المؤلفون: Hansol Park, Yeseul Kang, Mohammed Mebarek Bia, Keeseon S. Eom, Tilak Chandra Nath, Seongjun Choe, Sunmin Kim, Saiful Islam, Anwar Hossain, Siblee Sadik Sabuj, Barakaeli Abdieli Ndosi, Dongmin Lee

المصدر: International Journal of Infectious Diseases, Vol 110, Iss, Pp 449-456 (2021)

مصطلحات موضوعية: Microbiology (medical), Veterinary medicine, Trichuris, Capillaria, Infectious and parasitic diseases, RC109-216, geohelminths, Soil, Zoonoses, parasitic diseases, Animals, Humans, Helminths, Ascaridia galli, Bangladesh, biology, Ascaris, business.industry, diagnostic methods, General Medicine, Contamination, biology.organism_classification, Cross-Sectional Studies, Infectious Diseases, Heterakis gallinarum, Feasibility Studies, Livestock, business, feasibility

الوصف: Introduction The objectives of this study were to evaluate a modified method for isolating geohelminth eggs and to assess the geohelminth contamination in Bangladesh. Methods The efficacy of the modified method was evaluated using naturally contaminated and experimentally seeded soil samples. In total, 240 samples were assessed from four different sites in three geographic contexts. A questionnaire survey with 50 professionals was conducted. Results The modified method showed considerable efficacy in isolating parasitic eggs from naturally contaminated soil (54.0%) and experimentally seeded soils (63.0% for Toxocara eggs and 52.0% for Ascaris eggs). The modified method was described as convenient by the majority of participants. Overall prevalence was 52.5%, with several species of helminth observed, including Toxocara sp., Ascaridia galli/Heterakis gallinarum, Ascaris sp., hookworms/strongyles, Capillaria sp., Trichuris sp., and taeniids). The contamination rate was found to be higher around livestock farms (76.7%), followed by latrines (63.3%), households (41.6%), and schools (28.3%). Conclusions The modified method was shown to be feasible in terms of field applicability and egg recovery rate, and could be adopted in low-resource settings. A substantial prevalence of geohelminths was observed, with some of the species associated with zoonoses. These findings highlight the urgent need for widespread mapping of geohelminths to avoid spillovers to animals and humans.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12612f6b37d2903172014c59e94f456eTest
https://doi.org/10.1016/j.ijid.2021.08.006Test

المؤلفون: Taehee Chang, Hyejoo Shin, Seungwan Ryoo, Hansol Park, Keeseon S. Eom, Woon-Mok Sohn, Keon Hoon Lee, Virak Khieu, Rekol Huy, Jeonggyu Lee, Sooji Hong, Bong-Kwang Jung, Jong-Yil Chai

المصدر: The Korean Journal of Parasitology

مصطلحات موضوعية: Adult, Male, Mitochondrial DNA, Nuclear gene, 030231 tropical medicine, Locus (genetics), macromolecular substances, Haploidy, Brief Communication, 030308 mycology & parasitology, Microbiology, Electron Transport Complex IV, 03 medical and health sciences, 0302 clinical medicine, Genotype, parasitic diseases, allelic study, medicine, Animals, Humans, Allele, Phylogeny, Taeniasis, Taenia asiatica, 0303 health sciences, biology, Taenia, hybrid, Chimera, Taenia saginata, Helminth Proteins, biology.organism_classification, Praziquantel, Infectious Diseases, Parasitology, Cambodia, medicine.drug

الوصف: Human infection with Taenia asiatica or a hybrid between Taenia saginata and T. asiatica has not been reported in Cambodia. We detected for the first time a hybrid form between T. saginata and T. asiatica in Preah Vihear Province, Cambodia. An adult tapeworm specimen, i.e., 75 cm long strobila without scolex, was expelled from a 27-year-old man after praziquantel medication and purging. It was morphologically indistinguishable between T. saginata and T. asiatica. Several proglottids were molecularly analyzed to confirm the tapeworm species. The mitochondrial gene encoding cytochrome c oxidase subunit 1 (cox1) and nuclear genes encoding elongation factor-1α (ef1) and ezrin-radixin-moesin (ERM)-like protein (elp) were sequenced, and a single-allele analysis was performed to confirm the haploid genotype. The results revealed that our sample showed a discrepancy between the mitochondrial and 2 nuclear genes. It possessed homozygous sequences typical of T. saginata at cox1 and ef1 loci. However, it was heterozygous at the elp locus, with 1 allele in T. asiatica (elpA) and 1 in T. saginata (elpC), which indicates that it is a hybrid between T. saginata and T. asiatica. The present results confirmed the presence of a hybrid between T. saginata and T. asiatica in Cambodia and strongly suggest the existence of also ‘pure’ T. asiatica in Cambodia.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e14b42b8d8b6e1a49b54c884b884ca9Test
https://pubmed.ncbi.nlm.nih.gov/33951775Test

المؤلفون: Hansol Park, Yeseul Kang, Seongjun Choe, Julius Keyyu, Robert D. Fyumagwa, Dongmin Lee, Hyeong-Kyu Jeon, Sang-Hwa Lee, Mohammed Mebarek Bia, Keeseon S. Eom

المصدر: The Korean Journal of Parasitology

مصطلحات موضوعية: Lions, Male, 0301 basic medicine, Mitochondrial DNA, 030231 tropical medicine, Zoology, Brief Communication, nad1, Tanzania, Host-Parasite Interactions, Feces, 03 medical and health sciences, 0302 clinical medicine, Phylogenetics, biology.animal, parasitic diseases, Animals, Cox1 gene, Spirometra, Phylogeny, lion, Phylogenetic tree, biology, NADH Dehydrogenase, 030108 mycology & parasitology, biology.organism_classification, Spirometra ranarum, cox1, Mitochondria, Infectious Diseases, GenBank, Cyclooxygenase 1, Parasitology, Panthera

الوصف: The present study was performed with morphological and molecular analysis (cox1 and nad1 mitochondrial genes) to identify the proglottids of spirometrid tapeworm found in the stool of an African lion, Panthera leo, in the Serengeti plain of Tanzania. A strand of tapeworm strobila, about 75 cm in length, was obtained in the stool of a male African lion in the Serengeti National Park (34˚ 50' E, 02˚ 30' S), Tanzania, in February 2012. The morphological features of the adult worm examined exhibited 3 uterine coils with a bow tie appearance and adopted a diagonal direction in the second turn. The posterior uterine coils are larger than terminal uterine ball and the feature of uteri are swirling rather than spirally coiling. The sequence difference between the Spirometra species (Tanzania origin) and S. erinaceieuropaei (GenBank no. KJ599680) was 9.4% while those of S. decipiens (GenBank no. KJ599679) differed by 2.1% in the cox1 and nad1 genes. Phylogenetic tree topologies generated using the 2 analytic methods were identical and presented high level of confidence values for the 3 major branches of the 3 Spirometra species in the cox1 gene. The morphological and molecular findings obtained in this study were nearly coincided with those of S. ranarum. Therefore, we can know for the first time that the African lion, Panthera leo, is to the definitive host of this tapeworm.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff97afacb25b35a446acc3d168b38026Test
https://doi.org/10.3347/kjp.2018.56.4.379Test

المؤلفون: Hyeong-Kyu Jeon, Mohammed Mebarek Bia, Yeseul Kang, Sunmin Kim, Chatanun Eamudomkarn, Barakaeli Abdieli Ndosi, Keeseon S. Eom, Tilak Chandra Nath, Seongjun Choe, Hansol Park, Dongmin Lee

المصدر: The Korean Journal of Parasitology

مصطلحات موضوعية: Veterinary medicine, Genotype, Cattle Diseases, Tanzania, sensu stricto, parasitic diseases, medicine, Animals, Echinococcus granulosus, biology, business.industry, Haplotype, biology.organism_classification, medicine.disease, Echinococcus, genetic phylogeny, Infectious Diseases, cattle, Parasitic disease, Parasitology, Livestock, Original Article, business, Echinococcus ortleppi, Echinococcus species

الوصف: Cystic echinococcosis is a zoonotic parasitic disease caused by Echinococcus species. Tanzania is one of the endemic countries with cystic echinococcosis. This study focussed on identifying genotypes of Echinococcus spp. in Tanzania. We collected 7 cysts from cattle in Mwanza municipal (n=4) and Loliondo district (n=3). The cysts from Mwanza were all E. ortleppi and fertile. In contrast, the cysts from Loliondo were all E. granulosus sensu stricto and sterile. Two from the 4 cysts were a new haplotype of E. ortleppi (G5). These results can improve the preventive and control programs for humans and livestock in Tanzania. To our knowledge, this study is considered the first to identify the genotype and haplotype of Echinococcus spp. in Tanzania.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab526b145adcd62e31693b6bc8b7f026Test
https://pubmed.ncbi.nlm.nih.gov/34724764Test

المؤلفون: Hansol Park, Dongmin Lee, Seongjun Choe, Hyeong-Kyu Jeon, Keeseon S. Eom

المصدر: The Korean Journal of Parasitology

مصطلحات موضوعية: 0301 basic medicine, Sequence analysis, 030231 tropical medicine, Cestoda, Zoology, cat, Brief Communication, law.invention, 03 medical and health sciences, Diphyllobothriidae, 0302 clinical medicine, scolex, law, Spirometra decipiens, parasitic diseases, Cox1 gene, Polymerase chain reaction, Direct sequencing, biology, 030108 mycology & parasitology, biology.organism_classification, cox1, molecular detection, Infectious Diseases, Spirometra, Parasitology

الوصف: Morphological and molecular characteristics of spirometrid tapeworms, Spirometra decipiens, were studied, which were recovered from a heavily infected stray cat road-killed in Eumseong-gun, Chungcheongbuk-do (Province), the Republic of Korea (=Korea). A total of 134 scolices and many broken immature and mature proglottids of Spirometra tapeworms were collected from the small intestine of the cat. Morphological observations were based on 116 specimens. The scolex was 22.8-32.6 mm (27.4 mm in average) in length and small spoon-shape with 2 distinct bothria. The uterus was coiled 3-4 times, the end of the uterus was ball-shaped, and the vaginal aperture shaped as a crescent moon was closer to the cirrus aperture than to the uterine aperture. PCR amplification and direct sequencing of the cox1 target fragment (377 bp in length and corresponding to positions 769-1,146 bp of the cox1 gene) were performed using total genomic DNA extracted from 134 specimens. The cox1 sequences (377 bp) of the specimens showed 99.0% similarity to the reference sequence of S. decipiens and 89.3% similarity to the reference sequence of S. erinaceieuropaei. In the present study, we report a stray cat heavily infected with S. decipiens identified by mitochondrial cox1 sequence analysis and morphological examinations of the adult worms.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ae3fd6b8ee0f31f386e1b7ef4ecf37eTest
http://europepmc.org/articles/PMC5858661Test

المؤلفون: Hansol Park, Seongjun Choe, In Yong Lee, Hyeong-Kyu Jeon, Keeseon S. Eom, Dongmin Lee, Ki Jeong Na, Young-Sun Lee

المصدر: The Korean Journal of Parasitology

مصطلحات موضوعية: 0301 basic medicine, Trombiculidae, Trombiculiasis, food.ingredient, red squirrel, Fauna, Cestoda, Zoology, Ectoparasitic Infestations, Catenotaenia dendritica, 03 medical and health sciences, Flea Infestations, food, Republic of Korea, parasitic diseases, Animals, Parasite hosting, Sciurus, Sciurus vulgaris, Microscopy, Korea, Cetatophyllus (Monopsyllus) indages, biology, Leptotrombidium pallidum, Sciuridae, 030108 mycology & parasitology, Cestode Infections, biology.organism_classification, Cheongju, Infectious Diseases, Vector (epidemiology), Ceratophyllus, Siphonaptera, Original Article, Parasitology, Hirstionyssus sciurinus

الوصف: Parasites are recorded from the red squirrel, Sciurus vulgaris, from Cheongju, the Republic of Korea. A total of 5 road-killed squirrels were thoroughly examined for internal and external parasites from November 2011 to May 2014. Total 4 parasite species, including 1 tapeworm and 3 ectoparasite species were recovered. They were morphologically identified as Catenotaenia dendritica (Cestoda: Catenotaeniidae), Hirstionyssus sciurinus, Leptotrombidium pallidum, and Ceratophyllus (Monopsyllus) indages. Among them, C. dendritica and H. sciurinus are recorded for the first time in the Korean parasite fauna. In addition, the possibility that the red squirrel could act as a reservoir host for a zoonotic disease like tsutsugamushi disease with L. pallidum as its vector has been raised.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1112b528863251ff680096a14c09847bTest
https://doi.org/10.3347/kjp.2016.54.4.509Test

المؤلفون: Duk Young Min, Keeseon S. Eom, Woon Mok Sohn, Han Jong Rim, Jong-Yil Chai, Byoung Kuk Na, Tai Soon Yong, Hansol Park

المصدر: The Korean Journal of Parasitology

مصطلحات موضوعية: Animal Experimentation, Biometry, Snails, cat, Digenea, Collar, Echinostomatidae, metacercaria, Lao PDR, Echinostoma, snail, parasitic diseases, Animals, rat, Microscopy, biology, Echinostoma macrorchis, adult, Intermediate host, Animal Structures, Anatomy, Viral tegument, biology.organism_classification, Rats, Infectious Diseases, Laos, Vientiane, Excretory system, Cats, Cipangopaludina, Original Article, Parasitology

الوصف: The echinostome metacercariae encysted in Cipangopaludina sp. snails that were purchased from a market in Vientiane Municipality, Lao PDR, were identified as Echinostoma macrorchis (Digenea: Echinostomatidae) through recovery of adult flukes after experimental infection to rats and a cat. The metacercariae were round, 113-128 (121)×113-125 (120) µm, having a thick cyst wall, a head collar armed with collar spines, and excretory granules. The adult flukes recovered from the rats and cat at day 14 and 30 post-infection, respectively, were elongated, ventrally curved, and 3.9-6.3×0.7-1.1 mm in size. The head collar was distinct, bearing 43-45 collar spines with 5 angle spines on each side. Two testes were large (as the name implies), tandem, and slightly constricted at the middle, with irregular margins. Eggs were operculated, ovoid to elliptical, and 88-95×56-60 µm. In scanning electron microscopy, the head collar was prominent, with 43-45 collar spines. Scale-like tegumental spines were densely distributed on the ventral surface between the oral and ventral suckers. Sensory papillae were distributed mainly on the tegument around the 2 suckers. It is confirmed that E. macrorchis is distributed in Lao PDR using Cipangopaludina sp. snails as the second intermediate host.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8229376901a21ca0352d78e2359b57e8Test
https://doi.org/10.3347/kjp.2013.51.2.191Test

المؤلفون: Seongjun Choe, Dongmin Lee, Hansol Park, Hyeong-Kyu Jeon, Youngsun Lee, Ki-Jeong Na, Shi-Ryong Park, Eom, Keeseon S.

المصدر: Korean Journal of Parasitology; Oct2016, Vol. 54 Issue 5, p659-665, 7p

مصطلحات موضوعية: PARASITIC diseases, ECHINOSTOMATIDAE, ORIENTAL stork, AUTOPSY

مصطلحات جغرافية: KOREA

مستخلص: We intended to describe a case of chaunocephalosis and morphological characteristics of its causative agent, Chaunocephalus ferox, recovered from an oriental white stork, Ciconia boyciana, in the Republic of Korea. An oriental white stork was referred to the Wildlife Center of Chungbuk in Korea in February 2014 for severe depression with cachexia and it died the next day. At necropsy, the stomach was severely expanded and 7 thick-walled nodules were observed in the upper part of the intestine. Although the stomach was filled with full of foreign materials, the intestine was almost empty. The nodules were globular and total 9 flukes were recovered. They were 8,030-8,091 µm in length and 3,318- 3,333 µm in maximum width. Because the flukes had bulbous forebody with short narrow subcylindrical hindbody, 27 collar spines, and vitelline follicles not reaching to the posterior end, the specimens were identified as being C. ferox. The cyst formation induced thickening of the intestinal wall with narrowing of the lumen that could have contributed to the gastric impaction to the death of the host. This is the first described case of chaunocephalosis and its causative agent C. ferox found from an oriental white stork in Korea. [ABSTRACT FROM AUTHOR]

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