دورية أكاديمية
Rheology predicts sputum eosinophilia in patients with muco-obstructive lung diseases
| العنوان: | Rheology predicts sputum eosinophilia in patients with muco-obstructive lung diseases |
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| المؤلفون: | Volpato, Mathilde, Vialaret, Jérôme, Hirtz, Christophe, Petit, Aurélie, Suehs, Carey, Patarin, Jérémy, Matzner-Lober, Eric, Vachier, Isabelle, Molinari, Nicolas, Bourdin, Arnaud, Charriot, Jérémy |
| المساهمون: | Université de Montpellier (UM), Centre Hospitalier Régional Universitaire Montpellier (CHRU Montpellier), Plateforme de Protéomique Clinique de Montpellier (PPC), BioCampus (BCM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Centre Hospitalier Régional Universitaire Montpellier (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Institut des Neurosciences de Montpellier (INM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Université de Rennes 2 (UR2), Hôpital de la Colombière, Institut Montpelliérain Alexander Grothendieck (IMAG), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Médecine de précision par intégration de données et inférence causale (PREMEDICAL), Inria Sophia Antipolis - Méditerranée (CRISAM), Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Institut Desbrest de santé publique (IDESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Physiologie & médecine expérimentale du Cœur et des Muscles U 1046 (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) |
| المصدر: | ISSN: 0006-291X. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2022 |
| المجموعة: | Archive Ouverte de l'Université Rennes (HAL) |
| مصطلحات موضوعية: | Asthma, COPD, Eosinophil, Mucins, Non-CF bronchiectasis, MESH: Asthma, MESH: Cross-Sectional Studies, MESH: Eosinophilia, MESH: Humans, MESH: Prospective Studies, MESH: Pulmonary Disease, Chronic Obstructive, MESH: Rheology, MESH: Sputum, [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph], [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract |
| الوصف: | International audience ; Background: Mucus is known to play a pathogenic role in muco-obstructive lung diseases, but little is known about the determinants of mucus rheology. The purpose of this study is to determine which sputum components influence sputum rheology in patients with muco-obstructive lung diseases.Methods: We performed a cross sectional prospective cohort study. Spontaneous sputum was collected from consecutive patients with muco-obstructive lung diseases. Sputum rheology was assessed using the Rheomuco® rheometer (Rheonova, Grenoble); the elastic modulus G′, viscous modulus G″, and the critical stress threshold σc were recorded. Key quantitative and qualitative biological sputum components were determined by cytology, nucleic acid amplification tests and mass spectrometry.Results: 48 patients were included from January to August 2019. Among them, 10 had asthma, 14 COPD and 24 non-CF bronchiectasis (NCFB). The critical stress threshold σc predicted a sputum eosinophilia superior to 1.25% with 89.19% accuracy (AUC = 0.8762). G′ and G″ are positively correlated with MUC5AC protein concentration ((rho = 0.361; P = .013) and (rho = 0.335; P = .021), respectively). σc was positively correlated with sputum eosinophilia (rho = 0.394; P = .012), MUC5B (rho = 0.552; P < .001) and total protein (rho = 0.490; P < .001) concentrations. G′ and G″ were significantly higher in asthma patients (G’ = 14.49[7.18–25.26]Pa, G’‘ = 3.0[2.16–5.38]Pa) compared to COPD (G’ = 5.01[2.94–6.48]Pa, P = .010; G’‘ = 1.45[1.16–1.94]Pa, P = .006) and to NCFB (G’ = 4.99[1.49–10.49]Pa, P = .003; G’’ = 1.46[0.71–2.47]Pa, P = .002).Conclusion: In muco-obstructive lung diseases, rheology predicts sputum eosinophilia and is correlated with mucin concentrations, regardless of the underlying disease.Clinical trial registration: (registrar, website, and registration number), where applicable NCT04081740. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-00-878144-6 0-00-878144-3 |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/35843096; hal-03725444; https://hal.science/hal-03725444Test; https://hal.science/hal-03725444/documentTest; https://hal.science/hal-03725444/file/2022%20Volpato%20et%20al.,%20Rheology.pdfTest; PUBMED: 35843096; WOS: 000878144300010 |
| DOI: | 10.1016/j.bbrc.2022.07.025 |
| الإتاحة: | https://doi.org/10.1016/j.bbrc.2022.07.025Test https://hal.science/hal-03725444Test https://hal.science/hal-03725444/documentTest https://hal.science/hal-03725444/file/2022%20Volpato%20et%20al.,%20Rheology.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.7DCDB6DD |
| قاعدة البيانات: | BASE |
| ردمك: | 9780008781446 0008781443 |
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| DOI: | 10.1016/j.bbrc.2022.07.025 |