المؤلفون: Chiliza, Zinhle Edith, Martínez-Oyanedel, José, Syed, Khajamohiddin

المساهمون: National Research Foundation

المصدر: Biophysical Reviews ; volume 12, issue 5, page 1217-1222 ; ISSN 1867-2450 1867-2469

مصطلحات موضوعية: Molecular Biology, Structural Biology, Biophysics

الإتاحة: https://doi.org/10.1007/s12551-020-00749-7Test

المؤلفون: Matowane, Retshedisitswe Godfrey, Wieteska, Lukasz, Bamal, Hans Denis, Kgosiemang, Ipeleng Kopano Rosinah, Van Wyk, Mari, Manume, Nessie Agnes, Abdalla, Sara Mohamed Hasaan, Mashele, Samson Sitheni, Gront, Dominik, Syed, Khajamohiddin

المساهمون: Technology and Innovation Agency, Research and Innovation Fund, National Centre for Research and Development

المصدر: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ; volume 1866, issue 1, page 166-177 ; ISSN 1570-9639

مصطلحات موضوعية: Molecular Biology, Biochemistry, Biophysics, Analytical Chemistry

الإتاحة: https://doi.org/10.1016/j.bbapap.2017.10.003Test
https://api.elsevier.com/content/article/PII:S1570963917302418?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1570963917302418?httpAccept=text/plainTest

المؤلفون: Pagadala, Nataraj S., Syed, Khajamohiddin, Tuszynski, Jack

المصدر: Biophysical Reviews ; volume 9, issue 2, page 91-102 ; ISSN 1867-2450 1867-2469

مصطلحات موضوعية: Molecular Biology, Structural Biology, Biophysics

الإتاحة: https://doi.org/10.1007/s12551-016-0247-1Test

المؤلفون: Pagadala, Nataraj S., Bjorndahl, Trent C., Joyce, Michael, Wishart, David S., Syed, Khajamohiddin, Landi, Abdolamir

المصدر: Bioorganic & Medicinal Chemistry ; volume 25, issue 20, page 5875-5888 ; ISSN 0968-0896

مصطلحات موضوعية: Organic Chemistry, Clinical Biochemistry, Drug Discovery, Pharmaceutical Science, Molecular Biology, Molecular Medicine, Biochemistry

الإتاحة: https://doi.org/10.1016/j.bmc.2017.09.024Test
https://api.elsevier.com/content/article/PII:S0968089617313299?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0968089617313299?httpAccept=text/plainTest

المؤلفون: Pakala Suresh Babu, Syed Khajamohiddin, Deviprasanna Chakka, Mike Merrick, Anirban Bhaduri, Ramanathan Sowdhamini, Dayananda Siddavattam

المصدر: Biochemical and Biophysical Research Communications. 351:675-681

مصطلحات موضوعية: Models, Molecular, Hydrolases, Protein Conformation, Molecular Sequence Data, Biophysics, Flavobacterium, Biochemistry, Catalysis, Hydrolase, Gene cluster, Catalytic triad, Computer Simulation, Amino Acid Sequence, Homology modeling, Molecular Biology, Binding Sites, biology, Structural gene, Active site, Cell Biology, biology.organism_classification, Enzyme Activation, Models, Chemical, biology.protein, Protein Binding, Homotetramer

الوصف: The organophosphate degrading (opd) gene cluster of plasmid pPDL2 of Flavobacterium sp. ATCC27551 contains a novel open-reading frame, orf243. This was predicted to encode an alpha/beta hydrolase distantly related to the meta-fission product (MFP) hydrolases such as XylF, PhnD, and CumD. By homology modeling Orf243 has most of the structural features of MFP hydrolases including the characteristic active site catalytic triad. The purified protein (designated MfhA) is a homotetramer and shows similar affinity for 2-hydroxy-6-oxohepta-2,4-dienoate (HOHD), 2-hydroxymuconic semialdehyde (HMSA), and 2-hydroxy-5-methylmuconic semialdehyde (HMMSA), the meta-fission products of 3-methyl catechol, catechol, and 4-methyl catechol. The unique catalytic properties of MfhA and the presence near its structural gene of cis-elements required for transposition suggest that mfhA has evolved towards encoding a common hydrolase that can act on meta-fission products containing either aldehyde or ketone groups.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9565f0450bd5fcfa1312b98164fe31a3Test
https://doi.org/10.1016/j.bbrc.2006.10.080Test

المؤلفون: Syed, Khajamohiddin, Shale, Karabo, Nazir, K. H. M. Nazmul Hussain, Krasevec, Nada, Mashele, Samson Sitheni, Pagadala, Nataraj Sekhar

المصدر: Genes & Genomics ; volume 36, issue 3, page 321-333 ; ISSN 1976-9571 2092-9293

مصطلحات موضوعية: Genetics, Molecular Biology, Biochemistry

الإتاحة: https://doi.org/10.1007/s13258-013-0170-9Test

المؤلفون: Mike Merrick, Syed Khajamohiddin, Dayananda Siddavattam, Bramanandam Manavathi, Suresh B. Pakala

المصدر: Applied and Environmental Microbiology. 69:2533-2539

مصطلحات موضوعية: DNA, Bacterial, Transposable element, Insecticides, Tn3 transposon, Molecular Sequence Data, Genetics and Molecular Biology, Biology, Flavobacterium, Applied Microbiology and Biotechnology, Nitrophenols, Open Reading Frames, Complete sequence, Organophosphorus Compounds, Plasmid, Pseudomonas, Gene cluster, Cloning, Molecular, Insertion sequence, Gene, Soil Microbiology, Transposase, Genetics, Base Sequence, Ecology, Chromosome Mapping, Molecular biology, Biodegradation, Environmental, Genes, Bacterial, Multigene Family, DNA Transposable Elements, Plasmids, Food Science, Biotechnology

الوصف: Several bacterial strains that can use organophosphate pesticides as a source of carbon have been isolated from soil samples collected from diverse geographical regions. All these organisms synthesize an enzyme called parathion hydrolase, and in each case the enzyme is encoded by a gene ( opd ) located on a large indigenous plasmid. These plasmids show considerable genetic diversity, but the region containing the opd gene is highly conserved. Two opd plasmids, pPDL2 from Flavobacterium sp. and pCMS1 from Pseudomonas diminuta , are well characterized, and in each of them a region of about 5.1 kb containing the opd gene shows an identical restriction pattern. We now report the complete sequence of the conserved region of plasmid pPDL2. The opd gene is flanked upstream by an insertion sequence, ISFlsp 1 , that is a member of the IS 21 family, and downstream by a Tn 3 -like element encoding a transposase and a resolvase. Adjacent to opd but transcribed in the opposite direction is an open reading frame ( orf243 ) with the potential to encode an aromatic hydrolase somewhat similar to Pseudomonas putida TodF. We have shown that orf243 encodes a polypeptide of 27 kDa, which plays a role in the degradation of p -nitrophenol and is likely to act in concert with opd in the degradation of parathion. The linkage of opd and orf243 , the organization of the genes flanking opd , and the wide geographical distribution of these genes suggest that this DNA sequence may constitute a complex catabolic transposon.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27c3a13256f30f8b40d466f6670f0fcfTest
https://doi.org/10.1128/aem.69.5.2533-2539.2003Test

المؤلفون: Syed, Khajamohiddin, Kattamuri, Chandramohan, Thompson, Thomas B., Yadav, Jagjit S.

المصدر: Archives of Biochemistry and Biophysics ; volume 509, issue 1, page 26-32 ; ISSN 0003-9861

مصطلحات موضوعية: Molecular Biology, Biochemistry, Biophysics

الإتاحة: https://doi.org/10.1016/j.abb.2011.02.023Test
https://api.elsevier.com/content/article/PII:S0003986111000877?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0003986111000877?httpAccept=text/plainTest

المؤلفون: Syed, Khajamohiddin, Doddapaneni, Harshavardhan, Subramanian, Venkataramanan, Lam, Ying Wai, Yadav, Jagjit S.

المساهمون: National Institutes of Health, National Institute of Environmental Health Sciences

المصدر: Biochemical and Biophysical Research Communications ; volume 399, issue 4, page 492-497 ; ISSN 0006-291X

مصطلحات موضوعية: Cell Biology, Molecular Biology, Biochemistry, Biophysics

الإتاحة: https://doi.org/10.1016/j.bbrc.2010.07.094Test
https://api.elsevier.com/content/article/PII:S0006291X10014142?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0006291X10014142?httpAccept=text/plainTest