Curcumin and Ethanol Effects in Trembler-J Schwann Cell Culture
| العنوان: | Curcumin and Ethanol Effects in Trembler-J Schwann Cell Culture |
|---|---|
| المؤلفون: | Lucia Vázquez Alberdi, Gonzalo Rosso, Lucía Velóz, Carlos Romeo, Joaquina Farias, María Vittoria Di Tomaso, Miguel Calero, Alejandra Kun |
| المساهمون: | Ministerio de Ciencia e Innovación (España), Centro de Investigación Biomédica en Red - CIBERNED (Enfermedades Neurodegenerativas), University of the Republic (Uruguay), Agencia Nacional de Investigación e Innovación (Uruguay) |
| المصدر: | Biomolecules; Volume 12; Issue 4; Pages: 515 Repisalud Instituto de Salud Carlos III (ISCIII) |
| بيانات النشر: | Preprints, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Mammals, Curcumin, Hsps, Ethanol, Cell Culture Techniques, Trembler-J, Biochemistry, Mice, cell_developmental_biology, Charcot-Marie-Tooth Disease, CMT1E, Autophagy, Animals, curcumin, ethanol, autophagy, Molecular Biology, Myelin Proteins |
| الوصف: | Charcot-Marie-Tooth (CMT) syndrome is the most common progressive human motor and sensory peripheral neuropathy. CMT type 1E is a demyelinating neuropathy affecting Schwann cells due to peripheral-myelin-protein-22 (PMP22) mutations, modelized by Trembler-J mice. Curcumin, a natural polyphenol compound obtained from turmeric (Curcuma longa), exhibits dose- and time-varying antitumor, antioxidant and neuroprotective properties, however, the neurotherapeutic actions of curcumin remain elusive. Here, we propose curcumin as a possible natural treatment capable of enhancing cellular detoxification mechanisms, resulting in an improvement of the neurodegenerative Trembler-J phenotype. Using a refined method for obtaining enriched Schwann cell cultures, we evaluated the neurotherapeutic action of low dose curcumin treatment on the PMP22 expression, and on the chaperones and autophagy/mammalian target of rapamycin (mTOR) pathways in Trembler-J and wild-type genotypes. In wild-type Schwann cells, the action of curcumin resulted in strong stimulation of the chaperone and macroautophagy pathway, whereas the modulation of ribophagy showed a mild effect. However, despite the promising neuroprotective effects for the treatment of neurological diseases, we demonstrate that the action of curcumin in Trembler-J Schwann cells could be impaired due to the irreversible impact of ethanol used as a common curcumin vehicle necessary for administration. These results contribute to expanding our still limited understanding of PMP22 biology in neurobiology and expose the intrinsic lability of the neurodegenerative Trembler-J genotype. Furthermore, they unravel interesting physiological mechanisms of cellular resilience relevant to the pharmacological treatment of the neurodegenerative Tremble J phenotype with curcumin and ethanol. We conclude that the analysis of the effects of the vehicle itself is an essential and inescapable step to comprehensibly assess the effects and full potential of curcumin treatment for therapeutic purposes. TThis research was funded by the Comisión Sectorial de Investigación Científica de la Universidad de la República (CSIC I+D, 2013), Agencia Nacional de Investigación e Innovación (ANII, FCE_1_2019_1_155539) and Programa de Desarrollo de Ciencias Básicas (PEDECIBA, CCA-Bio/Res. 6.1-2/4/2019). This work was also supported by a grant PID2019-110401RB-100 from the Spanish, Ministry of Science and Innovation and the Spanish CIBERNED network (M.C.). Sí |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb55b3c11d71e0c270b58b267b15a758Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....cb55b3c11d71e0c270b58b267b15a758 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |