التفاصيل البيبلوغرافية
| العنوان: |
DNA probe detection of periodontal pathogens. |
| المؤلفون: |
French, C. K., Savitt, E. D., Simon, S. L., Eklund, S. M., Chen, M. C., Klotz, L. C., Vaccaro, K. K. |
| المصدر: |
Oral Microbiology & Immunology; Nov1986, Vol. 1 Issue 1, p58-62, 5p |
| مصطلحات موضوعية: |
DNA, NUCLEIC acids, ACTINOBACILLUS, BACTEROIDES, PATHOGENIC microorganisms, GINGIVA |
| مستخلص: |
The technology for hybridization of nucleic acids using isotopically labeled whole genomic or species-specific cloned deoxyribonucleic acid (DNA) probes for Actinobacillus actinomycetemcomitans, Bacteroides intermedius and Bacteroides gingivalis can be used to detect as few as 10² cells of these periodontal pathogens. The sensitivity and specificity of the test is not affected by the presence of 5 × 105 unrelated bacteria in constructed mixed culture samples. Current feasibility tests with non-isotopic reagents also give reliable and rapid detection of these oral pathogens in pure or mixed cultures. DNA probes should prove useful in a rapid, specific, and sensitive assay for these periodontal pathogens. [ABSTRACT FROM AUTHOR] |
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Copyright of Oral Microbiology & Immunology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
