دورية أكاديمية
DNA remodelling by Strict Partial Endoreplication in orchids, an original process in the plant kingdom
| العنوان: | DNA remodelling by Strict Partial Endoreplication in orchids, an original process in the plant kingdom |
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| المؤلفون: | Brown, Spencer C., Bourge, Mickaël, Maunoury, Nicolas, Wong, Maurice, Bianchi, Michele Wolfe, Lepers-Andrzejewski, Sandra, Besse, Pascale, Siljak-Yakovlev, Sonja, Dron, Michel, Satiat-Jeunemaître, Béatrice |
| المساهمون: | Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre National de la Recherche Scientifique (CNRS), Université Paris Sud (Paris 11), Université Paris-Saclay, Plateforme intégrée IMAGIF, Unite de Formation et de Recherche Sciences et Technologie, Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université de La Réunion (UR), Laboratory for Research and Protection of Endemic Resources, University of Sarajevo, Virologie et Immunologie Moléculaires (VIM (UR 0892)), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Biologie Cellulaire (BioCell), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Dynamique de la Compartimentation cellulaire dans les cellules de plantes supérieures (DYNBSJ), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Cytométrie (CYTO), Département Plateforme (PF I2BC), Programme d'Investissement d'Avenir "France-BioImaging" (Agence Nationale de la Recherche) ANR-10-ISBN-04-01, Labex "Saclay Plant Science" (Agence Nationale de la recherche) ANR-11-IDEX-0003-02, French Agence Nationale de la Recherche through the Endorepigen grant ANR-09-GENM-105, Vabiome (ERANET Netbiome) 11-EBIM-005-01, 11-EBIM-005-05, 11-EBIM-005-04, 11-EBIM-005-06, ANR-11-IDEX-0003,IPS,Idex Paris-Saclay(2011) |
| المصدر: | ISSN: 1759-6653. |
| بيانات النشر: | HAL CCSD Society for Molecular Biology and Evolution |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Genome Size, Vanilla, PF, BIOCELL, CYTO, DYNBSJ, MINION, IMAGIF, PHOT, cytogenetics, cytometry, endoreplication, genome imbalance, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; DNA remodelling during endoreplication appears to be a strong developmental characteristic in orchids. In this study, we analysed DNA content and nuclei in 41 species of orchids to further map the genome evolution in this plant family. We demonstrate that the DNA remodelling observed in 36 out of 41 orchids studied corresponds to strict partial endoreplication. Such process is developmentally regulated in each wild species studied. Cytometry data analyses allowed us to propose a model where nuclear states 2C, 4E, 8E, etc. form a series comprising a fixed proportion, the euploid genome 2C, plus 2 to 32 additional copies of a complementary part of the genome. The fixed proportion ranged from 89% of the genome in Vanilla mexicana down to 19% in V. pompona, the lowest value for all 148 orchids reported. Insterspecific hybridisation did not suppress this phenomenon. Interestingly, this process was not observed in mass-produced epiphytes. Nucleolar volumes grow with the number of endocopies present, coherent with high transcription activity in endoreplicated nuclei. Our analyses suggest species-specific chromatin rearrangement. Towards understanding endoreplication, V. planifolia constitutes a tractable system for isolating the genomic sequences that confer an advantage via endoreplication from those that apparently suffice at diploid level. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/28419219; hal-02187753; https://hal.science/hal-02187753Test; https://hal.science/hal-02187753/documentTest; https://hal.science/hal-02187753/file/2017_Brown_GBE_1.pdfTest; PRODINRA: 408708; PUBMED: 28419219; WOS: 000406755800021 |
| DOI: | 10.1093/gbe/evx063 |
| الإتاحة: | https://doi.org/10.1093/gbe/evx063Test https://hal.science/hal-02187753Test https://hal.science/hal-02187753/documentTest https://hal.science/hal-02187753/file/2017_Brown_GBE_1.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.F89D9213 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/gbe/evx063 |
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