دورية أكاديمية
A conserved megaprotein-based molecular bridge critical for lipid trafficking and cold resilience
| العنوان: | A conserved megaprotein-based molecular bridge critical for lipid trafficking and cold resilience |
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| المؤلفون: | Wang, Changnan, Wang, Bingying, Pandey, Taruna, Long, Yong, Zhang, Jianxiu, Oh, Fiona, Sima, Jessica, Guo, Ruyin, Liu, Yun, Zhang, Chao, Mukherjee, Shaeri, Bassik, Michael, Lin, Weichun, Deng, Huichao, Vale, Goncalo, McDonald, Jeffrey G., Shen, Kang, Ma, Dengke K. |
| المساهمون: | U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences, David and Lucile Packard Foundation |
| المصدر: | Nature Communications ; volume 13, issue 1 ; ISSN 2041-1723 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | General Physics and Astronomy, General Biochemistry, Genetics and Molecular Biology, General Chemistry, Multidisciplinary |
| الوصف: | Cells adapt to cold by increasing levels of unsaturated phospholipids and membrane fluidity through conserved homeostatic mechanisms. Here we report an exceptionally large and evolutionarily conserved protein LPD-3 in C. elegans that mediates lipid trafficking to confer cold resilience. We identify lpd-3 mutants in a mutagenesis screen for genetic suppressors of the lipid desaturase FAT-7. LPD-3 bridges the endoplasmic reticulum (ER) and plasma membranes (PM), forming a structurally predicted hydrophobic tunnel for lipid trafficking. lpd-3 mutants exhibit abnormal phospholipid distribution, diminished FAT-7 abundance, organismic vulnerability to cold, and are rescued by Lecithin comprising unsaturated phospholipids. Deficient lpd-3 homologues in Zebrafish and mammalian cells cause defects similar to those observed in C. elegans . As mutations in BLTP1 , the human orthologue of lpd-3 , cause Alkuraya-Kucinskas syndrome, LPD-3 family proteins may serve as evolutionarily conserved highway bridges critical for ER-associated non-vesicular lipid trafficking and resilience to cold stress in eukaryotic cells. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41467-022-34450-y |
| الإتاحة: | https://doi.org/10.1038/s41467-022-34450-yTest https://www.nature.com/articles/s41467-022-34450-y.pdfTest https://www.nature.com/articles/s41467-022-34450-yTest |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.FED081DF |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-022-34450-y |
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