التفاصيل البيبلوغرافية
| العنوان: |
Single-Molecule Methods for Ligand Counting: Linking Ion Uptake to DNA Hairpin Folding. |
| المؤلفون: |
Dittmore, Andrew1, Landy, Jonathan1, Molzon, Adrian A.1, Saleh, Omar A.1 saleh@engineering.ucsb.edu |
| المصدر: |
Journal of the American Chemical Society. 4/23/2014, Vol. 136 Issue 16, p5974-5980. 7p. |
| مصطلحات موضوعية: |
*LIGANDS (Chemistry), *CONFORMATIONAL analysis, *SINGLE molecules, *MOLECULAR conformation, *HAIRPIN (Genetics), *DNA folding |
| مستخلص: |
Ligand associations play a significant role in biochemical processes, typically through stabilizing a particular conformation of a folded biomolecule. Here, we demonstrate the ability to measure the changes in the number of ligands associated with a single, stretched biomolecule as it undergoes a conformational change. We do this by combining thermodynamic theory with single-molecule measurements that directly track biomolecular conformation. We utilize this technique to determine the changes in the ionic atmosphere of a DNA hairpin undergoing a force-destabilized folding transition. We find that the number of counterions liberated upon DNA unfolding is a nonmonotonic function of the monovalent salt concentration of the solution, contrary to predictions from common nucleic acid models. This demonstrates that previously unobserved phenomena can be measured with our ligand counting approach. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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