دورية أكاديمية
Colistin resistance in Escherichia coli and Klebsiella pneumoniae in humans and backyard animals in Ecuador
| العنوان: | Colistin resistance in Escherichia coli and Klebsiella pneumoniae in humans and backyard animals in Ecuador |
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| المؤلفون: | Carlos Bastidas-Caldes, Salomé Guerrero-Freire, Nimer Ortuño-Gutiérrez, Temmy Sunyoto, Cícero Armídio Gomes-Dias, Maria Soledad Ramírez, William Calero-Cáceres, Anthony D. Harries, Joaquín Rey, Jacobus H. de Waard, Manuel Calvopiña |
| المصدر: | Revista Panamericana de Salud Pública, Vol 47, Iss 48, Pp 1-8 (2023) |
| بيانات النشر: | Pan American Health Organization, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine LCC:Arctic medicine. Tropical medicine LCC:Public aspects of medicine |
| مصطلحات موضوعية: | colistin, escherichia coli, klebsiella pneumoniae, humans, animals, drug resistance, genes, mdr, operational research, ecuador, Medicine, Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270 |
| الوصف: | Objective. Colistin is an antibiotic of last resort for treating serious Gram-negative bacterial infections. However, the misuse of colistin, especially as an animal growth promoter, has contributed to increasing antimicrobial resistance, mediated mainly through plasmid transfer of the mcr-1 gene. This study assessed the prevalence of phenotypic and molecular colistin resistance in Escherichia coli and Klebsiella pneumoniae in Ecuador in healthy humans and their chickens and pigs. Methods. Fecal samples were collected from humans and their chickens and pigs in two rural coastal and Amazon regions between April and August 2020. Gram-negative bacteria were isolated and identified using conventional techniques. Phenotypic resistance was determined using the broth microdilution technique, and the mcr-1 gene was detected using conventional polymerase chain reaction. Results. A total of 438 fecal samples were obtained from 137 humans, 147 pigs and 154 chickens. The prevalence of E. coli isolates was 86.3% (378/438) and K. pneumoniae, 37.4% (164/438). Overall, the mcr-1 gene was found in 90% (340/378) of E. coli isolates, with higher prevalences found in isolates from coastal regions (96.5%, 191/198), humans (95.6%, 111/116) and chickens (91.8%, 123/134); for K. pneumoniae, the gene was found in 19.5% (32/164) of isolates, with equal distribution between regions and hosts. Only four isolates, two E. coli and two K. pneumoniae, showed phenotypic resistance: mcr-1 was present in both E. coli strains but absent in the K. pneumoniae strains. Conclusions. Despite a low prevalence of phenotypic resistance to colistin, the high prevalence of the mcr-1 gene in E. coli is of concern. Ecuador’s ban on using colistin in animal husbandry must be enforced, and continual monitoring of the situation should be implemented. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Spanish; Castilian Portuguese |
| تدمد: | 1020-4989 1680-5348 |
| العلاقة: | https://iris.paho.org/handle/10665.2/57328Test; https://doaj.org/toc/1020-4989Test; https://doaj.org/toc/1680-5348Test |
| DOI: | 10.26633/RPSP.2023.48 |
| الوصول الحر: | https://doaj.org/article/8d5db3468bff439f951cc2619a57071eTest |
| رقم الانضمام: | edsdoj.8d5db3468bff439f951cc2619a57071e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 10204989 16805348 |
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| DOI: | 10.26633/RPSP.2023.48 |