التفاصيل البيبلوغرافية
| العنوان: |
TERT Promoter Mutations as Simple and Non-Invasive Urinary Biomarkers for the Detection of Urothelial Bladder Cancer in a High-Risk Region. |
| المؤلفون: |
Pakmanesh, Hamid, Anvari, Omid, Forey, Nathalie, Weiderpass, Elisabete, Malekpourafshar, Reza, Iranpour, Maryam, Shahesmaeili, Armita, Ahmadi, Nahid, Bazrafshan, Azam, Zendehdel, Kazem, Kannengiesser, Caroline, Ba, Ibrahima, McKay, James, Zvereva, Maria, Hosen, Md Ismail, Sheikh, Mahdi, Calvez-Kelm, Florence Le |
| المصدر: |
International Journal of Molecular Sciences; Nov2022, Vol. 23 Issue 22, p14319, 13p |
| مصطلحات موضوعية: |
BLADDER cancer, TELOMERASE reverse transcriptase, TRANSITIONAL cell carcinoma, SOMATIC mutation, GENETIC mutation |
| مصطلحات جغرافية: |
IRAN |
| مستخلص: |
Bladder cancer (BC) is the 10th most common cancer in the world. While there are FDA-approved urinary assays to detect BC, none have demonstrated sufficient sensitivity and specificity to be integrated into clinical practice. Telomerase Reverse Transcriptase (TERT) gene mutations have been identified as the most common BC mutations that could potentially be used as non-invasive urinary biomarkers to detect BC. This study aims to evaluate the validity of these tests to detect BC in the Kerman province of Iran, where BC is the most common cancer in men. Urine samples of 31 patients with primary (n = 11) or recurrent (n = 20) bladder tumor and 50 controls were prospectively collected. Total urinary DNA was screened for the TERT promoter mutations (uTERTpm) by Droplet Digital PCR (ddPCR) assays. The performance characteristics of uTERTpm and the influence by disease stage and grade were compared to urine cytology results. The uTERTpm was 100% sensitive and 88% specific to detect primary BC, while it was 50% sensitive and 88% specific in detecting recurrent BC. The overall sensitivity and specificity of uTERTpm to detect bladder cancer were 67.7% and 88.0%, respectively, which were consistent across different tumor stages and grades. The most frequent uTERTpm mutations among BC cases were C228T (18/31), C250T (4/31), and C158A (1/31) with mutant allelic frequency (MAF) ranging from 0.2% to 63.3%. Urine cytology demonstrated a similar sensitivity (67.7%), but lower specificity (62.0%) than uTERTpm in detecting BC. Combined uTERTpm and urine cytology increased the sensitivity to 83.8%, but decreased the specificity to 52.0%. Our study demonstrated promising diagnostic accuracy for the uTERTpm as a non-invasive urinary biomarker to detect, in particular, primary BC in this population. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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