دورية أكاديمية
Development and Validation of Stability-Indicating HPLC Methods for the Estimation of Lomefloxacin and Balofloxacin Oxidation Process under ACVA, H2O2, or KMnO4 Treatment. Kinetic Evaluation and Identification of Degradation Products by Mass Spectrometry
| العنوان: | Development and Validation of Stability-Indicating HPLC Methods for the Estimation of Lomefloxacin and Balofloxacin Oxidation Process under ACVA, H2O2, or KMnO4 Treatment. Kinetic Evaluation and Identification of Degradation Products by Mass Spectrometry |
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| المؤلفون: | Barbara Żuromska-Witek, Paweł Żmudzki, Marek Szlósarczyk, Anna Maślanka, Urszula Hubicka |
| المصدر: | Molecules, Vol 25, Iss 22, p 5251 (2020) |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Organic chemistry |
| مصطلحات موضوعية: | balofloxacin, lomefloxacin, oxidation studies, kinetic evaluation, HPLC, Organic chemistry, QD241-441 |
| الوصف: | The oxidation of lomefloxacin (LOM) and balofloxacin (BAL) under the influence of azo initiator of radical reactions of 4,4′-azobis(4-cyanopentanoic acid) (ACVA) and H2O2 was examined. Oxidation using H2O2 was performed at room temperature while using ACVA at temperatures: 40, 50, 60 °C. Additionally, the oxidation process of BAL under the influence of KMnO4 in an acidic medium was investigated. New stability-indicating HPLC methods were developed in order to evaluate the oxidation process. Chromatographic analysis was carried out using the Kinetex 5u XB—C18 100A column, Phenomenex (Torrance, CA, USA) (250 × 4.6 mm, 5 μm particle size, core shell type). The chromatographic separation was achieved while using isocratic elution and a mobile phase with the composition of 0.05 M phosphate buffer (pH = 3.20 adjusted with o-phosphoric acid) and acetonitrile (87:13 v/v for LOM; 80:20 v/v for BAL). The column was maintained at 30 °C. The methods were validated according to the ICH guidelines, and it was found that they met the acceptance criteria. An oxidation process followed kinetics of the second order reaction. The most probable structures of LOM and BAL degradation products formed were assigned by the UHPLC/MS/MS method. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 25225251 1420-3049 |
| العلاقة: | https://www.mdpi.com/1420-3049/25/22/5251Test; https://doaj.org/toc/1420-3049Test |
| DOI: | 10.3390/molecules25225251 |
| الوصول الحر: | https://doaj.org/article/e1b00217738f46d791d9958ebbaa628fTest |
| رقم الانضمام: | edsdoj.1b00217738f46d791d9958ebbaa628f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25225251 14203049 |
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| DOI: | 10.3390/molecules25225251 |