci Rep 14, 898 (2024). https://doi.org/10.1038/s41598-024-51573-yTest; https://udspace.udel.edu/handle/19716/33842Test

الإتاحة: https://doi.org/10.1038/s41598-024-51573-yTest
https://udspace.udel.edu/handle/19716/33842Test

المؤلفون: Tachibana, Ryo, Abe, Susumu, Marugami, Momo, Yamagami, Ayumi, Akema, Rino, Ohashi, Takao, Nishida, Kaisei, Nosaki, Shohei, Miyakawa, Takuya, Tanokura, Masaru, Kim, Jong-Myong, Seki, Motoaki, Inaba, Takehito, Matsui, Minami, Ifuku, Kentaro, Kushiro, Tetsuo, Asami, Tadao, Nakano, Takeshi

المساهمون: 立花, 諒, 阿部, 晋, 丸上, 萌々, 山上, あゆみ, 明間, 莉乃, 大橋, 隆生, 西田, 快世, 野﨑, 翔平, 宮川, 拓也, 田之倉, 優, 金, 鍾明, 関, 原明, 稲葉, 丈人, 松井, 南, 伊福, 健太郎, 久城, 哲夫, 浅見, 忠男, 中野, 雄司, 00599615, 50596559, 30281653

مصطلحات موضوعية: Brassinosteroid, Plant development, Plant signalling

الوصف: 葉緑体の発達を適正に制御する新しい因子を発見. 京都大学プレスリリース. 2024-01-23. ; Chloroplast development adapts to the environment for performing suitable photosynthesis. Brassinosteroids (BRs), plant steroid hormones, have crucial effects on not only plant growth but also chloroplast development. However, the detailed molecular mechanisms of BR signaling in chloroplast development remain unclear. Here, we identify a regulator of chloroplast development, BPG4, involved in light and BR signaling. BPG4 interacts with GOLDEN2-LIKE (GLK) transcription factors that promote the expression of photosynthesis-associated nuclear genes (PhANGs), and suppresses their activities, thereby causing a decrease in the amounts of chlorophylls and the size of light-harvesting complexes. BPG4 expression is induced by BR deficiency and light, and is regulated by the circadian rhythm. BPG4 deficiency causes increased reactive oxygen species (ROS) generation and damage to photosynthetic activity under excessive high-light conditions. Our findings suggest that BPG4 acts as a chloroplast homeostasis factor by fine-tuning the expression of PhANGs, optimizing chloroplast development, and avoiding ROS generation.

العلاقة: https://www.kyoto-u.ac.jp/ja/research-news/2024-01-23-0Test; http://hdl.handle.net/2433/286758Test; Nature Communications; 15; 370

الإتاحة: http://hdl.handle.net/2433/286758Test

المؤلفون: Piovesana, Maiara

المساهمون: Kumar, Vinod, Matthes, Michaela, Kurup, Smita, Napier, Johnathan, Talbot, Nicholas

مصطلحات موضوعية: Arabidopsis, Receptor-like kinase, Cysteine-rich receptor-like kinase, Arabidopsis thaliana, Plant signalling

الوصف: Receptor-like kinases (RLKs) comprise a large superfamily of proteins in plant genomes, and play essential roles in plant growth, development and response to biotic and abiotic stresses. The Cysteine-Rich Receptor-Like Kinases (CRKs) comprise one of the largest subfamilies of RLKs with over 40 members in Arabidopsis thaliana, and although a few members of the family have been initially characterised, their precise biological functions remain largely unknown. This thesis reports the characterisation of a novel gain-of-function allele of Cysteine-Rich Receptor-Like Kinase 10 (CRK10) in A. thaliana which was isolated from a chemical mutagenesis screen. This mutation causes the substitution of alanine 397 with a threonine residue in subdomain III / αC-helix of the kinase domain of CRK10, and this novel allele has been accordingly registered as crk10-A397T with the Arabidopsis community database. The crk10- A397T mutant is a dwarf, and anatomical characterisation unveiled severely collapsed xylem vessels in the root and hypocotyl of the plant. Reporter lines suggested CRK10 is expressed in close association to vascular tissues, and a translational fusion with the fluorescent protein mCherry indicates that CRK10 is a plasma membrane-bound protein. Analysis of the recombinant WT and crk10- A397T versions of the cytoplasmic kinase domain of CRK10 demonstrated their auto-phosphorylation activity, and liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis concluded that Thr397 acts as an additional auto-phosphorylation site in situ. Furthermore, an RNA-seq experiment revealed the constitutive induction of defence-related genes in the transcriptome of crk10- A397T mutant hypocotyls, including genes involved in the signalling pathways of the stress hormones salicylic acid (SA) and abscisic acid (ABA). Analysis of the composition of cell walls in the crk10-A397T mutant hypocotyls revealed extensive differences compared to the WT, an indication of cell wall remodelling mechanisms that are likely associated with the collapse of xylem vessels in this organ. Bioassays with the soil-borne vascular pathogen Fusarium oxysporum revealed that crk10-A397T mutant has a greater probability of survival to infection compared to WT plants. Analysis of genetic crosses demonstrated that key components of SA signalling pathways are required for the disease resistance phenotype of the crk10-A397T mutant.

الوصول الحر: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.846030Test

المؤلفون: Davide Savy, Vincenza Cozzolino, Giovanni Vinci, Mariavittoria Verrillo, Antonietta Aliberti, Albino Maggio, Amalia Barone, Alessandro Piccolo

المصدر: Chemical and Biological Technologies in Agriculture, Vol 9, Iss 1, Pp 1-13 (2022)

مصطلحات موضوعية: Organic amendments, Salinity, Stress tolerance, Gas chromatography mass spectrometry (GC–MS), Soluble sugars and amino acids, Plant signalling, Agriculture

الوصف: Abstract Background Salinity is one of the major threats for crop growth and yield and its rate of expansion is expected to increase. We conducted a pot experiment to evaluate and compare the effect of a green compost addition and mineral fertilisation, on growth, nutrition and metabolites of tomato plants, exposed to increasing doses of NaCl. Results Although the development of stressed plants was lower than the corresponding controls, compost-treated plants performed better than mineral-amended plants watered with the same amount of salt. The different plant growth was related to an increased nutritional status. Namely, compost-treated plants showed a larger content of macro- and micronutrients, and a greater accumulation of osmoprotectants, such as soluble sugars and amino acids. Moreover, compost-treated plants showed a larger content of metabolites involved in modulating the response to salt stress, such as molecules related to energy transfer in plants and precursors of Reactive Oxygen Species scavenging compounds. Overall, the better performance of compost-added plants may be attributed to a greater availability of the organic forms of nutrients and to the positive bioactivity of compost-derived humic substances. Conclusions Compost application efficiently mitigated salt stress in tomato plants in respect to mineral fertilisation. This alleviating role was associated to the induction of a more efficient metabolic response that increased accumulation of metabolites involved in modulating the salinity stress. Therefore, fertilising with composted agricultural residue may represent a convenient alternative to mineral fertilisers to grow tomato plants in the presence of salt stress. Graphical Abstract

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2196-5641Test

الوصول الحر: https://doaj.org/article/f9ede6807dad4cec82f3e62592f0c70aTest

المؤلفون: Jędrzej Dobrogojski, Van Hai Nguyen, Joanna Kowalska, Sławomir Borek, Małgorzata Pietrowska-Borek

المصدر: International Journal of Molecular Sciences, Vol 24, Iss 23, p 16688 (2023)

مصطلحات موضوعية: abscisic acid, diadenosine tetraphosphate (Ap4A), dicytidine tetraphosphate (Cp4C), dinucleoside polyphosphates (NpnNs), extracellular ATP (eATP), plant signalling, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: Dinucleoside polyphosphates (NpnNs) are considered novel signalling molecules involved in the induction of plant defence mechanisms. However, NpnN signal recognition and transduction are still enigmatic. Therefore, the aim of our research was the identification of the NpnN receptor and signal transduction pathways evoked by these nucleotides. Earlier, we proved that purine and pyrimidine NpnNs differentially affect the phenylpropanoid pathway in Vitis vinifera suspension-cultured cells. Here, we report, for the first time, that both diadenosine tetraphosphate (Ap4A) and dicytidine tetraphosphate (Cp4C)-induced stomatal closure in Arabidopsis thaliana. Moreover, we showed that plasma membrane purinoreceptor P2K1/DORN1 (does not respond to nucleotide 1) is essential for Ap4A-induced stomata movements but not for Cp4C. Wild-type Col-0 and the dorn1-3 A. thaliana knockout mutant were used. Examination of the leaf epidermis dorn1-3 mutant provided evidence that P2K1/DORN1 is a part of the signal transduction pathway in stomatal closure evoked by extracellular Ap4A but not by Cp4C. Reactive oxygen species (ROS) are involved in signal transduction caused by Ap4A and Cp4C, leading to stomatal closure. Ap4A induced and Cp4C suppressed the transcriptional response in wild-type plants. Moreover, in dorn1-3 leaves, the effect of Ap4A on gene expression was impaired. The interaction between P2K1/DORN1 and Ap4A leads to changes in the transcription of signalling hubs in signal transduction pathways.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1422-0067/24/23/16688Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/986838f5febe43ef936ceb90aaa383a5Test

المؤلفون: Cui, Jinying, Nishide, Noriko, Mashiguchi, Kiyoshi, Kuroha, Kana, Miya, Masayuki, Sugimoto, Kazuhiko, Itoh, Jun-Ichi, Yamaguchi, Shinjiro, Izawa, Takeshi

المساهمون: サイ, キンエイ, 西出, 典子, 増口, 潔, 黒羽, 加奈, 味谷, 雅之, 杉本, 和彦, 伊藤, 純一, 山口, 信次郎, 井澤, 毅

مصطلحات موضوعية: Plant morphogenesis, Plant signalling, Strigolactone

الوصف: 低施肥でも穂数が減らず、収量を確保できるイネを開発 --ゲノム編集技術で、SDGs時代の新しいイネ遺伝資源を創成--. 京都大学プレスリリース. 2023-06-13. ; Fertilization controls various aspects of cereal growth such as tiller number, leaf size, and panicle size. However, despite such benefits, global chemical fertilizer use must be reduced to achieve sustainable agriculture. Here, based on field transcriptome data from leaf samples collected during rice cultivation, we identify fertilizer responsive genes and focus on Os1900, a gene orthologous to Arabidopsis thaliana MAX1, which is involved in strigolactone biosynthesis. Elaborate genetic and biochemical analyses using CRISPR/Cas9 mutants reveal that Os1900 together with another MAX1-like gene, Os5100, play a critical role in controlling the conversion of carlactone into carlactonoic acid during strigolactone biosynthesis and tillering in rice. Detailed analyses of a series of Os1900 promoter deletion mutations suggest that fertilization controls tiller number in rice through transcriptional regulation of Os1900, and that a few promoter mutations alone can increase tiller numbers and grain yields even under minor-fertilizer conditions, whereas a single defective os1900 mutation does not increase tillers under normal fertilizer condition. Such Os1900 promoter mutations have potential uses in breeding programs for sustainable rice production.

العلاقة: https://www.kyoto-u.ac.jp/ja/research-news/2023-06-13Test; http://hdl.handle.net/2433/283305Test; Nature Communications; 14; 3191

الإتاحة: http://hdl.handle.net/2433/283305Test

المؤلفون: Taleski, Michael, Chapman, Kelly, Novák, Ondřej, Schmülling, Thomas, Frank, Manuel, Djordjevic, Michael A.

مصطلحات موضوعية: Cytokinin, Plant development, Plant signalling, ddc:570

الوصف: C-TERMINALLY ENCODED PEPTIDE (CEP) and cytokinin hormones act over short and long distances to control plant responses to environmental cues. CEP and cytokinin pathway mutants share phenotypes, however, it is not known if these pathways intersect. We show that CEP and cytokinin signalling converge on CEP DOWNSTREAM (CEPD) glutaredoxins to inhibit primary root growth. CEP inhibition of root growth was impaired in mutants defective in trans-zeatin (tZ)-type cytokinin biosynthesis, transport, perception, and output. Concordantly, mutants affected in CEP RECEPTOR 1 showed reduced root growth inhibition in response to tZ, and altered levels of tZ-type cytokinins. Grafting and organ-specific hormone treatments showed that tZ-mediated root growth inhibition involved CEPD activity in roots. By contrast, root growth inhibition by CEP depended on shoot CEPD function. The results demonstrate that CEP and cytokinin pathways intersect, and utilise signalling circuits in separate organs involving common glutaredoxin genes to coordinate root growth.

وصف الملف: 11 Seiten; application/pdf

العلاقة: https://refubium.fu-berlin.de/handle/fub188/43209Test; http://dx.doi.org/10.17169/refubium-42925Test

الإتاحة: https://doi.org/10.17169/refubium-42925Test
https://doi.org/10.1038/s41467-023-37282-6Test
https://refubium.fu-berlin.de/handle/fub188/43209Test

المؤلفون: Vainonen, Julia P., Gossens, Richard, Krasensky-Wrzaczek, Julia, De Masi, Raffaella, Danciu, Iulia, Puukko, Tuomas, Battchikova, Natalia, Jonak, Claudia, Wirthmueller, Lennart, Wrzaczek, Michael

مصطلحات موضوعية: Plant molecular biology, Plant signalling, Photoregulatory Protein Kinases, ddc:580

الوصف: Poly(ADP-ribosyl)ation (PARylation) is a reversible post-translational protein modification that has profound regulatory functions in metabolism, development and immunity, and is conserved throughout the eukaryotic lineage. Contrary to metazoa, many components and mechanistic details of PARylation have remained unidentified in plants. Here we present the transcriptional co-regulator RADICAL-INDUCED CELL DEATH1 (RCD1) as a plant PAR-reader. RCD1 is a multidomain protein with intrinsically disordered regions (IDRs) separating its domains. We have reported earlier that RCD1 regulates plant development and stress-tolerance by interacting with numerous transcription factors (TFs) through its C-terminal RST domain. This study suggests that the N-terminal WWE and PARP-like domains, as well as the connecting IDR play an important regulatory role for RCD1 function. We show that RCD1 binds PAR in vitro via its WWE domain and that PAR-binding determines RCD1 localization to nuclear bodies (NBs) in vivo. Additionally, we found that RCD1 function and stability is controlled by Photoregulatory Protein Kinases (PPKs). PPKs localize with RCD1 in NBs and phosphorylate RCD1 at multiple sites affecting its stability. This work proposes a mechanism for negative transcriptional regulation in plants, in which RCD1 localizes to NBs, binds TFs with its RST domain and is degraded after phosphorylation by PPKs.

وصف الملف: 10 Seiten; application/pdf

العلاقة: https://refubium.fu-berlin.de/handle/fub188/39769Test; http://dx.doi.org/10.17169/refubium-39487Test

الإتاحة: https://doi.org/10.17169/refubium-39487Test
https://doi.org/10.1038/s42003-023-04794-2Test
https://refubium.fu-berlin.de/handle/fub188/39769Test

المؤلفون: Dobrogojski, Jędrzej, Nguyen, Van Hai, Kowalska, Joanna, Borek, Sławomir, Pietrowska-Borek, Małgorzata

المساهمون: Department of Biochemistry and Biotechnology, Faculty of Agriculture, Horticulture and Bioengineering, Poznań University of Life Sciences, Poznań, Poland, Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Warsaw, Poland, Department of Plant Physiology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

مصطلحات موضوعية: abscisic acid, diadenosine tetraphosphate (Ap4A), dicytidine tetraphosphate (Cp4C), dinucleoside polyphosphates (NpnNs), extracellular ATP (eATP), plant signalling, reactive oxygen species (ROS), uncommon nucleotides

الوصف: Dinucleoside polyphosphates (NpnNs) are considered novel signalling molecules involved in the induction of plant defence mechanisms. However, NpnN signal recognition and transduction are still enigmatic. Therefore, the aim of our research was the identification of the NpnN receptor and signal transduction pathways evoked by these nucleotides. Earlier, we proved that purine and pyrimidine NpnNs differentially affect the phenylpropanoid pathway in Vitis vinifera suspension cultured cells. Here, we report, for the first time, that both diadenosine tetraphosphate (Ap4A) and dicytidine tetraphosphate (Cp4C)-induced stomatal closure in Arabidopsis thaliana. Moreover, we showed that plasma membrane purinoreceptor P2K1/DORN1 (does not respond to nucleotide 1) is essential for Ap4A-induced stomata movements but not for Cp4C. Wild-type Col-0 and the dorn1-3 A. thaliana knockout mutant were used. Examination of the leaf epidermis dorn1-3 mutant provided evidence that P2K1/DORN1 is a part of the signal transduction pathway in stomatal closure evoked by extracellular Ap4A but not by Cp4C. Reactive oxygen species (ROS) are involved in signal transduction caused by Ap4A and Cp4C, leading to stomatal closure. Ap4A induced and Cp4C suppressed the transcriptional response in wild-type plants. Moreover, in dorn1-3 leaves, the effect of Ap4A on gene expression was impaired. The interaction between P2K1/DORN1 and Ap4A leads to changes in the transcription of signalling hubs in signal transduction pathways.

وصف الملف: application/pdf

العلاقة: Dobrogojski, J.; Nguyen, V.H.; Kowalska, J.; Borek, S.; Pietrowska-Borek, M. The Plasma Membrane Purinoreceptor P2K1/DORN1 Is Essential in Stomatal Closure Evoked by Extracellular Diadenosine Tetraphosphate (Ap4A) in Arabidopsis thaliana. Int. J. Mol. Sci. 2023, 24, 16688. https://doi.org/10.3390Test/ ijms242316688; https://depot.ceon.pl/handle/123456789/23287Test

الإتاحة: https://doi.org/10.3390/ijms242115974Test
https://depot.ceon.pl/handle/123456789/23287Test

المؤلفون: Ted C. J. Turlings, Thomas Degen

المصدر: CHIMIA, Vol 76, Iss 11 (2022)

مصطلحات موضوعية: plant volatiles, plant defence, plant signalling, plant-insect interactions, Chemistry, QD1-999

الوصف: It is increasingly evident that plants actively respond to the threats and challenges that they come to face while growing. This is particularly manifested in the dynamic responses to insect herbivory, especially in terms of the volatile compounds that the attacked plants emit. Indeed, many plants respond to insect-inflicted damage with the synthesis and release of volatile organic compounds. These emissions, commonly referred to as herbivore-induced plant volatiles (HIPVs), play important roles in the interactions between the emitting plants and their biotic environment. The odorous signal can be picked up and exploited by various organisms: neighbouring plants, herbivores and their natural enemies, such as predators and parasitoid wasps. Coincidence or not, scientists currently working in Switzerland have made numerous key contributions to the work in this field. By highlighting their work, we attempt to give a somewhat historic overview of this field of research.

وصف الملف: electronic resource

العلاقة: https://www.chimia.ch/chimia/article/view/6171Test; https://doaj.org/toc/0009-4293Test; https://doaj.org/toc/2673-2424Test

الوصول الحر: https://doaj.org/article/8236b6ca15eb454a9b6f9481b5bf9d3cTest