Motor Recovery and Synaptic Preservation after Ventral Root Avulsion and Repair with a Fibrin Sealant Derived from Snake Venom
| العنوان: | Motor Recovery and Synaptic Preservation after Ventral Root Avulsion and Repair with a Fibrin Sealant Derived from Snake Venom |
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| المؤلفون: | Roberta Barbizan, Alexandre Leite Rodrigues de Oliveira, Mateus Vidigal de Castro, Antonio de Castro Rodrigues, Rui Seabra Ferreira, Benedito Barraviera |
| المساهمون: | Universidade Estadual de Campinas (UNICAMP), Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp) |
| المصدر: | PLoS ONE PLoS ONE, Vol 8, Iss 5, p e63260 (2013) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| بيانات النشر: | Public Library of Science, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Central Nervous System, Anatomy and Physiology, synaptophysin, microglia, reimplantation, animal cell, Avulsion, synapse, Neurotrophic factors, rat, neurotrophic factor, nerve regeneration, Fibrin glue, Radiculopathy, motoneuron, snake venom, Motor Neurons, Multidisciplinary, quantitative analysis, Microfilament Proteins, Anatomy, Animal Models, unclassified drug, Up-Regulation, fibrin glue, female, medicine.anatomical_structure, Neuroprotective Agents, Spinal Cord, glial fibrillary acidic protein, motor performance, nerve surgery, Peripheral nervous system, immunohistochemistry, Medicine, Female, Avulsion injury, Spinal Nerve Roots, Immunohistochemical Analysis, Neuroglia, Research Article, Snake Venoms, Neural Networks, ventral root, Science, animal experiment, Central nervous system, Immunology, Synaptophysin, Down-Regulation, Fibrin Tissue Adhesive, Biology, cell survival, Fibrin, Neurological System, reverse transcription polymerase chain reaction, Prosthesis Implantation, avulsion injury, Model Organisms, Peripheral Nervous System, Glial Fibrillary Acidic Protein, medicine, Pressure, Animals, controlled study, Nerve Growth Factors, Motor Systems, Wound Healing, nonhuman, animal model, Calcium-Binding Proteins, nerve cell plasticity, Recovery of Function, Spinal cord, medicine.disease, microenvironment, Nerve Regeneration, Rats, VENENOS DE ORIGEM ANIMAL, protein Iba1, nervous system, Rats, Inbred Lew, Synapses, biology.protein, Immunologic Techniques, Rat, Neuroscience |
| الوصف: | Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:29:28Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:37:31Z : No. of bitstreams: 1 2-s2.0-84877134844.pdf: 3249728 bytes, checksum: edbcaf0e91901535af179bfd090c34ff (MD5) Made available in DSpace on 2014-05-27T11:29:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-05-07 Background:Ventral root avulsion is an experimental model of proximal axonal injury at the central/peripheral nervous system interface that results in paralysis and poor clinical outcome after restorative surgery. Root reimplantation may decrease neuronal degeneration in such cases. We describe the use of a snake venom-derived fibrin sealant during surgical reconnection of avulsed roots at the spinal cord surface. The present work investigates the effects of this fibrin sealant on functional recovery, neuronal survival, synaptic plasticity, and glial reaction in the spinal motoneuron microenvironment after ventral root reimplantation.Methodology/Principal Findings:Female Lewis rats (7 weeks old) were subjected to VRA and root replantation. The animals were divided into two groups: 1) avulsion only and 2) replanted roots with fibrin sealant derived from snake venom. Post-surgical motor performance was evaluated using the CatWalk system twice a week for 12 weeks. The rats were sacrificed 12 weeks after surgery, and their lumbar intumescences were processed for motoneuron counting and immunohistochemistry (GFAP, Iba-1 and synaptophysin antisera). Array based qRT-PCR was used to evaluate gene regulation of several neurotrophic factors and receptors as well as inflammatory related molecules. The results indicated that the root reimplantation with fibrin sealant enhanced motor recovery, preserved the synaptic covering of the motoneurons and improved neuronal survival. The replanted group did not show significant changes in microglial response compared to VRA-only. However, the astroglial reaction was significantly reduced in this group.Conclusions/Significance:In conclusion, the present data suggest that the repair of avulsed roots with snake venom fibrin glue at the exact point of detachment results in neuroprotection and preservation of the synaptic network at the microenvironment of the lesioned motoneurons. Also such procedure reduced the astroglial reaction and increased mRNA levels to neurotrophins and anti-inflammatory cytokines that may in turn, contribute to improving recovery of motor function. © 2013 Barbizan et al. Laboratory of Nerve Regeneration Department of Structural and Functional Biology University of Campinas - UNICAMP, Anatomy, Campinas FOB - USP, Bauru CEVAP - Unesp, Botucatu CEVAP - Unesp, Botucatu |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f299aeb7392c0fad26932d30d3c45a05Test http://europepmc.org/articles/PMC3646764Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f299aeb7392c0fad26932d30d3c45a05 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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