دورية أكاديمية
Multimodal nonlinear microscope based on a compact fiber-format laser source
| العنوان: | Multimodal nonlinear microscope based on a compact fiber-format laser source |
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| المؤلفون: | Crisafi, Francesco, Kumar, Vikas, Perri, Antonio, Marangoni, Marco, Cerullo, Giulio, Polli, Dario |
| المساهمون: | Crisafi, Francesco, Kumar, Vika, Perri, Antonio, Marangoni, Marco, Cerullo, Giulio, Polli, Dario |
| سنة النشر: | 2018 |
| المجموعة: | RE.PUBLIC@POLIMI - Research Publications at Politecnico di Milano |
| مصطلحات موضوعية: | CARS, Coherent Raman spectroscopy, Multimodal, Nonlinear optical microscopy, SRS, TPEF |
| الوصف: | We present a multimodal non-linear optical (NLO) laser-scanning microscope, based on a compact fiber-format excitation laser and integrating coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS) and two-photon-excitation fluorescence (TPEF) on a single platform. We demonstrate its capabilities in simultaneously acquiring CARS and SRS images of a blend of 6-μm poly(methyl methacrylate) beads and 3-μm polystyrene beads. We then apply it to visualize cell walls and chloroplast of an unprocessed fresh leaf of Elodea aquatic plant via SRS and TPEF modalities, respectively. The presented NLO microscope, developed in house using off-the-shelf components, offers full accessibility to the optical path and ensures its easy re-configurability and flexibility. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/28709138; info:eu-repo/semantics/altIdentifier/wos/WOS:000412608600019; volume:188; firstpage:135; lastpage:140; numberofpages:6; journal:SPECTROCHIMICA ACTA. PART A, MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; info:eu-repo/grantAgreement/EC/H2020/648615, 291198; http://hdl.handle.net/11311/1063826Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85021889014 |
| DOI: | 10.1016/j.saa.2017.06.055 |
| الإتاحة: | https://doi.org/10.1016/j.saa.2017.06.055Test http://hdl.handle.net/11311/1063826Test |
| رقم الانضمام: | edsbas.D5798250 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.saa.2017.06.055 |
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