التفاصيل البيبلوغرافية
| العنوان: |
Efficient Regeneration of Human Vα24+ Invariant Natural Killer T Cells and Their Anti-Tumor Activity In Vivo. |
| المؤلفون: |
Yamada, Daisuke, Iyoda, Tomonori, Vizcardo, Raul, Shimizu, Kanako, Sato, Yusuke, Endo, Takaho A., Kitahara, Genta, Okoshi, Momoko, Kobayashi, Midori, Sakurai, Maki, Ohara, Osamu, Taniguchi, Masaru, Koseki, Haruhiko, Fujii, Shin-ichiro |
| المصدر: |
Stem Cells; Dec2016, Vol. 34 Issue 12, p2852-2860, 9p |
| مستخلص: |
A bstract Reprogramming of antigen-specific T lymphocytes into induced pluripotent stem cells (iPSCs) and their subsequent re-differentiation has enabled expansion of functional T lymphocytes in vitro, thus opening up new approaches for immunotherapy of cancer and other diseases. In this study, we have established a robust protocol to reprogram human invariant NKT (Vα24+ iNKT) cells, which have been shown to act as cellular adjuvants and thus exert anti-tumor activity in mice and humans, and to re-differentiate the iNKT cell-derived iPSCs into functional iNKT cells. These iPSC-derived iNKT cells (iPS-Vα24+ iNKT cells) can be activated by ligand-pulsed dendritic cells (DCs) and produce a large amount of interferon-γ upon activation, as much as parental Vα24+ iNKT cells, but exhibit even better cytotoxic activity against various tumor cell lines. The iPS-Vα24+ iNKT cells possess significant anti-tumor activity in tumor-bearing mice and can activate autologous NK cells upon activation by ligand-pulsed DCs in the NOG mouse model in vivo, further extending their therapeutic potential. This study thus provides a first proof of concept for the clinical application of human iPS-Vα24+ iNKT cells for cancer immunotherapy. S tem C ells 2016;34:2852-2860 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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