التفاصيل البيبلوغرافية
| العنوان: |
Cloning and characterization of novel snake venom proteins that block smooth muscle contraction. |
| المؤلفون: |
Yamazaki, Yasuo, Koike, Hisashi, Sugiyama, Yusuke, Motoyoshi, Kazuko, Wada, Taeko, Hishinuma, Shigeru, Mita, Mitsuo, Morita, Takashi |
| المصدر: |
European Journal of Biochemistry; Jun2002, Vol. 269 Issue 11, p2708-2715, 8p |
| مصطلحات موضوعية: |
SNAKE venom, NEUROTOXIC agents, CLONING |
| مستخلص: |
In this study, we isolated a 25-kDa novel snake venom protein, designated ablomin, from the venom of the Japanese Mamushi snake (Agkistrodon blomhoffi ). The amino-acid sequence of this protein was determined by peptide sequencing and cDNA cloning. The deduced sequence showed high similarity to helothermine from the Mexican beaded lizard (Heloderma horridum horridum ), which blocks voltage-gated calcium and potassium channels, and ryanodine receptors. Ablomin blocked contraction of rat tail arterial smooth muscle elicited by high K+ -induced depolarization in the 0.1–1 µm range, but did not block caffeine-stimulated contraction. Furthermore, we isolated three other proteins from snake venoms that are homologous to ablomin and cloned the corresponding cDNAs. Two of these homologous proteins, triflin and latisemin, also inhibited high K+ -induced contraction of the artery. These results indicate that several snake venoms contain novel proteins with neurotoxin-like activity. [ABSTRACT FROM AUTHOR] |
|
Copyright of European Journal of Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder