دورية أكاديمية
New insights into extracellular and intracellular redox status in COVID-19 patients
| العنوان: | New insights into extracellular and intracellular redox status in COVID-19 patients |
|---|---|
| المؤلفون: | Mohammad Javad Tavassolifar, Hamid Asadzadeh Aghdaei, Omid Sadatpour, Samaneh Maleknia, Sara Fayazzadeh, Seyed Reza Mohebbi, Fatemeh Montazer, Amirhassan Rabbani, Mohammad Reza Zali, Maryam Izad, Anna Meyfour |
| المصدر: | Redox Biology, Vol 59, Iss , Pp 102563- (2023) |
| بيانات النشر: | Elsevier, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | SARS-COV-2, Redox system, Nasopharyngeal, PBMCs, Immune system, COVID-19, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | Background: The imbalance of redox homeostasis induces hyper-inflammation in viral infections. In this study, we explored the redox system signature in response to SARS-COV-2 infection and examined the status of these extracellular and intracellular signatures in COVID-19 patients. Method: The multi-level network was constructed using multi-level data of oxidative stress-related biological processes, protein-protein interactions, transcription factors, and co-expression coefficients obtained from GSE164805, which included gene expression profiles of peripheral blood mononuclear cells (PBMCs) from COVID-19 patients and healthy controls. Top genes were designated based on the degree and closeness centralities. The expression of high-ranked genes was evaluated in PBMCs and nasopharyngeal (NP) samples of 30 COVID-19 patients and 30 healthy controls. The intracellular levels of GSH and ROS/O2• − and extracellular oxidative stress markers were assayed in PBMCs and plasma samples by flow cytometry and ELISA. ELISA results were applied to construct a classification model using logistic regression to differentiate COVID-19 patients from healthy controls. Results: CAT, NFE2L2, SOD1, SOD2 and CYBB were 5 top genes in the network analysis. The expression of these genes and intracellular levels of ROS/O2• − were increased in PBMCs of COVID-19 patients while the GSH level decreased. The expression of high-ranked genes was lower in NP samples of COVID-19 patients compared to control group. The activity of extracellular enzymes CAT and SOD, and the total oxidant status (TOS) level were increased in plasma samples of COVID-19 patients. Also, the 2-marker panel of CAT and TOS and 3-marker panel showed the best performance. Conclusion: SARS-COV-2 disrupts the redox equilibrium in immune cells and the upper respiratory tract, leading to exacerbated inflammation and increased replication and entrance of SARS-COV-2 into host cells. Furthermore, utilizing markers of oxidative stress as a complementary validation to discriminate COVID-19 from healthy controls, seems promising. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2213-2317 |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S2213231722003354Test; https://doaj.org/toc/2213-2317Test |
| DOI: | 10.1016/j.redox.2022.102563 |
| الوصول الحر: | https://doaj.org/article/3a9ae7547dbc458fb34d2335049e2654Test |
| رقم الانضمام: | edsdoj.3a9ae7547dbc458fb34d2335049e2654 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22132317 |
|---|---|
| DOI: | 10.1016/j.redox.2022.102563 |