التفاصيل البيبلوغرافية
| العنوان: |
Sol–gel nanoporous silica as substrate for immobilization of conjugated biomolecules for application as fluorescence resonance energy transfer (FRET) based biosensor. |
| المؤلفون: |
Nampi, Padmaja Parameswaran1 padmavasudev@gmail.com, Kartha, Cheranellore Chandrasekharan2, Jose, Gin3, P.R., Anil Kumar1, Anilkumar, Thapasimuthu1, Varma, Harikrishna1 |
| المصدر: |
Sensors & Actuators B: Chemical. Aug2013, Vol. 185, p252-257. 6p. |
| مصطلحات موضوعية: |
*SOL-gel processes, *NANOPOROUS materials, *SILICA, *BIOMOLECULES, *FLUORESCENCE resonance energy transfer, *BIOSENSORS |
| مستخلص: |
Highlights: [•] Nanoporous sol–gel silica beads can be used as a substrate for FRET-based biosensing. [•] Silica is converted into biosensor by functionalization and crosslinking Cy3-antibody to which Cy5-Protein A is anchored. [•] Fluorescence property has been utilized to evaluate the potential of this biosensor to detect troponin. [•] Results of this research show that the detection limit of troponin using the FRET system is 10–5ngmL−1. [•] Optimized parameters considering the detection limits is needed for extending the method for detection of other biomarkers. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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