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1دورية أكاديمية
المؤلفون: Kang-Chi Wu, Kuo-Feng Hua, Yu-Hsiang Yu, Yeong-Hsiang Cheng, Ting-Ting Cheng, Yao-Kuan Huang, Hui-Wen Chang, Wei-Jung Chen
المصدر: International Journal of Molecular Sciences; Volume 22; Issue 8; Pages: 3926
مصطلحات موضوعية: enterotoxigenic Escherichia coli (ETEC), antimicrobial peptides (AMPs), multidrug resistance (MDR), antibiofilm, intestinal porcine epithelial cell-1 (IPEC-1)
جغرافية الموضوع: agris
الوصف: Post-weaning diarrhea due to enterotoxigenic Escherichia coli (ETEC) is a common disease of piglets and causes great economic loss for the swine industry. Over the past few decades, decreasing effectiveness of conventional antibiotics has caused serious problems because of the growing emergence of multidrug-resistant (MDR) pathogens. Various studies have indicated that antimicrobial peptides (AMPs) have potential to serve as an alternative to antibiotics owing to rapid killing action and highly selective toxicity. Our previous studies have shown that AMP GW-Q4 and its derivatives possess effective antibacterial activities against the Gram-negative bacteria. Hence, in the current study, we evaluated the antibacterial efficacy of GW-Q4 and its derivatives against MDR ETEC and their minimal inhibition concentration (MIC) values were determined to be around 2~32 μg/mL. Among them, AMP Q4-15a-1 with the second lowest MIC (4 μg/mL) and the highest minimal hemolysis concentration (MHC, 256 μg/mL), thus showing the greatest selectivity (MHC/MIC = 64) was selected for further investigations. Moreover, Q4-15a-1 showed dose-dependent bactericidal activity against MDR ETEC in time–kill curve assays. According to the cellular localization and membrane integrity analyses using confocal microscopy, Q4-15a-1 can rapidly interact with the bacterial surface, disrupt the membrane and enter cytosol in less than 30 min. Minimum biofilm eradication concentration (MBEC) of Q4-15a-1 is 4× MIC (16 μg/mL), indicating that Q4-15a-1 is effective against MDR ETEC biofilm. Besides, we established an MDR ETEC infection model with intestinal porcine epithelial cell-1 (IPEC-1). In this infection model, 32 μg/mL Q4-15a-1 can completely inhibit ETEC adhesion onto IPEC-1. Overall, these results suggested that Q4-15a-1 may be a promising antibacterial candidate for treatment of weaned piglets infected by MDR ETEC.
وصف الملف: application/pdf
العلاقة: Molecular Pharmacology; https://dx.doi.org/10.3390/ijms22083926Test
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2دورية أكاديمية
المؤلفون: Hongbo Liu, Binghua Zhu, Beibei Liang, Xuebin Xu, Shaofu Qiu, Leili Jia, Peng Li, Lang Yang, Yongrui Li, Ying Xiang, Jing Xie, Ligui Wang, Chaojie Yang, Yansong Sun, Hongbin Song
المصدر: Frontiers in Microbiology, Vol 9 (2018)
مصطلحات موضوعية: mobilized colistin resistance (mcr-1) gene, novel mcr-1 variant, clinical diarrhea, enterotoxigenic Escherichia coli (ETEC), multidrug resistance (MDR), Microbiology, QR1-502
الوصف: In this study, we discovered a novel mobilized colistin resistance (mcr-1) gene variant, named mcr-1.9, which was identified in a colistin-resistant enterotoxigenic Escherichia coli (ETEC) strain from a clinical diarrhea case. The mcr-1.9 gene differs from mcr-1 at position 1036 due to a single nucleotide polymorphism (G→A), which results in an aspartic acid residue being replaced by an asparagine residue (Asp346→Asn) in the MCR-1 protein sequence. Antimicrobial susceptibility testing showed that the mcr-1.9-harboring ETEC strain is resistant to colistin at a minimum inhibitory concentration of 4 μg/ml. Plasmid profiling and conjugation experiments also suggest that the mcr-1.9 variant can be successfully transferred into the E. coli strain J53, indicating that the gene is located on a transferable plasmid. Bioinformatics analysis of data obtained from genome sequencing indicates that the mcr-1.9 gene is located on a 64,005 bp plasmid which has been named pEC26. This plasmid was found to have high similarity to the mcr-1-bearing IncI2-type plasmids pWF-5-19C (99% identity and 99% coverage) and pmcr1-IncI2 (99% identity and 98% coverage). The mcr-1.9-harboring ETEC also shows multidrug resistance to nine classes of antibiotics, and contains several virulence and antimicrobial-resistance genes suggested by the genome sequence analysis. Our report is the first to identify a new mcr-1 variant in an ETEC isolated from a human fecal sample, raising concerns about the existence of more such variants in human intestinal flora. Therefore, we believe that an undertaking to identify new mcr-1 variants in the bacterial communities of human intestines is of utmost importance, and that measures need to be taken to control the spread of mcr-1 and its variants in human intestinal microflora.
وصف الملف: electronic resource
العلاقة: http://journal.frontiersin.org/article/10.3389/fmicb.2018.00815/fullTest; https://doaj.org/toc/1664-302XTest
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3
المؤلفون: Yao-Kuan Huang, Kang-Chi Wu, Hui-Wen Chang, Kuo-Feng Hua, Ting-Ting Cheng, Wei-Jung Chen, Yeong-Hsiang Cheng, Yu-Hsiang Yu
المصدر: International Journal of Molecular Sciences
Volume 22
Issue 8
International Journal of Molecular Sciences, Vol 22, Iss 3926, p 3926 (2021)مصطلحات موضوعية: 0301 basic medicine, Swine, Antibiotics, medicine.disease_cause, Bacterial Adhesion, lcsh:Chemistry, Enterotoxigenic Escherichia coli, Drug Resistance, Multiple, Bacterial, enterotoxigenic Escherichia coli (ETEC), lcsh:QH301-705.5, Spectroscopy, Cellular localization, Escherichia coli Infections, Swine Diseases, biology, Chemistry, General Medicine, Hemolysis, Computer Science Applications, antimicrobial peptides (AMPs), Anti-Bacterial Agents, Pore Forming Cytotoxic Proteins, medicine.drug_class, 030106 microbiology, Antimicrobial peptides, Microbial Sensitivity Tests, Article, Catalysis, Microbiology, Inorganic Chemistry, 03 medical and health sciences, medicine, Animals, Physical and Theoretical Chemistry, Molecular Biology, antibiofilm, Organic Chemistry, Biofilm, medicine.disease, biology.organism_classification, Multiple drug resistance, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, Biofilms, intestinal porcine epithelial cell-1 (IPEC-1), multidrug resistance (MDR), Bacteria
الوصف: Post-weaning diarrhea due to enterotoxigenic Escherichia coli (ETEC) is a common disease of piglets and causes great economic loss for the swine industry. Over the past few decades, decreasing effectiveness of conventional antibiotics has caused serious problems because of the growing emergence of multidrug-resistant (MDR) pathogens. Various studies have indicated that antimicrobial peptides (AMPs) have potential to serve as an alternative to antibiotics owing to rapid killing action and highly selective toxicity. Our previous studies have shown that AMP GW-Q4 and its derivatives possess effective antibacterial activities against the Gram-negative bacteria. Hence, in the current study, we evaluated the antibacterial efficacy of GW-Q4 and its derivatives against MDR ETEC and their minimal inhibition concentration (MIC) values were determined to be around 2~32 μg/mL. Among them, AMP Q4-15a-1 with the second lowest MIC (4 μg/mL) and the highest minimal hemolysis concentration (MHC, 256 μg/mL), thus showing the greatest selectivity (MHC/MIC = 64) was selected for further investigations. Moreover, Q4-15a-1 showed dose-dependent bactericidal activity against MDR ETEC in time–kill curve assays. According to the cellular localization and membrane integrity analyses using confocal microscopy, Q4-15a-1 can rapidly interact with the bacterial surface, disrupt the membrane and enter cytosol in less than 30 min. Minimum biofilm eradication concentration (MBEC) of Q4-15a-1 is 4× MIC (16 μg/mL), indicating that Q4-15a-1 is effective against MDR ETEC biofilm. Besides, we established an MDR ETEC infection model with intestinal porcine epithelial cell-1 (IPEC-1). In this infection model, 32 μg/mL Q4-15a-1 can completely inhibit ETEC adhesion onto IPEC-1. Overall, these results suggested that Q4-15a-1 may be a promising antibacterial candidate for treatment of weaned piglets infected by MDR ETEC.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ba0df74014eafe3b8d62a459499e663Test
https://pubmed.ncbi.nlm.nih.gov/33920239Test -
4
المؤلفون: Hongbo Liu, Binghua Zhu, Beibei Liang, Xuebin Xu, Shaofu Qiu, Leili Jia, Peng Li, Lang Yang, Yongrui Li, Ying Xiang, Jing Xie, Ligui Wang, Chaojie Yang, Yansong Sun, Hongbin Song
مصطلحات موضوعية: Microbiology, Microbial Genetics, Microbial Ecology, Mycology, mobilized colistin resistance (mcr-1) gene, novel mcr-1 variant, clinical diarrhea, enterotoxigenic Escherichia coli (ETEC), multidrug resistance (MDR)
الوصف: In this study, we discovered a novel mobilized colistin resistance (mcr-1) gene variant, named mcr-1.9, which was identified in a colistin-resistant enterotoxigenic Escherichia coli (ETEC) strain from a clinical diarrhea case. The mcr-1.9 gene differs from mcr-1 at position 1036 due to a single nucleotide polymorphism (G→A), which results in an aspartic acid residue being replaced by an asparagine residue (Asp346→Asn) in the MCR-1 protein sequence. Antimicrobial susceptibility testing showed that the mcr-1.9-harboring ETEC strain is resistant to colistin at a minimum inhibitory concentration of 4 μg/ml. Plasmid profiling and conjugation experiments also suggest that the mcr-1.9 variant can be successfully transferred into the E. coli strain J53, indicating that the gene is located on a transferable plasmid. Bioinformatics analysis of data obtained from genome sequencing indicates that the mcr-1.9 gene is located on a 64,005 bp plasmid which has been named pEC26. This plasmid was found to have high similarity to the mcr-1-bearing IncI2-type plasmids pWF-5-19C (99% identity and 99% coverage) and pmcr1-IncI2 (99% identity and 98% coverage). The mcr-1.9-harboring ETEC also shows multidrug resistance to nine classes of antibiotics, and contains several virulence and antimicrobial-resistance genes suggested by the genome sequence analysis. Our report is the first to identify a new mcr-1 variant in an ETEC isolated from a human fecal sample, raising concerns about the existence of more such variants in human intestinal flora. Therefore, we believe that an undertaking to identify new mcr-1 variants in the bacterial communities of human intestines is of utmost importance, and that measures need to be taken to control the spread of mcr-1 and its variants in human intestinal microflora.
الإتاحة: https://doi.org/10.3389/fmicb.2018.00815.s002Test
https://figshare.com/articles/dataset/Data_Sheet_2_A_Novel_mcr-1_Variant_Carried_by_an_IncI2-Type_Plasmid_Identified_From_a_Multidrug_Resistant_Enterotoxigenic_Escherichia_coli_ZIP/6180260Test -
5
المؤلفون: Hongbo Liu, Binghua Zhu, Beibei Liang, Xuebin Xu, Shaofu Qiu, Leili Jia, Peng Li, Lang Yang, Yongrui Li, Ying Xiang, Jing Xie, Ligui Wang, Chaojie Yang, Yansong Sun, Hongbin Song
مصطلحات موضوعية: Microbiology, Microbial Genetics, Microbial Ecology, Mycology, mobilized colistin resistance (mcr-1) gene, novel mcr-1 variant, clinical diarrhea, enterotoxigenic Escherichia coli (ETEC), multidrug resistance (MDR)
الوصف: In this study, we discovered a novel mobilized colistin resistance (mcr-1) gene variant, named mcr-1.9, which was identified in a colistin-resistant enterotoxigenic Escherichia coli (ETEC) strain from a clinical diarrhea case. The mcr-1.9 gene differs from mcr-1 at position 1036 due to a single nucleotide polymorphism (G→A), which results in an aspartic acid residue being replaced by an asparagine residue (Asp346→Asn) in the MCR-1 protein sequence. Antimicrobial susceptibility testing showed that the mcr-1.9-harboring ETEC strain is resistant to colistin at a minimum inhibitory concentration of 4 μg/ml. Plasmid profiling and conjugation experiments also suggest that the mcr-1.9 variant can be successfully transferred into the E. coli strain J53, indicating that the gene is located on a transferable plasmid. Bioinformatics analysis of data obtained from genome sequencing indicates that the mcr-1.9 gene is located on a 64,005 bp plasmid which has been named pEC26. This plasmid was found to have high similarity to the mcr-1-bearing IncI2-type plasmids pWF-5-19C (99% identity and 99% coverage) and pmcr1-IncI2 (99% identity and 98% coverage). The mcr-1.9-harboring ETEC also shows multidrug resistance to nine classes of antibiotics, and contains several virulence and antimicrobial-resistance genes suggested by the genome sequence analysis. Our report is the first to identify a new mcr-1 variant in an ETEC isolated from a human fecal sample, raising concerns about the existence of more such variants in human intestinal flora. Therefore, we believe that an undertaking to identify new mcr-1 variants in the bacterial communities of human intestines is of utmost importance, and that measures need to be taken to control the spread of mcr-1 and its variants in human intestinal microflora.
الإتاحة: https://doi.org/10.3389/fmicb.2018.00815.s005Test
https://figshare.com/articles/dataset/Table_1_A_Novel_mcr-1_Variant_Carried_by_an_IncI2-Type_Plasmid_Identified_From_a_Multidrug_Resistant_Enterotoxigenic_Escherichia_coli_XLSX/6180269Test -
6صورة
المؤلفون: Hongbo Liu, Binghua Zhu, Beibei Liang, Xuebin Xu, Shaofu Qiu, Leili Jia, Peng Li, Lang Yang, Yongrui Li, Ying Xiang, Jing Xie, Ligui Wang, Chaojie Yang, Yansong Sun, Hongbin Song
مصطلحات موضوعية: Microbiology, Microbial Genetics, Microbial Ecology, Mycology, mobilized colistin resistance (mcr-1) gene, novel mcr-1 variant, clinical diarrhea, enterotoxigenic Escherichia coli (ETEC), multidrug resistance (MDR)
الوصف: In this study, we discovered a novel mobilized colistin resistance (mcr-1) gene variant, named mcr-1.9, which was identified in a colistin-resistant enterotoxigenic Escherichia coli (ETEC) strain from a clinical diarrhea case. The mcr-1.9 gene differs from mcr-1 at position 1036 due to a single nucleotide polymorphism (G→A), which results in an aspartic acid residue being replaced by an asparagine residue (Asp346→Asn) in the MCR-1 protein sequence. Antimicrobial susceptibility testing showed that the mcr-1.9-harboring ETEC strain is resistant to colistin at a minimum inhibitory concentration of 4 μg/ml. Plasmid profiling and conjugation experiments also suggest that the mcr-1.9 variant can be successfully transferred into the E. coli strain J53, indicating that the gene is located on a transferable plasmid. Bioinformatics analysis of data obtained from genome sequencing indicates that the mcr-1.9 gene is located on a 64,005 bp plasmid which has been named pEC26. This plasmid was found to have high similarity to the mcr-1-bearing IncI2-type plasmids pWF-5-19C (99% identity and 99% coverage) and pmcr1-IncI2 (99% identity and 98% coverage). The mcr-1.9-harboring ETEC also shows multidrug resistance to nine classes of antibiotics, and contains several virulence and antimicrobial-resistance genes suggested by the genome sequence analysis. Our report is the first to identify a new mcr-1 variant in an ETEC isolated from a human fecal sample, raising concerns about the existence of more such variants in human intestinal flora. Therefore, we believe that an undertaking to identify new mcr-1 variants in the bacterial communities of human intestines is of utmost importance, and that measures need to be taken to control the spread of mcr-1 and its variants in human intestinal microflora.
الإتاحة: https://doi.org/10.3389/fmicb.2018.00815.s004Test
https://figshare.com/articles/figure/Image_2_A_Novel_mcr-1_Variant_Carried_by_an_IncI2-Type_Plasmid_Identified_From_a_Multidrug_Resistant_Enterotoxigenic_Escherichia_coli_JPEG/6180266Test
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