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1دورية أكاديمية
المؤلفون: Alerie Guzman De La Fuente, Simona Lange, Maria Elena Silva, Ginez A. Gonzalez, Herbert Tempfer, Peter van Wijngaarden, Chao Zhao, Ludovica Di Canio, Andrea Trost, Lara Bieler, Pia Zaunmair, Peter Rotheneichner, Anna O’Sullivan, Sebastien Couillard-Despres, Oihana Errea, Maarja A. Mäe, Johanna Andrae, Liqun He, Annika Keller, Luis F. Bátiz, Christer Betsholtz, Ludwig Aigner, Robin J.M. Franklin, Francisco J. Rivera
المصدر: Cell Reports, Vol 20, Iss 8, Pp 1755-1764 (2017)
مصطلحات موضوعية: neurovascular niche, pericytes, remyelination, oligodendrocyte progenitor cell, Lama2, Biology (General), QH301-705.5
الوصف: The role of the neurovascular niche in CNS myelin regeneration is incompletely understood. Here, we show that, upon demyelination, CNS-resident pericytes (PCs) proliferate, and parenchymal non-vessel-associated PC-like cells (PLCs) rapidly develop. During remyelination, mature oligodendrocytes were found in close proximity to PCs. In Pdgfbret/ret mice, which have reduced PC numbers, oligodendrocyte progenitor cell (OPC) differentiation was delayed, although remyelination proceeded to completion. PC-conditioned medium accelerated and enhanced OPC differentiation in vitro and increased the rate of remyelination in an ex vivo cerebellar slice model of demyelination. We identified Lama2 as a PC-derived factor that promotes OPC differentiation. Thus, the functional role of PCs is not restricted to vascular homeostasis but includes the modulation of adult CNS progenitor cells involved in regeneration.
وصف الملف: electronic resource
العلاقة: http://www.sciencedirect.com/science/article/pii/S2211124717310951Test; https://doaj.org/toc/2211-1247Test
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2دورية أكاديمية
المؤلفون: Maria Elena Silva, Simona Lange, Bryan Hinrichsen, Amber R. Philp, Carolina R. Reyes, Diego Halabi, Josselyne B. Mansilla, Peter Rotheneichner, Alerie Guzman de la Fuente, Sebastien Couillard-Despres, Luis F. Bátiz, Robin J. M. Franklin, Ludwig Aigner, Francisco J. Rivera
المصدر: Frontiers in Cellular Neuroscience, Vol 13 (2019)
مصطلحات موضوعية: pericytes, neural stem cells, oligodendrogenesis, Lama2, remyelination, vascular niche, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
الوصف: Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS). Upon demyelination, oligodendrocyte progenitor cells (OPCs) are activated and they proliferate, migrate and differentiate into myelin-producing oligodendrocytes. Besides OPCs, neural stem cells (NSCs) may respond to demyelination and generate oligodendrocytes. We have recently shown that CNS-resident pericytes (PCs) respond to demyelination, proliferate and secrete Laminin alpha2 (Lama2) that, in turn, enhances OPC differentiation. Here, we aimed to evaluate whether PCs influence the fate choice of NSCs in vitro, towards the production of new myelin-producing cells. Indeed, upon exposure to conditioned medium derived from PCs (PC-CM), the majority of NSCs gave rise to GalC- and myelin basic protein (MBP)-expressing oligodendrocytes at the expense of the generation of GFAP-positive astrocytes. Consistent with these findings, PC-CM induces an increase in the expression of the oligodendrocyte fate determinant Olig2, while the expression level of the astrocyte determinant ID2 is decreased. Finally, pre-incubation of PC-CM with an anti-Lama2 antibody prevented the generation of oligodendrocytes. Our findings indicate that PCs-derived Lama2 instructs NSCs to an oligodendrocyte fate choice favoring the generation of myelin-producing cells at the expense of astrocytes in vitro. Further studies aiming to reveal the role of PCs during remyelination may pave the way for the development of new therapies for the treatment of MS.
وصف الملف: electronic resource
العلاقة: https://www.frontiersin.org/article/10.3389/fncel.2019.00085/fullTest; https://doaj.org/toc/1662-5102Test
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3دورية أكاديمية
المؤلفون: Silva, Maria Elena, Lange, Simona, Hinrichsen, Bryan, Philp, Amber R, Reyes, Carolina R, Halabi, Diego, Mansilla, Josselyne B, Rotheneichner, Peter, Guzman de la Fuente, Alerie, Couillard-Despres, Sebastien, Bátiz, Luis F, Franklin, Robin JM, Aigner, Ludwig, Rivera, Francisco J
مصطلحات موضوعية: Lama2, neural stem cells, oligodendrogenesis, pericytes, remyelination, vascular niche
الوصف: Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS). Upon demyelination, oligodendrocyte progenitor cells (OPCs) are activated and they proliferate, migrate and differentiate into myelin-producing oligodendrocytes. Besides OPCs, neural stem cells (NSCs) may respond to demyelination and generate oligodendrocytes. We have recently shown that CNS-resident pericytes (PCs) respond to demyelination, proliferate and secrete Laminin alpha2 (Lama2) that, in turn, enhances OPC differentiation. Here, we aimed to evaluate whether PCs influence the fate choice of NSCs in vitro, towards the production of new myelin-producing cells. Indeed, upon exposure to conditioned medium derived from PCs (PC-CM), the majority of NSCs gave rise to GalC- and myelin basic protein (MBP)-expressing oligodendrocytes at the expense of the generation of GFAP-positive astrocytes. Consistent with these findings, PC-CM induces an increase in the expression of the oligodendrocyte fate determinant Olig2, while the expression level of the astrocyte determinant ID2 is decreased. Finally, pre-incubation of PC-CM with an anti-Lama2 antibody prevented the generation of oligodendrocytes. Our findings indicate that PCs-derived Lama2 instructs NSCs to an oligodendrocyte fate choice favoring the generation of myelin-producing cells at the expense of astrocytes in vitro. Further studies aiming to reveal the role of PCs during remyelination may pave the way for the development of new therapies for the treatment of MS. ; The authors would like to thank the following funding agencies for their support: Paracelsus Medical University PMU-FFF Long-Term Fellowship L-12/01/001-RIV (to and Stand-Alone Grant E-12/15/077-RIT (both to F.J.R.); Chilean FONDECYT Program CONICYT Grant Nº 1161787 (F.J.R.), Grant Nº 1141015 (L.F.B); Chilean PCI Program CONICYT Grant Nº REDES170233, Grant Nº REDES180139 and Grant Nº REDI170037 (all to F.J.R); Chilean FONDEF-IDeA Program Grant Nº ID17AM0043 (M.E.S. and F.J.R.); the Bavarian State ...
وصف الملف: Electronic-eCollection; application/pdf
الإتاحة: https://doi.org/10.17863/CAM.38187Test
https://www.repository.cam.ac.uk/handle/1810/291008Test -
4دورية أكاديمية
المؤلفون: De la Fuente, Alerie Guzman, Lange, Simona, Silva, Maria E, Gonzalez, Ginez A, Tempfer, Herbert, van Wijngaarden, Peter, Zhao, C, Di Canio, Ludovica, Trost, Andrea, Bieler, Lara, Zaunmair, Pia, Rotheneicher, Peter, O'Sullivan, Anna, Couillard-Despres, Sebastien, Errea, Oihana, Mäe, Maarja A, Andrae, Johanna, He, Liqun, Keller, Annika, Bátiz, Luis F, Betsholtz, Christer, Aigner, Ludwig, Franklin, Robin JM, Rivera, Francisco J
المصدر: Cell Reports 20(8) 1755-1764
مصطلحات موضوعية: Lama2, neurovascular niche, oligodendrocyte progenitor cell, pericytes, remyelination
الوصف: The role of the neurovascular niche in CNS myelin regeneration is incompletely understood. Here, we show that, upon demyelination, CNS-resident pericytes (PCs) proliferate, and parenchymal non-vessel-associated PC-like cells (PLCs) rapidly develop. During remyelination, mature oligodendrocytes were found in close proximity to PCs. In Pdgfbret/ret mice, which have reduced PC numbers, oligodendrocyte progenitor cell (OPC) differentiation was delayed, although remyelination proceeded to completion. PC-conditioned medium accelerated and enhanced OPC differentiation in vitro and increased the rate of remyelination in an ex vivo cerebellar slice model of demyelination. We identified Lama2 as a PC-derived factor that promotes OPC differentiation. Thus, the functional role of PCs is not restricted to vascular homeostasis but includes the modulation of adult CNS progenitor cells involved in regeneration.
العلاقة: info:eu-repo/grantAgreement/EC/FP7/278850/; https://zenodo.org/communities/inmindTest; https://zenodo.org/record/931861Test; https://doi.org/10.1016/j.celrep.2017.08.007Test; oai:zenodo.org:931861
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5دورية أكاديمية
المؤلفون: Franklin, RJM
مصطلحات موضوعية: neurovascular niche, pericytes, remyelination, oligodendrocyte progenitor cell, Lama2
الوصف: The role of the neurovascular niche in CNS myelin regeneration is incompletely understood. Here, we show that, upon demyelination, CNS-resident pericytes (PCs) proliferate, and parenchymal non-vessel-associated PC-like cells (PLCs) rapidly develop. During remyelination, mature oligodendrocytes were found in close proximity to PCs. In Pdgfbret/ret mice, which have reduced PC numbers, oligodendrocyte progenitor cell (OPC) differentiation was delayed, although remyelination proceeded to completion. PC-conditioned medium accelerated and enhanced OPC differentiation in vitro and increased the rate of remyelination in an ex vivo cerebellar slice model of demyelination. We identified Lama2 as a PC-derived factor that promotes OPC differentiation. Thus, the functional role of PCs is not restricted to vascular homeostasis but includes the modulation of adult CNS progenitor cells involved in regeneration. ; This work was supported by research funds from Chilean FONDECYT Program CONICYT Grants 1161787 (to F.J.R.) and 1141015 (to L.F.B); Direccion de Investigación y Desarrollo-Universidad Austral de Chile (DID-UACh); Paracelsus Medical University PMU-FFF Long-Term Fellowship L-12/01/001-RIV (to F.J.R.) and Stand-Alone Grant E-12/15/077-RIT (to F.J.R. and A.T.); the European Union Seventh Framework Program (FP7/2007-2013) under Grant Agreements HEALTH-F2-2011-278850 (INMiND), HEALTH-F2-2011-279288 (IDEA), and FP7-REGPOT-316120 (GlowBrain); Austrian Science Fund FWF Special Research Program (SFB) F44 (F4413-B23) “Cell Signaling in Chronic CNS Disorders”; and State Government of Salzburg, Austria (Stiftungsprofessur and 20204-WISS/80/199-2014). In addition, this study was supported by grants from a core support grant from the Wellcome Trust (203151/Z/16/Z) and MRC to the Wellcome Trust–Medical Research Council Cambridge Stem Cell Institute, the UK Multiple Sclerosis Society (941/11), the David and Isobel Walker Trust, “Investissements d’avenir” IHU-A-ICM and Obra Social La Caixa, the Swedish Science Council (2015-00550), the ...
وصف الملف: application/pdf
الإتاحة: https://doi.org/10.17863/CAM.12297Test
https://www.repository.cam.ac.uk/handle/1810/267485Test -
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المؤلفون: Maria Elena Silva, Simona Lange, Bryan Hinrichsen, Amber R. Philp, Carolina R. Reyes, Diego Halabi, Josselyne B. Mansilla, Peter Rotheneichner, Alerie Guzman de la Fuente, Sebastien Couillard-Despres, Luis F. Bátiz, Robin J. M. Franklin, Ludwig Aigner, Francisco J. Rivera
المساهمون: Franklin, Robin [0000-0001-6522-2104], Apollo - University of Cambridge Repository
المصدر: Frontiers in Cellular Neuroscience
Frontiers in Cellular Neuroscience, Vol 13 (2019)مصطلحات موضوعية: 0301 basic medicine, vascular niche, Library science, Lama2, Brief Research Report, pericytes, lcsh:RC321-571, 3. Good health, 03 medical and health sciences, Cellular and Molecular Neuroscience, 030104 developmental biology, 0302 clinical medicine, remyelination, nervous system, oligodendrogenesis, Political science, media_common.cataloged_instance, Vascular niche, Christian ministry, European union, lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry, 030217 neurology & neurosurgery, media_common, Neuroscience, neural stem cells
الوصف: Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS). Upon demyelination, oligodendrocyte progenitor cells (OPCs) are activated and they proliferate, migrate and differentiate into myelin-producing oligodendrocytes. Besides OPCs, neural stem cells (NSCs) may respond to demyelination and generate oligodendrocytes. We have recently shown that CNS-resident pericytes (PCs) respond to demyelination, proliferate and secrete Laminin alpha2 (Lama2) that, in turn, enhances OPC differentiation. Here, we aimed to evaluate whether PCs influence the fate choice of NSCs in vitro, towards the production of new myelin-producing cells. Indeed, upon exposure to conditioned medium derived from PCs (PC-CM), the majority of NSCs gave rise to GalC- and myelin basic protein (MBP)-expressing oligodendrocytes at the expense of the generation of GFAP-positive astrocytes. Consistent with these findings, PC-CM induces an increase in the expression of the oligodendrocyte fate determinant Olig2, while the expression level of the astrocyte determinant ID2 is decreased. Finally, pre-incubation of PC-CM with an anti-Lama2 antibody prevented the generation of oligodendrocytes. Our findings indicate that PCs-derived Lama2 instructs NSCs to an oligodendrocyte fate choice favoring the generation of myelin-producing cells at the expense of astrocytes in vitro. Further studies aiming to reveal the role of PCs during remyelination may pave the way for the development of new therapies for the treatment of MS.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6688205040fcd2b8162c8330b9b3ea9aTest
https://pubmed.ncbi.nlm.nih.gov/30971893Test -
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المؤلفون: Maria Elena Silva, Simona Lange, Bryan Hinrichsen, Amber R. Philp, Carolina R. Reyes, Diego Halabi, Josselyne B. Mansilla, Peter Rotheneichner, Alerie Guzman de la Fuente, Sebastien Couillard-Despres, Luis F. Bátiz, Robin J. M. Franklin, Ludwig Aigner, Francisco J. Rivera
مصطلحات موضوعية: Cell Biology, Neuroscience, Cellular Nervous System, Central Nervous System, Cellular Interactions (incl. Adhesion, Matrix, Cell Wall), Protein Trafficking, pericytes, neural stem cells, oligodendrogenesis, Lama2, remyelination, vascular niche
الوصف: Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS). Upon demyelination, oligodendrocyte progenitor cells (OPCs) are activated and they proliferate, migrate and differentiate into myelin-producing oligodendrocytes. Besides OPCs, neural stem cells (NSCs) may respond to demyelination and generate oligodendrocytes. We have recently shown that CNS-resident pericytes (PCs) respond to demyelination, proliferate and secrete Laminin alpha2 (Lama2) that, in turn, enhances OPC differentiation. Here, we aimed to evaluate whether PCs influence the fate choice of NSCs in vitro, towards the production of new myelin-producing cells. Indeed, upon exposure to conditioned medium derived from PCs (PC-CM), the majority of NSCs gave rise to GalC- and myelin basic protein (MBP)-expressing oligodendrocytes at the expense of the generation of GFAP-positive astrocytes. Consistent with these findings, PC-CM induces an increase in the expression of the oligodendrocyte fate determinant Olig2, while the expression level of the astrocyte determinant ID2 is decreased. Finally, pre-incubation of PC-CM with an anti-Lama2 antibody prevented the generation of oligodendrocytes. Our findings indicate that PCs-derived Lama2 instructs NSCs to an oligodendrocyte fate choice favoring the generation of myelin-producing cells at the expense of astrocytes in vitro. Further studies aiming to reveal the role of PCs during remyelination may pave the way for the development of new therapies for the treatment of MS.
الإتاحة: https://doi.org/10.3389/fncel.2019.00085.s001Test
https://figshare.com/articles/dataset/Table_1_Pericytes_Favor_Oligodendrocyte_Fate_Choice_in_Adult_Neural_Stem_Cells_DOCX/7902326Test -
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المؤلفون: Silva, Maria Elena, Lange, Simona, Hinrichsen, Bryan, Philp, Amber R, Reyes, Carolina R, Halabi, Diego, Mansilla, Josselyne B, Rotheneichner, Peter, Guzman De La Fuente, Alerie, Couillard-Despres, Sebastien, Bátiz, Luis F, Franklin, Robin JM, Aigner, Ludwig, Rivera, Francisco J
مصطلحات موضوعية: remyelination, vascular niche, nervous system, oligodendrogenesis, Lama2, pericytes, 3. Good health, neural stem cells
الوصف: Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS). Upon demyelination, oligodendrocyte progenitor cells (OPCs) are activated and they proliferate, migrate and differentiate into myelin-producing oligodendrocytes. Besides OPCs, neural stem cells (NSCs) may respond to demyelination and generate oligodendrocytes. We have recently shown that CNS-resident pericytes (PCs) respond to demyelination, proliferate and secrete Laminin alpha2 (Lama2) that, in turn, enhances OPC differentiation. Here, we aimed to evaluate whether PCs influence the fate choice of NSCs in vitro, towards the production of new myelin-producing cells. Indeed, upon exposure to conditioned medium derived from PCs (PC-CM), the majority of NSCs gave rise to GalC- and myelin basic protein (MBP)-expressing oligodendrocytes at the expense of the generation of GFAP-positive astrocytes. Consistent with these findings, PC-CM induces an increase in the expression of the oligodendrocyte fate determinant Olig2, while the expression level of the astrocyte determinant ID2 is decreased. Finally, pre-incubation of PC-CM with an anti-Lama2 antibody prevented the generation of oligodendrocytes. Our findings indicate that PCs-derived Lama2 instructs NSCs to an oligodendrocyte fate choice favoring the generation of myelin-producing cells at the expense of astrocytes in vitro. Further studies aiming to reveal the role of PCs during remyelination may pave the way for the development of new therapies for the treatment of MS.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::c5c1c680117b34a30cc27296bde7ff08Test
النص الكامل