المؤلفون: Mansuri M. Tosif, Agnieszka Najda, Aarti Bains, Ravinder Kaushik, Sanju Bala Dhull, Prince Chawla, Magdalena Walasek-Janusz

المصدر: Polymers, Vol 13, Iss 1066, p 1066 (2021)

مصطلحات موضوعية: nanohydrogel, food applications, biopolymers, polysaccharide, Organic chemistry, QD241-441

الوصف: Easily sourced mucus from various plant parts is an odorless, colorless and tasteless substance with emerging commercial potential in agriculture, food, cosmetics and pharmaceuticals due to its non-toxic and biodegradable properties. It has been found that plant-derived mucilage can be used as a natural thickener or emulsifier and an alternative to synthetic polymers and additives. Because it is an invisible barrier that separates the surface from the surrounding atmosphere, it is used as edible coatings to extend the shelf life of fresh vegetables and fruits as well as many food products. In addition to its functional properties, mucilage can also be used for the production of nanocarriers. In this review, we focus on mucus extraction methods and its use as a natural preservative for fresh produce. We detailed the key properties related to the extraction and preservation of food, the mechanism of the effect of mucus on the sensory properties of products, coating methods when using mucus and its recipe for preserving fruit and vegetables. Understanding the ecological, economic and scientific factors of production and the efficiency of mucus as a multi-directional agent will open up its practical application in many industries.

العلاقة: https://www.mdpi.com/2073-4360/13/7/1066Test; https://doaj.org/toc/2073-4360Test; https://doaj.org/article/1abb55506066400c95ba95e0f36d17ebTest

الإتاحة: https://doi.org/10.3390/polym13071066Test
https://doaj.org/article/1abb55506066400c95ba95e0f36d17ebTest

المؤلفون: Jianfeng Zhan, Feng He, Shuxian Chen, Abishek Jung Poudel, Ying Yang, Lin Xiao, Fu Xiang, Shiming Li

المصدر: Molecules, Vol 26, Iss 5771, p 5771 (2021)

مصطلحات موضوعية: essential oil, qiai, nanohydrogel, thermo-responsive property, antibacterial activity, Organic chemistry, QD241-441

الوصف: Essential oils (EOs) have been used in cosmetics and food due to their antimicrobial and antiviral effects. However, the applications of EOs are compromised because of their poor aqueous solubility and high volatility. Qiai ( Artemisia argyi Levl. et Van. var. argyi cv. Qiai) is a traditional Chinese herb and possesses strong antibacterial activity. Herein, we report an innovative formulation of EO as nanohydrogels, which were prepared through co-assembly of Qiai EO (QEO) and Pluronic F108 (PEG- b -PPG- b -PEG, or PF108) in aqueous solution. QEO was efficiently loaded in the PF108 micelles and formed nanohydrogels by heating the QEO/PF108 mixture solution to 37 °C, by the innate thermo-responsive property of PF108. The encapsulation efficiency and loading capacity of QEO reached 80.2% and 6.8%, respectively. QEO nanohydrogels were more stable than the free QEO with respect to volatilization. Sustained QEO release was achieved at body temperature using the QEO nanohydrogels, with the cumulative release rate reaching 95% in 35 h. In vitro antibacterial test indicated that the QEO nanohydrogels showed stronger antimicrobial activity against S. aureus and E. coli than the free QEO due to the enhanced stability and sustained-release characteristics. It has been attested that thermo-responsive QEO nanohydrogels have good potential as antibacterial cosmetics.

العلاقة: https://www.mdpi.com/1420-3049/26/19/5771Test; https://doaj.org/toc/1420-3049Test; https://doaj.org/article/eae007919ecd465097831f4deebdc7b5Test

الإتاحة: https://doi.org/10.3390/molecules26195771Test
https://doaj.org/article/eae007919ecd465097831f4deebdc7b5Test

المؤلفون: Gaëlle Levourch, Noureddine Lebaz, Abdelhamid Elaissari

المصدر: Polymers, Vol 12, Iss 1413, p 1413 (2020)

مصطلحات موضوعية: nanohydrogel particles, adsorption, complexation, metal ions, recombinant protein, Organic chemistry, QD241-441

الوصف: In biomedical diagnosis and bionanotechnologies, the extraction and purification of proteins and protein derivatives are of great interest. In fact, to purify recombinant proteins for instance, new methodologies and well appropriate material supports need to be established and also to be evaluated. In this work, hydrophilic nanohydrogel particles were prepared for recombinant proteins extraction for purification purpose. The prepared nanohydrogel polymer-based particles are hydrophilic below the volume phase transition temperature (TVPT) and dehydrated above the TVPT, due to the thermally sensitive poly(N-alkyl acrylamide) and poly(N-alkyl methacrylamide) derivatives. Then, the use of heavy metal ions in the presence of such functional particles should specifically capture recombinant proteins (i.e., proteins bearing a poly(histidine) part). In order to understand and to optimize the specific capture and the purification of recombinant proteins, various parameters have been investigated as a systematic study. Firstly, the adsorption was investigated as a function of pH and protein concentration. According to high hydration of the prepared nanohydrogel, no marked adsorption was observed. Secondly, the effect of pH was investigated and found to be the driven parameter affecting the metal ions immobilization and the recombinant proteins complexation. As a result, high protein complexation was observed at basic pH compared to non-complexation at acidic pH medium. The immobilized proteins via complexation were released by changing the pH. This decomplexation seems to be effective but depends on fixation conditions and particle surface structure.

العلاقة: https://www.mdpi.com/2073-4360/12/6/1413Test; https://doaj.org/toc/2073-4360Test; https://doaj.org/article/ddf0c9faf4504a8b905ce5912a6948f1Test

الإتاحة: https://doi.org/10.3390/polym12061413Test
https://doaj.org/article/ddf0c9faf4504a8b905ce5912a6948f1Test

المؤلفون: Hadia Baloch, Aisha Siddiqua, Asif Nawaz, Muhammad Shahid Latif, Syeda Qurbat Zahra, Suliman Yousef Alomar, Naushad Ahmad, Tarek M. Elsayed

المصدر: Gels; Volume 9; Issue 4; Pages: 284

مصطلحات موضوعية: Biomaterials, Polymers and Plastics, Organic Chemistry, Bioengineering, sulfur nanoparticles, Citrus limon, Gram-positive bacteria, Gram-negative bacteria, nanohydrogel

الوصف: The study aimed to synthesize non-noxious, clean, reliable, and green sulfur nanoparticles (SNPs) from Citrus limon leaves. The synthesized SNPs were used to analyze particle size, zeta potential, UV–visible spectroscopy, SEM, and ATR-FTIR. The prepared SNPs exhibited a globule size of 55.32 ± 2.15 nm, PDI value of 0.365 ± 0.06, and zeta potential of −12.32 ± 0.23 mV. The presence of SNPs was confirmed by UV–visible spectroscopy in the range of 290 nm. The SEM image showed that the particles were spherical with a size of 40 nm. The ATR-FTIR study showed no interaction, and all the major peaks were preserved in the formulations. An antimicrobial and antifungal study of SNPs was carried out against Gram-positive bacteria (Staph. aureus, Bacillus), Gram-negative bacteria (E. coli and Bordetella), and fungal strains (Candida albicans). The study showed that Citrus limon extract SNPs exhibited better antimicrobial and antifungal activities against Staph. aureus, Bacillus, E. coli, Bordetella, and Candida albicans at a minimal inhibitory concentration of 50 μg/mL. Different antibiotics were used alone and in combination with SNPs of Citrus limon extract to evaluate their activity against various strains of bacteria and fungal strains. The study showed that using SNPs of Citrus limon extract with antibiotics has a synergistic effect against Staph.aureus, Bacillus, E. coli, Bordetella, and Candida albicans. SNPs were embedded in nanohydrogel formulations for in vivo (wound healing) studies. In preclinical studies, SNPs of Citrus limon extract embedded within a nanohydrogel formulation (NHGF4) have shown promising results. To be widely used in clinical settings, further studies are needed to evaluate their safety and efficacy in human volunteers.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22438971d083085f70c7945960e8e352Test
https://doi.org/10.3390/gels9040284Test

المؤلفون: Abishek Jung Poudel, Shiming Li, Lin Xiao, Ying Yang, Feng He, Fu Xiang, Jianfeng Zhan, Shuxian Chen

المصدر: Molecules, Vol 26, Iss 5771, p 5771 (2021)
Molecules
Volume 26
Issue 19

مصطلحات موضوعية: Staphylococcus aureus, Artemisia argyi, Drug Compounding, Pharmaceutical Science, Organic chemistry, Poloxamer, Micelle, Article, essential oil, Analytical Chemistry, law.invention, qiai, QD241-441, antibacterial activity, law, Drug Discovery, Escherichia coli, Oils, Volatile, Physical and Theoretical Chemistry, Particle Size, Thermo responsive, Essential oil, Micelles, Aqueous solution, Microbial Viability, Chemistry, Plant Extracts, Antimicrobial, nanohydrogel, Anti-Bacterial Agents, Artemisia, Chemistry (miscellaneous), Delayed-Action Preparations, Molecular Medicine, Nanoparticles, Thermodynamics, thermo-responsive property, Antibacterial activity, Nuclear chemistry

الوصف: Essential oils (EOs) have been used in cosmetics and food due to their antimicrobial and antiviral effects. However, the applications of EOs are compromised because of their poor aqueous solubility and high volatility. Qiai (Artemisia argyi Levl. et Van. var. argyi cv. Qiai) is a traditional Chinese herb and possesses strong antibacterial activity. Herein, we report an innovative formulation of EO as nanohydrogels, which were prepared through co-assembly of Qiai EO (QEO) and Pluronic F108 (PEG-b-PPG-b-PEG, or PF108) in aqueous solution. QEO was efficiently loaded in the PF108 micelles and formed nanohydrogels by heating the QEO/PF108 mixture solution to 37 °C, by the innate thermo-responsive property of PF108. The encapsulation efficiency and loading capacity of QEO reached 80.2% and 6.8%, respectively. QEO nanohydrogels were more stable than the free QEO with respect to volatilization. Sustained QEO release was achieved at body temperature using the QEO nanohydrogels, with the cumulative release rate reaching 95% in 35 h. In vitro antibacterial test indicated that the QEO nanohydrogels showed stronger antimicrobial activity against S. aureus and E. coli than the free QEO due to the enhanced stability and sustained-release characteristics. It has been attested that thermo-responsive QEO nanohydrogels have good potential as antibacterial cosmetics.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3587bfe15d5aa6244023ffcbfd5c594bTest
https://www.mdpi.com/1420-3049/26/19/5771Test

المؤلفون: Cecilia Virginia Gheran, Sorina Nicoleta Voicu, Bianca Galateanu, Maité Callewaert, Juliette Moreau, Cyril Cadiou, Françoise Chuburu, Anca Dinischiotu

المصدر: International Journal of Molecular Sciences; Volume 23; Issue 6; Pages: 3258

مصطلحات موضوعية: Chitosan, Organic Chemistry, Contrast Media, Gadolinium, General Medicine, Magnetic Resonance Imaging, Catalysis, Computer Science Applications, Inorganic Chemistry, Mice, nanohydrogel, chitosan, contrast agents, RAW 264.7 cell line, biocompatibility, hemocompatibility, Animals, Humans, Hyaluronic Acid, Physical and Theoretical Chemistry, Molecular Biology, Spectroscopy

الوصف: The aim of this study was to investigate the biocompatibility of contrast agents, such as gadolinium 1, 4, 7, 10 tetraazacyclo-dodecane tetraacetic acid (GdDOTA) and gadolinium dioctyl terephthalate (GdDOTP), encapsulated in a polymeric matrix containing chitosan and hyaluronic acid using RAW264.7 murine macrophages and human blood samples. The cell viability and cytotoxicity were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays, while cell cycle analysis was determined in RAW264.7 cells using flow cytometry. The mitochondrial membrane potential (MMP), hemolytic index, complement activation, and thrombogenic potential of gadolinium (Gd) containing nanohydrogels were measured by fluorometric and spectrophotometric methods. Taken together, our results demonstrate the good bio- and hemocompatibility of chitosan-based nanohydrogels with the RAW264.7 cell line and human blood cells, suggesting that these could be used as injectable formulations for the magnetic resonance imaging diagnostic of lymph nodes.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e09088dca724c00cdc62d39553f90928Test
https://doi.org/10.3390/ijms23063258Test

المؤلفون: Ana I. Bourbon, Miguel A. Cerqueira, Ricardo Nuno Correia Pereira, António A. Vicente, Lorenzo Pastrana

المساهمون: Universidade do Minho

المصدر: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Gels
Gels, Vol 5, Iss 1, p 9 (2019)

مصطلحات موضوعية: 0106 biological sciences, bio-based materials, Nanostructure, Polymers and Plastics, Food industry, Bioengineering, Nanotechnology, biopolymers, Review, 01 natural sciences, lcsh:Chemistry, Biomaterials, 0404 agricultural biotechnology, lcsh:General. Including alchemy, 010608 biotechnology, lcsh:Inorganic chemistry, lcsh:Science, Science & Technology, nanotechnology, Chemistry, business.industry, Organic Chemistry, Food grade, 04 agricultural and veterinary sciences, 040401 food science, lcsh:QD146-197, nanohydrogel, lcsh:QD1-999, Food products, encapsulation, lcsh:Q, business, lcsh:QD1-65

الوصف: Proteins are receiving significant attention for the production of structures for the encapsulation of active compounds, aimed at their use in food products. Proteins are one of the most used biomaterials in the food industry due to their nutritional value, non-toxicity, biodegradability, and ability to create new textures, in particular, their ability to form gel particles that can go from macro- to nanoscale. This review points out the different techniques to obtain protein-based nanostructures and their use to encapsulate and release bioactive compounds, while also presenting some examples of food grade proteins, the mechanism of formation of the nanostructures, and the behavior under different conditions, such as in the gastrointestinal tract.
Ricardo Pereira acknowledges his Post-Doctoral grant (SFRH/BPD/81887/2011) to the Fundação para a Ciência e Tecnologia (FCT, Portugal). This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020-Programa Operacional Regional do Norte.
info:eu-repo/semantics/publishedVersion

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d8a4d0eb9a683e4ee9f5876939f730bTest