Molecular characterization of rpoB gene mutations in isolates from tuberculosis patients in Cubal, Republic of Angola
| العنوان: | Molecular characterization of rpoB gene mutations in isolates from tuberculosis patients in Cubal, Republic of Angola |
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| المؤلفون: | Maria Teresa López García, Josep Vegue Collado, Tomas Pumarola Suñe, Adriano Zacarias, Cristina Bocanegra Garcia, María Luisa Aznar, Mateu Espasa Soley, Estevao Gabriel, Israel Molina Romero, María Milagros Moreno, Domingos Katimba, M. T. Fernandez, Elena Sulleiro Igual, Arlete Nindia Eugénio, Ariadna Rando-Segura, Carlos Escartin Huesca, Maria Carmen Vivas Cano, Eva Gil Olivas |
| المساهمون: | Institut Català de la Salut, [Rando-Segura A, Espasa Soley M, Sulleiro Igual E, Escartin Huesca C, Vegue Collado J, Vivas Cano MC, Pumarola Suñe T, Tórtola Fernández MT] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. PROSICS Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Aznar ML, Bocanegra Garcia C, Gil Olivas E] Servei de Malalties Infeccioses, Vall d’Hebron Hospital Universitari, Barcelona, Spain. PROSICS Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Hospital Nossa Senhora da Paz, Cubal, Angola. [Moreno MM] Hospital Nossa Senhora da Paz, Cubal, Angola. [Molina Romero I] Servei de Malalties Infeccioses, Vall d’Hebron Hospital Universitari, Barcelona, Spain. PROSICS Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain, Vall d'Hebron Barcelona Hospital Campus |
| المصدر: | Scientia BMC Infectious Diseases BMC Infectious Diseases, Vol 21, Iss 1, Pp 1-7 (2021) |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Tuberculosis, Cubal, Resistance, rpoB mutations, Infectious and parasitic diseases, RC109-216, Microbial Sensitivity Tests, Gene mutation, medicine.disease_cause, Mycobacterium tuberculosis, Bacterial Proteins, Drug Resistance, Bacterial, Genotype, polycyclic compounds, medicine, Humans, enzimas y coenzimas::enzimas::transferasas::fosfotransferasas::nucleotidiltransferasas::ARN nucleotidiltransferasas::ARN polimerasas dirigidas por ADN [COMPUESTOS QUÍMICOS Y DROGAS], Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Tuberculosis [DISEASES], Rifampicin, Enzymes and Coenzymes::Enzymes::Transferases::Phosphotransferases::Nucleotidyltransferases::RNA Nucleotidyltransferases::DNA-Directed RNA Polymerases [CHEMICALS AND DRUGS], Tuberculosi - Epidemiologia, Mutation, biology, Research, DNA-Directed RNA Polymerases, GenoType MTBDRplus VER2.0, bacterial infections and mycoses, biology.organism_classification, rpoB, medicine.disease, Virology, Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Microbial Sensitivity Tests [ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT], RNA-polimerases, Infectious Diseases, Angola, infecciones bacterianas y micosis::infecciones bacterianas::infecciones por bacterias grampositivas::infecciones por Actinomycetales::micobacteriosis::tuberculosis [ENFERMEDADES], Parasitology, Tests de sensibilitat microbiològica, diagnóstico::técnicas y procedimientos diagnósticos::técnicas de laboratorio clínico::técnicas microbiológicas::pruebas de sensibilidad microbiana [TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS], medicine.drug |
| الوصف: | Background The importance of Mycobacterium tuberculosis strains with disputed rpoB mutations remains to be defined. This study aimed to assess the frequency and types of rpoB mutations in M. tuberculosis isolates from Cubal, Angola, a country with a high incidence of tuberculosis. Methods All isolates included (n = 308) were analyzed using phenotypic drug susceptibility testing and GenoType MTBDRplus assay. DNA sequencing of the rpoB gene and determination of rifampicin MIC by macrodilution method were additionally performed on isolates yielding discordant results (n = 12) and those in which the mutation detected was not characterized (n = 8). Results In total, 85.1% (74/87) of rifampicin-resistant strains had undisputed rpoB mutations -S450L (49), D435V (15), H445D (3), H445Y (2), Q432ins (1), L449M plus S450F (1), S450F (1), S450W (1) and S450Y (1)-; 10.3% (9/87) had disputed rpoB mutations—L430P plus S493L (1), N437del (1), H445L (3), D435Y (2), L452P (2)-, 2.3% (2.3%) showed no rpoB mutations and 2.3% (2/87) showed heteroresistance—D435Y plus L452P and L430P plus S493L-. Conclusion Disputed rpoB mutations were common, occurring in 10.3% of rifampicin resistant isolates. Current phenotyping techniques may be unable to detect this resistance pattern. To increase their sensitivity, a lower concentration of RIF could be used in these tests or alternatively, rpoB mutations could be screened and characterized in all M. tuberculosis strains. |
| وصف الملف: | application/pdf |
| تدمد: | 1471-2334 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1dc5a7d2fc43fb87655674df5088cfacTest https://doi.org/10.1186/s12879-021-06763-8Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1dc5a7d2fc43fb87655674df5088cfac |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712334 |
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