التفاصيل البيبلوغرافية
| العنوان: |
Spectrally-Resolved Response Properties of the Three Most Advanced FRET Based Fluorescent Protein Voltage Probes. |
| المؤلفون: |
Mutoh, Hiroki, Perron, Amelie, Dimitrov, Dimitar, Iwamoto, Yuka, Akemann, Walther, Chudakov, Dmitriy M., Knöpfel, Thomas |
| المصدر: |
PLoS ONE; 2009, Vol. 4 Issue 2, p1-5, 5p, 2 Diagrams, 1 Chart, 1 Graph |
| مصطلحات موضوعية: |
FLUORESCENT probes, AMINO acid sequence, IMMUNE response, NEURAL transmission, ANALYSIS of variance, AMINO acids |
| مستخلص: |
Genetically-encoded optical probes for membrane potential hold the promise of monitoring electrical signaling of electrically active cells such as specific neuronal populations in intact brain tissue. The most advanced class of these probes was generated by molecular fusion of the voltage sensing domain (VSD) of Ci-VSP with a fluorescent protein (FP) pair. We quantitatively compared the three most advanced versions of these probes (two previously reported and one new variant), each involving a spectrally distinct tandemof FPs. Despite these different FP tandems and dissimilarities within the amino acid sequence linking the VSD to the FPs, the amplitude and kinetics of voltage dependent fluorescence changes were surprisingly similar. However, each of these fluorescent probes has specific merits when considering different potential applications. [ABSTRACT FROM AUTHOR] |
|
Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder