دورية أكاديمية
Antigenic characterization of the human immunodeficiency virus (HIV-1) envelope glycoprotein precursor incorporated into nanodiscs
| العنوان: | Antigenic characterization of the human immunodeficiency virus (HIV-1) envelope glycoprotein precursor incorporated into nanodiscs |
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| المؤلفون: | Witt, Kristen C., Castillo-Menendez, Luis, Ding, Haitao, Espy, Nicole, Zhang, Shijian, Kappes, John C., Sodroski, Joseph |
| بيانات النشر: | Public Library of Science |
| سنة النشر: | 2017 |
| المجموعة: | Harvard University: DASH - Digital Access to Scholarship at Harvard |
| مصطلحات موضوعية: | Immunologic Techniques, Immunoassays, Enzyme-Linked Immunoassays, Biology and Life Sciences, Microbiology, Medical Microbiology, Microbial Pathogens, Viral Pathogens, Immunodeficiency Viruses, HIV, HIV-1, Medicine and Health Sciences, Pathology and Laboratory Medicine, Pathogens, Organisms, Viruses, RNA viruses, Retroviruses, Lentivirus, Biochemistry, Glycobiology, Glycoproteins, Physical Sciences, Materials Science, Materials by Attribute, Surfactants, Detergents, Physiology, Immune Physiology, Antibodies |
| الوصف: | The entry of human immunodeficiency virus (HIV-1) into host cells is mediated by the viral envelope glycoproteins (Envs), which are derived by the proteolytic cleavage of a trimeric gp160 Env precursor. The mature Env trimer is a major target for entry inhibitors and vaccine-induced neutralizing antibodies. Env interstrain variability, conformational flexibility and heavy glycosylation contribute to evasion of the host immune response, and create challenges for structural characterization and vaccine development. Here we investigate variables associated with reconstitution of the HIV-1 Env precursor into nanodiscs, nanoscale lipid bilayer discs enclosed by membrane scaffolding proteins. We identified detergents, as well as lipids similar in composition to the viral lipidome, that allowed efficient formation of Env-nanodiscs (Env-NDs). Env-NDs were created with the full-length Env precursor and with an Env precursor with the majority of the cytoplasmic tail intact. The self-association of Env-NDs was decreased by glutaraldehyde crosslinking. The Env-NDs exhibited an antigenic profile expected for the HIV-1 Env precursor. Env-NDs were recognized by broadly neutralizing antibodies. Of note, neutralizing antibody epitopes in the gp41 membrane-proximal external region and in the gp120:gp41 interface were well exposed on Env-NDs compared with Env expressed on cell surfaces. Most Env epitopes recognized by non-neutralizing antibodies were masked on the Env-NDs. This antigenic profile was stable for several days, exhibiting a considerably longer half-life than that of Env solubilized in detergents. Negative selection with weak neutralizing antibodies could be used to improve the antigenic profile of the Env-NDs. Finally, we show that lipid adjuvants can be incorporated into Env-NDs. These results indicate that Env-NDs represent a potentially useful platform for investigating the structural, functional and antigenic properties of the HIV-1 Env trimer in a membrane context. ; Version of Record |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289478/pdfTest/; PLoS ONE; Witt, Kristen C., Luis Castillo-Menendez, Haitao Ding, Nicole Espy, Shijian Zhang, John C. Kappes, and Joseph Sodroski. 2017. “Antigenic characterization of the human immunodeficiency virus (HIV-1) envelope glycoprotein precursor incorporated into nanodiscs.” PLoS ONE 12 (2): e0170672. doi:10.1371/journal.pone.0170672. http://dx.doi.org/10.1371/journal.pone.0170672Test.; http://nrs.harvard.edu/urn-3:HUL.InstRepos:31731890Test |
| DOI: | 10.1371/journal.pone.0170672 |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0170672Test http://nrs.harvard.edu/urn-3:HUL.InstRepos:31731890Test |
| رقم الانضمام: | edsbas.823BB5C2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0170672 |
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