دورية أكاديمية
Immunogenicity and Protective Potential of Mucosal Vaccine Formulations Based on Conserved Epitopes of Influenza A Viruses Fused to an Innovative Ring Nanoplatform in Mice and Chickens
| العنوان: | Immunogenicity and Protective Potential of Mucosal Vaccine Formulations Based on Conserved Epitopes of Influenza A Viruses Fused to an Innovative Ring Nanoplatform in Mice and Chickens |
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| المؤلفون: | Calzas, Cynthia, Mao, Molida, Turpaud, Mathilde, Viboud, Quentin, Mettier, Joelle, Figueroa, Thomas, Bessière, Pierre, Mangin, Antoine, Sedano, Laura, Hervé, Pierre-Louis, Volmer, Romain, Ducatez, Mariette, F, Bourgault, Steve, Archambault, Denis, Le Goffic, Ronan, Chevalier, Christophe |
| المساهمون: | Virologie et Immunologie Moléculaires (VIM (UR 0892)), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Environnement, Ville, Société (EVS), École normale supérieure de Lyon (ENS de Lyon)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-Université Lumière - Lyon 2 (UL2)-Université Jean Moulin - Lyon 3 (UJML), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet - Saint-Étienne (UJM)-École Nationale des Travaux Publics de l'État (ENTPE)-École nationale supérieure d'architecture de Lyon (ENSAL)-Centre National de la Recherche Scientifique (CNRS), Cardiff University, Université du Québec à Montréal = University of Québec in Montréal (UQAM) |
| المصدر: | ISSN: 1664-3224. |
| بيانات النشر: | HAL CCSD Frontiers |
| سنة النشر: | 2021 |
| المجموعة: | Université de Versailles Saint-Quentin-en-Yvelines: HAL-UVSQ |
| مصطلحات موضوعية: | influenza A viruses, highly pathogenic avian influenza virus, mucosal vaccines, adjuvants, nanoparticles, M2e/HA2 subunit vaccines, [SDV.IMM.VAC]Life Sciences [q-bio]/Immunology/Vaccinology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| الوصف: | International audience ; Current inactivated vaccines against influenza A viruses (IAV) mainly induce immune responses against highly variable epitopes across strains and are mostly delivered parenterally, limiting the development of an effective mucosal immunity. In this study, we evaluated the potential of intranasal formulations incorporating conserved IAV epitopes, namely the long alpha helix (LAH) of the stalk domain of hemagglutinin and three tandem repeats of the ectodomain of the matrix protein 2 (3M2e), as universal mucosal anti-IAV vaccines in mice and chickens. The IAV epitopes were grafted to nanorings, a novel platform technology for mucosal vaccination formed by the nucleoprotein (N) of the respiratory syncytial virus, in fusion or not with the C-terminal end of the P97 protein (P97c), a recently identified Toll-like receptor 5 agonist. Fusion of LAH to nanorings boosted the generation of LAH-specific systemic and local antibody responses as well as cellular immunity in mice, whereas the carrier effect of nanorings was less pronounced towards 3M2e. Mice vaccinated with chimeric nanorings bearing IAV epitopes in fusion with P97c presented modest LAH- or M2e-specific IgG titers in serum and were unable to generate a mucosal humoral response. In contrast, N-3M2e or N-LAH nanorings admixed with Montanide™ gel (MG) triggered strong specific humoral responses, composed of serum type 1/type 2 IgG and mucosal IgG and IgA, as well as cellular responses dominated by type 1/type 17 cytokine profiles. All mice vaccinated with the [N-3M2e + N-LAH + MG] formulation survived an H1N1 challenge and the combination of both N-3M2e and N-LAH nanorings with MG enhanced the clinical and/or virological protective potential of the preparation in comparison to individual nanorings. Chickens vaccinated parenterally or mucosally with N-LAH and N-3M2e nanorings admixed with Montanide™ adjuvantsdeveloped a specific systemic humoral response, which nonetheless failed to confer protection against heterosubtypic challenge with a ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-03575999; https://hal.inrae.fr/hal-03575999Test; https://hal.inrae.fr/hal-03575999/documentTest; https://hal.inrae.fr/hal-03575999/file/CALZAS-2021-FrontiersImmuno.pdfTest; PUBMEDCENTRAL: PMC8632632 |
| DOI: | 10.3389/fimmu.2021.772550 |
| الإتاحة: | https://doi.org/10.3389/fimmu.2021.772550Test https://hal.inrae.fr/hal-03575999Test https://hal.inrae.fr/hal-03575999/documentTest https://hal.inrae.fr/hal-03575999/file/CALZAS-2021-FrontiersImmuno.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.F5FABA4C |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fimmu.2021.772550 |
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