دورية أكاديمية
Munc18-1 is essential for neuropeptide secretion in neurons
| العنوان: | Munc18-1 is essential for neuropeptide secretion in neurons |
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| المؤلفون: | Puntman, Daniël C., Arora, Swati, Farina, Margherita, Toonen, Ruud F., Verhage, Matthijs |
| المصدر: | Puntman , D C , Arora , S , Farina , M , Toonen , R F & Verhage , M 2021 , ' Munc18-1 is essential for neuropeptide secretion in neurons ' , Journal of Neuroscience , vol. 41 , no. 28 , pp. 5980-5993 . https://doi.org/10.1523/JNEUROSCI.3150-20.2021Test |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Dense-core vesicle, Munc18-1, Neuropeptide |
| الوصف: | Neuropeptide secretion from dense-core vesicles (DCVs) controls many brain functions. Several components of the DCV exocytosis machinery have recently been identified, but the participation of a SEC1/MUNC18 (SM) protein has remained elusive. Here, we tested the ability of the three exocytic SM proteins expressed in the mammalian brain, MUNC18-1/2/3, to support neuropeptide secretion. We quantified DCV exocytosis at a single vesicle resolution on action potential (AP) train-stimulation in mouse CNS neurons (of unknown sex) using pHluorin-tagged and/or mCherry-tagged neuropeptide Y (NPY) or brain-derived neurotrophic factor (BDNF). Conditional inactivation of Munc18-1 abolished all DCV exocytosis. Expression of MUNC18-1, but not MUNC18-2 or MUNC18-3, supported DCV exocytosis in Munc18-1 null neurons. Heterozygous (HZ) inactivation of Munc18-1, as a model for reduced MUNC18-1 expression, impaired DCV exocytosis, especially during the initial phase of train-stimulation, when the release was maximal. These data show that neurons critically and selectively depend on MUNC18-1 for neuropeptide secretion. Impaired neuropeptide secretion may explain aspects of the behavioral and neurodevelopmental phenotypes that were observed in Munc18-1 HZ mice. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://research.vu.nl/en/publications/4c0f2947-ec1f-45f5-9d1f-cc9356c5b615Test |
| DOI: | 10.1523/JNEUROSCI.3150-20.2021 |
| الإتاحة: | https://doi.org/10.1523/JNEUROSCI.3150-20.2021Test https://research.vu.nl/en/publications/4c0f2947-ec1f-45f5-9d1f-cc9356c5b615Test https://hdl.handle.net/1871.1/4c0f2947-ec1f-45f5-9d1f-cc9356c5b615Test https://research.vu.nl/ws/files/153314125/Munc18_1_is_essential_for_neuropeptide_secretion_in_neurons.pdfTest http://www.scopus.com/inward/record.url?scp=85111494998&partnerID=8YFLogxKTest http://www.scopus.com/inward/citedby.url?scp=85111494998&partnerID=8YFLogxKTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.701D4B6D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1523/JNEUROSCI.3150-20.2021 |
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