دورية أكاديمية
Bisphenol-S and Bisphenol-F alter mouse pancreatic β-cell ion channel expression and activity and insulin release through an estrogen receptor ERβ mediated pathway
| العنوان: | Bisphenol-S and Bisphenol-F alter mouse pancreatic β-cell ion channel expression and activity and insulin release through an estrogen receptor ERβ mediated pathway |
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| المؤلفون: | Marroquí, Laura, Martinez-Pinna, Juan, Castellano-Muñoz, Manuel, Dos Santos, Reinaldo S., Medina-Gali, Regla M., Soriano, Sergi, Quesada, Iván, Gustafsson, Jan-Ake, Encinar, José A., Nadal, Ángel |
| المساهمون: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología, Fisiología Neuroendocrina (FINE) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2021 |
| المجموعة: | RUA - Repositorio Institucional de la Universidad de Alicante |
| مصطلحات موضوعية: | Bisphenol, Islet of langerhans, Endocrine disrupting chemicals, Estrogen receptors, Molecular dynamics simulation, Fisiología |
| الوصف: | Bisphenol-S (BPS) and Bisphenol-F (BPF) are current Bisphenol-A (BPA) substitutes. Here we used pancreatic β-cells from wild type (WT) and estrogen receptor β (ERβ) knockout (BERKO) mice to investigate the effects of BPS and BPF on insulin secretion, and the expression and activity of ion channels involved in β-cell function. BPS or BPF rapidly increased insulin release and diminished ATP-sensitive K+ (KATP) channel activity. Similarly, 48 h treatment with BPS or BPF enhanced insulin release and decreased the expression of several ion channel subunits in β-cells from WT mice, yet no effects were observed in cells from BERKO mice. PaPE-1, a ligand designed to preferentially trigger extranuclear-initiated ER pathways, mimicked the effects of bisphenols, suggesting the involvement of extranuclear-initiated ERβ pathways. Molecular dynamics simulations indicated differences in ERβ ligand-binding domain dimer stabilization and solvation free energy among different bisphenols and PaPE-1. Our data suggest a mode of action involving ERβ whose activation alters three key cellular events in β-cell, namely ion channel expression and activity, and insulin release. These results may help to improve the hazard identification of bisphenols. ; This work was supported by BPU2017-86579-R (AN) and BFU2016-77125-R (IQ) and RTI2018-096724-B-C21 (JAE) supported by FEDER /Ministerio de Ciencia e Innovación-Agencia Estatal de Investigación, Spain. PROMETEO/2020/006 (AN), PROMETEO/2016/006 (JAE) and SEJI/2018/023 (LM) supported by Generalitat Valenciana, Spain. J-AG was supported by the Robert A. Welch Foundation (E 0004), USA. CIBERDEM is an initiative of the Instituto de Salud Carlos III, Spain. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.1016/j.chemosphere.2020.129051Test; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2017-86579-R; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2016-77125-R; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096724-B-C21; Chemosphere. 2021, 265: 129051. https://doi.org/10.1016/j.chemosphere.2020.129051Test; 0045-6535 (Print); 1879-1298 (Online); http://hdl.handle.net/10045/112363Test |
| DOI: | 10.1016/j.chemosphere.2020.129051 |
| الإتاحة: | https://doi.org/10.1016/j.chemosphere.2020.129051Test http://hdl.handle.net/10045/112363Test |
| حقوق: | © 2020 Elsevier Ltd. ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.D1EE67B9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.chemosphere.2020.129051 |
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