المؤلفون: Huimei Wang, Shen Ni, Guan Li, Zhiyong Li, Shaoqing Tang, Liqun Tang, Xixi Liu, Yazhou Shu, Jian Zhang, Wenfei Xiao, Huayu Xu, Yifeng Wang, Jie-Zheng Ying, Wanning Liu, Xiaohong Tong, Jialing Yao

المصدر: International Journal of Molecular Sciences
Volume 22
Issue 22
International Journal of Molecular Sciences, Vol 22, Iss 12220, p 12220 (2021)

مصطلحات موضوعية: QH301-705.5, gibberellins, Mutant, semi-dwarf, Regulator, rice (Oryza sativa L.), seed germination, Germination, Biology, Article, Catalysis, Inorganic Chemistry, Gene Expression Regulation, Plant, Transcription (biology), bZIP, Gene silencing, Physical and Theoretical Chemistry, Biology (General), Molecular Biology, Psychological repression, QD1-999, Spectroscopy, Plant Proteins, Oryza sativa, Organic Chemistry, food and beverages, Oryza, General Medicine, Computer Science Applications, Cell biology, Chemistry, Basic-Leucine Zipper Transcription Factors, polycomb repression complex, Seeds, Gibberellin

الوصف: Gibberellins (GAs) are diterpenoid phytohormones regulating various aspects of plant growth and development, such as internode elongation and seed germination. Although the GA biosynthesis pathways have been identified, the transcriptional regulatory network of GA homeostasis still remains elusive. Here, we report the functional characterization of a GA-inducible OsABF1 in GA biosynthesis underpinning plant height and seed germination. Overexpression of OsABF1 produced a typical GA-deficient phenotype with semi-dwarf and retarded seed germination. Meanwhile, the phenotypes could be rescued by exogenous GA3, suggesting that OsABF1 is a key regulator of GA homeostasis. OsABF1 could directly suppress the transcription of green revolution gene SD1, thus reducing the endogenous GA level in rice. Moreover, OsABF1 interacts with and transcriptionally antagonizes to the polycomb repression complex component OsEMF2b, whose mutant showed as similar but more severe phenotype to OsABF1 overexpression lines. It is suggested that OsABF1 recruits RRC2-mediated H3K27me3 deposition on the SD1 promoter, thus epigenetically silencing SD1 to maintain the GA homeostasis for growth and seed germination. These findings shed new insight into the functions of OsABF1 and regulatory mechanism underlying GA homeostasis in rice.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2a19a700cfa106a0f1e88ecc8f75228Test

المؤلفون: Wanning Liu, Liqun Tang, Xixi Liu, Shuang Wang, Xiaohong Tong, Jian Zhang, Shufan Li, Juan Zhao, Abolore Adijat Ajadi, Yifeng Wang, Yazhou Shu, Zhiyong Li, Huimei Wang, Sani Muhammad Tajo

المصدر: International Journal of Molecular Sciences, Vol 21, Iss 1, p 245 (2019)
International Journal of Molecular Sciences
Volume 21
Issue 1

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, grain filling, Cell Cycle Proteins, 01 natural sciences, lcsh:Chemistry, chemistry.chemical_compound, cyclin-dependent kinase inhibitors, Gene Expression Regulation, Plant, Brassinosteroid, Abscisic acid, lcsh:QH301-705.5, Spectroscopy, Cyclin-Dependent Kinase Inhibitor Proteins, Plant Proteins, biology, Protoplasts, Gene Expression Regulation, Developmental, food and beverages, General Medicine, Cell cycle, Cyclin-Dependent Kinases, Computer Science Applications, Cell biology, Germination, Seeds, seed germination, rice (oryza sativa l.), Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, Brassinosteroids, Endoreduplication, Physical and Theoretical Chemistry, Molecular Biology, Cell Proliferation, Cell Nucleus, Oryza sativa, Arabidopsis Proteins, Cell growth, Organic Chemistry, Oryza, biology.organism_classification, 030104 developmental biology, chemistry, lcsh:Biology (General), lcsh:QD1-999, Seedling, Mutation, rice (Oryza sativa L.), Edible Grain, Abscisic Acid, 010606 plant biology & botany

الوصف: Cyclin-dependent kinase inhibitors known as KRPs (kip-related proteins) control the progression of plant cell cycles and modulate various plant developmental processes. However, the function of KRPs in rice remains largely unknown. In this study, two rice KRPs members, KRP1 and KRP2, were found to be predominantly expressed in developing seeds and were significantly induced by exogenous abscisic acid (ABA) and Brassinosteroid (BR) applications. Sub-cellular localization experiments showed that KRP1 was mainly localized in the nucleus of rice protoplasts. KRP1 overexpression transgenic lines (OxKRP1), krp2 single mutant (crkrp2), and krp1/krp2 double mutant (crkrp1/krp2) all exhibited significantly smaller seed width, seed length, and reduced grain weight, with impaired seed germination and retarded early seedling growth, suggesting that disturbing the normal steady state of KRP1 or KRP2 blocks seed development partly through inhibiting cell proliferation and enlargement during grain filling and seed germination. Furthermore, two cyclin-dependent protein kinases, CDKC
2 and CDKF
3, could interact with KRP1 in a yeast-two-hybrid system, indicating that KRP1 might regulate the mitosis cell cycle and endoreduplication through the two targets. In a word, this study shed novel insights into the regulatory roles of KRPs in rice seed maturation and germination.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0659e0f1c5f0182a0835e8d27201ac20Test
https://www.mdpi.com/1422-0067/21/1/245Test