التفاصيل البيبلوغرافية
| العنوان: |
Molecular quality engineering for low cost vaccine production |
| المؤلفون: |
Routenberg Love, Kerry, Dalvie, Neil C., Christopher Love, J., Brady, Joseph R. |
| المصدر: |
Vaccine Technology VII |
| بيانات النشر: |
ECI Digital Archives |
| سنة النشر: |
2018 |
| المجموعة: |
Engineering Conferences International: ECI Digital Archives |
| مصطلحات موضوعية: |
Molecular engineering, product quality, functional genomics, transcriptomics, Pichia pastoris, Engineering |
| الوصف: |
Vaccines based on recombinant proteins provide a compelling case for low cost products with broad global accessibility. Protein immunogens are typically derived directly from native sequences found in bacterial or viral pathogens, and may not be well-suited for efficient expression in recombinant hosts. Native immunogens may also suffer from numerous challenges during expression that impact their quality or efficient production, including truncation, aggregation and poor stability. These challenges can lead to inefficiencies in manufacturing of subunit protein vaccines. Typically, recombinant vaccine manufacturing processes are complex, serial batch operations requiring extensive quality testing throughout to ensure product integrity. In response to the Gates Foundation’s Grand Challenge for Innovations in Vaccine Manufacturing for Global Markets, we are co-developing the ULTRA program for flexible, low cost vaccine products. This program aims to develop platform processes for production of recombinant vaccines. We believe that molecular design of the antigens provides a critical handle in improving antigen quality, manufacturability, and product stability, all of which could enable potent, low-cost vaccines. Addressing potential manufacturing challenges early on in product development should enable simple integrated processes for antigen production while minimizing costs associated with quality testing. To this end, we are demonstrating our platform approach with a recombinant trivalent subunit vaccine for rotavirus currently in clinical development. We chose to express the three VP8 subunits in Pichia pastoris to take advantage of the high titers of secreted proteins and minimal process-related contaminants typically experienced with this organism—critical features when developing simple intensified processes to meet our cost targets of $0.15/dose. Initial expression results showed the rotavirus antigens were poorly expressed and suffered from N-terminal truncation and aggregation—all of which were also ... |
| نوع الوثيقة: |
text |
| اللغة: |
unknown |
| العلاقة: |
https://dc.engconfintl.org/vt_vii/134Test |
| الإتاحة: |
https://dc.engconfintl.org/vt_vii/134Test |
| رقم الانضمام: |
edsbas.473D3466 |
| قاعدة البيانات: |
BASE |
