المؤلفون: Tzagiollari, Antzela, Redmond, John, McCarthy, Helen O., Levingstone, Tanya J., Dunne, Nicholas J.

المصدر: Tzagiollari , A , Redmond , J , McCarthy , H O , Levingstone , T J & Dunne , N J 2024 , ' Multi-objective property optimisation of a phosphoserine-modified calcium phosphate cement for orthopaedic and dental applications using design of experiments methodology ' , Acta Biomaterialia , vol. 174 , pp. 447-462 . https://doi.org/10.1016/j.actbio.2023.11.024Test

مصطلحات موضوعية: Amino acids, Bioinspired adhesives, Bone stabilisation, Fracture repair, Phosphoserine, /dk/atira/pure/subjectarea/asjc/1300/1305, Biotechnology, /dk/atira/pure/subjectarea/asjc/2500/2502, Biomaterials, /dk/atira/pure/subjectarea/asjc/1300/1303, Biochemistry, /dk/atira/pure/subjectarea/asjc/2200/2204, Biomedical Engineering, /dk/atira/pure/subjectarea/asjc/1300/1312, Molecular Biology

الوصف: Phosphoserine is a ubiquitous molecule found in numerous proteins and, when combined with alpha-tricalcium phosphate (α-TCP) powder, demonstrates the ability to generate an adhesive biomaterial capable of stabilising and repairing bone fractures. Design of Experiments (DoE) approach was able to optimise the composition of phosphoserine-modified calcium phosphate cement (PM-CPC) demonstrating that the liquid:powder ratio (LPR) and quantity of phosphoserine (wt%) significantly influenced the handling, mechanical, and adhesion properties. Subsequently, the DoE optimisation process identified the optimal PM-CPC formulation, exhibiting a compressive strength of 29.2 ± 4.9 MPa and bond/shear strength of 3.6 ± 0.9 MPa after a 24 h setting reaction. Moreover, the optimal PM-CPC composition necessitated a mixing time of 20 s and displayed an initial setting time between 3 and 4 min, thus enabling homogenous mixing and precise delivery within a surgical environment. Notably, the PM-CPC demonstrated a bone-to-bone bond strength of 1.05 ± 0.3 MPa under wet conditions, coupled with a slow degradation rate during the first five days. These findings highlight the ability of PM-CPC to effectively support and stabilise bone fragments during the initial stages of natural bone healing. The developed PM-CPC formulations fulfil the clinical requirements for working and setting times, static mechanical, degradation properties, and injectability, enabling surgeons to stabilise complex bone fractures. This innovative bioinspired adhesive represents a significant advancement in the treatment of challenging bone injuries, offering precise delivery within a surgical environment and the potential to enhance patient outcomes.

وصف الملف: application/pdf

الإتاحة: https://doi.org/10.1016/j.actbio.2023.11.024Test
https://pure.qub.ac.uk/en/publications/64661e3f-0495-4ba1-9f9a-0092e86a6ec4Test
https://pureadmin.qub.ac.uk/ws/files/554664573/1-s2.0-S1742706123006803-main.pdfTest

المؤلفون: Rodgers, Aoife M., Lindsay, Jaime, Monahan, Avril, Dubois, Alice V., Faniyi, Aduragbemi A., Plant, Barry J., Mall, Marcus A., Ekkelenkamp, Miquel B., Elborn, Stuart, Ingram, Rebecca J.

المصدر: Rodgers , A M , Lindsay , J , Monahan , A , Dubois , A V , Faniyi , A A , Plant , B J , Mall , M A , Ekkelenkamp , M B , Elborn , S & Ingram , R J 2023 , ' Biologically relevant murine models of chronic Pseudomonas aeruginosa respiratory infection ' , Pathogens , vol. 12 , no. 8 , 1053 . https://doi.org/10.3390/pathogens12081053Test

مصطلحات موضوعية: General Immunology and Microbiology, Immunology and Allergy, Infectious Diseases, Microbiology (medical), Molecular Biology, /dk/atira/pure/subjectarea/asjc/2700/2723, name=Immunology and Allergy, /dk/atira/pure/subjectarea/asjc/1300/1312, name=Molecular Biology, /dk/atira/pure/subjectarea/asjc/2400/2400, name=General Immunology and Microbiology, /dk/atira/pure/subjectarea/asjc/2700/2726, name=Microbiology (medical), /dk/atira/pure/subjectarea/asjc/2700/2725, name=Infectious Diseases, /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being, name=SDG 3 - Good Health and Well-being

الوصف: Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen and the leading cause of infection in patients with cystic fibrosis (CF). The ability of P. aeruginosa to evade host responses and develop into chronic infection causes significant morbidity and mortality. Several mouse models have been developed to study chronic respiratory infections induced by P. aeruginosa, with the bead agar model being the most widely used. However, this model has several limitations, including the requirement for surgical procedures and high mortality rates. Herein, we describe novel and adapted biologically relevant models of chronic lung infection caused by P. aeruginosa. Three methods are described: a clinical isolate infection model, utilising isolates obtained from patients with CF; an incomplete antibiotic clearance model, leading to bacterial bounce-back; and the establishment of chronic infection; and an adapted water bottle chronic infection model. These models circumvent the requirement for a surgical procedure and, importantly, can be induced with clinical isolates of P. aeruginosa and in wild-type mice. We also demonstrate successful induction of chronic infection in the transgenic βENaC murine model of CF. We envisage that the models described will facilitate the investigations of host and microbial factors, and the efficacy of novel antimicrobials, during chronic P. aeruginosa respiratory infections.

وصف الملف: application/pdf

العلاقة: https://pure.qub.ac.uk/en/publications/c4e40b53-a2df-482c-86a4-27e5c46bc84dTest

الإتاحة: https://doi.org/10.3390/pathogens12081053Test
https://pure.qub.ac.uk/en/publications/c4e40b53-a2df-482c-86a4-27e5c46bc84dTest
https://pureadmin.qub.ac.uk/ws/files/505383992/pathogens_12_01053.pdfTest

المؤلفون: Jahami, Ali, Mouzanar, Hussein, Mahfouz, Sarah, Khatib, Jamal, Sonebi, Mohammed

المصدر: Jahami , A , Mouzanar , H , Mahfouz , S , Khatib , J & Sonebi , M 2023 , ' Effect of graphene as additive on the mechanical properties of concrete ' , Nanoworld Journal , vol. 9 , no. Special Issue 2 , pp. S87-S92 . https://doi.org/10.17756/nwj.2023-s2-015Test

مصطلحات موضوعية: Additive, Compressive strength, Concrete, Graphene, Mechanical properties, Sustainability, /dk/atira/pure/subjectarea/asjc/1300/1304, Biophysics, /dk/atira/pure/subjectarea/asjc/2500/2502, Biomaterials, /dk/atira/pure/subjectarea/asjc/2200/2204, Biomedical Engineering, /dk/atira/pure/subjectarea/asjc/1300/1312, Molecular Biology, /dk/atira/pure/subjectarea/asjc/2500/2507, Polymers and Plastics, /dk/atira/pure/subjectarea/asjc/1300/1306, Cancer Research, /dk/atira/pure/subjectarea/asjc/2300/2307, Health, Toxicology and Mutagenesis, /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being, SDG 3 - Good Health and Well-being

الوصف: Graphene is a smart and relatively new material that has promising applications in many engineering fields. Recently it was found that graphene can be produced from burning waste materials which will have an impact on cost and the wide applications in many engineering fields including construction. The addition of tiny amounts of graphene in concrete mixes can enhance the mechanical and durability properties. This research investigated the effect of graphene as additive on the mechanical properties of concrete. The addition percentage (by weight of cement) was 0.02%, 0.035%, and 0.05%. Cubic and cylindrical specimens were prepared to assess the compressive strength, flexural strength, and elasticity modulus of concrete. Results showed that adding graphene to concrete led to an increase in both compressive and flexural strength as well as the modulus of elasticity. This will help in reducing concrete dimensions in different structural members like columns and beams and will contribute towards sustainable development.

وصف الملف: application/pdf

الإتاحة: https://doi.org/10.17756/nwj.2023-s2-015Test
https://pure.qub.ac.uk/en/publications/db69f102-64a5-4088-9031-f4f8411a07e6Test
https://pureadmin.qub.ac.uk/ws/files/528439333/nwj_s2_ali_jahami_2_.pdfTest

المؤلفون: Kerr, Bríanna N., Duffy, Daniel, McInerney, Caitríona E., Hutchinson, Ashton, Dabaja, Inaya, Bazzi, Rana, Roux, Stéphane, Prise, Kevin M., Butterworth, Karl T.

المصدر: Kerr , B N , Duffy , D , McInerney , C E , Hutchinson , A , Dabaja , I , Bazzi , R , Roux , S , Prise , K M & Butterworth , K T 2023 , ' Evaluation of radiosensitization and cytokine modulation by differentially PEGylated gold nanoparticles in glioblastoma cells ' , International Journal of Molecular Sciences , vol. 24 , no. 12 , 10032 . https://doi.org/10.3390/ijms241210032Test

مصطلحات موضوعية: cytokines, glioblastoma, gold nanoparticles, radiation, radiobiology, radiotherapy, /dk/atira/pure/subjectarea/asjc/1500/1503, Catalysis, /dk/atira/pure/subjectarea/asjc/1300/1312, Molecular Biology, /dk/atira/pure/subjectarea/asjc/1600/1607, Spectroscopy, /dk/atira/pure/subjectarea/asjc/1700/1706, Computer Science Applications, /dk/atira/pure/subjectarea/asjc/1600/1606, Physical and Theoretical Chemistry, /dk/atira/pure/subjectarea/asjc/1600/1605, Organic Chemistry, /dk/atira/pure/subjectarea/asjc/1600/1604, Inorganic Chemistry

الوصف: Glioblastoma (GBM) is known as the most aggressive type of malignant brain tumour, with an extremely poor prognosis of approximately 12 months following standard-of-care treatment with surgical resection, radiotherapy (RT), and temozolomide treatment. Novel RT-drug combinations are urgently needed to improve patient outcomes. Gold nanoparticles (GNPs) have demonstrated significant preclinical potential as radiosensitizers due to their unique physicochemical properties and their ability to pass the blood–brain barrier. The modification of GNP surface coatings with poly(ethylene) glycol (PEG) confers several therapeutic advantages including immune avoidance and improved cellular localisation. This study aimed to characterise both the radiosensitizing and immunomodulatory properties of differentially PEGylated GNPs in GBM cells in vitro. Two GBM cell lines were used, U-87 MG and U-251 MG. The radiobiological response was evaluated by clonogenic assay, immunofluorescent staining of 53BP1 foci, and flow cytometry. Changes in the cytokine expression levels were quantified by cytokine arrays. PEGylation improved the radiobiological efficacy, with double-strand break induction being identified as an underlying mechanism. PEGylated GNPs also caused the greatest boost in RT immunogenicity, with radiosensitization correlating with a greater upregulation of inflammatory cytokines. These findings demonstrate the radiosensitizing and immunostimulatory potential of ID11 and ID12 as candidates for RT-drug combination in future GBM preclinical investigations.

وصف الملف: application/pdf

الإتاحة: https://doi.org/10.3390/ijms241210032Test
https://pure.qub.ac.uk/en/publications/05a87581-eb16-4eea-868c-7e1925bf2374Test
https://pureadmin.qub.ac.uk/ws/files/492399131/ijms_24_10032_v3.pdfTest

المؤلفون: Nemesházi, Edina, Sramkó, Gábor, Laczkó, Levente, Balogh, Emese, Szatmári, Lajos, Vili, Nóra, Ujhegyi, Nikolett, Üveges, Bálint, Bókony, Veronika

المصدر: Nemesházi , E , Sramkó , G , Laczkó , L , Balogh , E , Szatmári , L , Vili , N , Ujhegyi , N , Üveges , B & Bókony , V 2022 , ' Novel genetic sex markers reveal unexpected lack of, and similar susceptibility to, sex reversal in free-living common toads in both natural and anthropogenic habitats ' , Molecular Ecology , vol. 00 . https://doi.org/10.1111/mec.16388Test

مصطلحات موضوعية: amphibians, feminization, human-induced environmental change, molecular sex markers, sex change, sex-chromosome identification, /dk/atira/pure/subjectarea/asjc/1300, Biochemistry, Genetics and Molecular Biology(all), /dk/atira/pure/subjectarea/asjc/1300/1311, Genetics, /dk/atira/pure/subjectarea/asjc/1300/1312, Molecular Biology, /dk/atira/pure/subjectarea/asjc/1100/1105, Ecology, Evolution, Behavior and Systematics, /dk/atira/pure/subjectarea/asjc/1100/1103, Animal Science and Zoology

الوصف: Anthropogenic environmental changes are affecting biodiversity and microevolution worldwide. Ectothermic vertebrates are especially vulnerable because environmental changes can disrupt their sexual development and cause sex reversal, a mismatch between genetic and phenotypic sex. This can potentially lead to sex‐ratio distortion and population decline. Despite these implications, there is scarce empirical knowledge on the incidence of sex reversal in nature. Populations in anthropogenic environments may be exposed to sex‐reversing stimuli more frequently, which may lead to higher sex‐reversal rate or, alternatively, these populations may adapt to resist sex reversal. We developed PCR‐based genetic sex markers for the common toad (Bufo bufo) to assess the prevalence of sex reversal in wild populations living in natural, agricultural and urban habitats, and the susceptibility of the same populations to two ubiquitous oestrogenic pollutants in a common garden experiment. We found negligible sex‐reversal frequency in free‐living adults despite the presence of various endocrine‐disrupting pollutants in their breeding ponds. Individuals from different habitat types showed similar susceptibility to sex reversal in the laboratory: all genetic males developed female phenotype when exposed to 1 µg L−1 17α‐ethinylestradiol (EE2) during larval development, whereas no sex reversal occurred in response to 1 ng L−1 EE2 and a glyphosate‐based herbicide with 3 µg L−1 or 3 mg L−1 glyphosate. The latter results do not support that populations in anthropogenic habitats would have either increased propensity for or higher tolerance to chemically induced sex reversal. Thus, the extremely low sex‐reversal frequency in wild toads compared to other ectothermic vertebrates studied before might indicate idiosyncratic, potentially species‐specific resistance to sex reversal.

وصف الملف: application/pdf

الإتاحة: https://doi.org/10.1111/mec.16388Test
https://research.bangor.ac.uk/portal/en/researchoutputs/novel-genetic-sex-markers-reveal-unexpected-lack-of-and-similar-susceptibility-to-sex-reversal-in-freeliving-common-toads-in-both-natural-and-anthropogenic-habitatsTest(350ebbb9-dd83-4281-bb14-9ce222c9a700).html
https://research.bangor.ac.uk/ws/files/40857449/Molecular_Ecology_2022_Nemesh_zi_Novel_genetic_sex_markers_reveal_unexpected_lack_of_and_similar_susceptibility_to_1_.pdfTest

المؤلفون: Quinn, Gerard P., Sessler, Tamas, Ahmaderaghi, Baharak, Lambe, Shauna, VanSteenhouse, Harper, Lawler, Mark, Wappett, Mark, Seligmann, Bruce, Longley, Daniel B., McDade, Simon S.

المصدر: Quinn , G P , Sessler , T , Ahmaderaghi , B , Lambe , S , VanSteenhouse , H , Lawler , M , Wappett , M , Seligmann , B , Longley , D B & McDade , S S 2022 , ' classifieR a flexible interactive cloud-application for functional annotation of cancer transcriptomes ' , BMC Bioinformatics , vol. 23 , 114 . https://doi.org/10.1186/s12859-022-04641-xTest

مصطلحات موضوعية: Breast Neoplasms/genetics, Computational Biology/methods, Female, Gene Expression Profiling/methods, Humans, Software, Transcriptome, /dk/atira/pure/subjectarea/asjc/1300/1315, Structural Biology, /dk/atira/pure/subjectarea/asjc/1300/1303, Biochemistry, /dk/atira/pure/subjectarea/asjc/1300/1312, Molecular Biology, /dk/atira/pure/subjectarea/asjc/1700/1706, Computer Science Applications, /dk/atira/pure/subjectarea/asjc/2600/2604, Applied Mathematics, /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being, SDG 3 - Good Health and Well-being

الوصف: Background Transcriptionally informed predictions are increasingly important for sub-typing cancer patients, understanding underlying biology and to inform novel treatment strategies. For instance, colorectal cancers (CRCs) can be classified into four CRC consensus molecular subgroups (CMS) or five intrinsic (CRIS) sub-types that have prognostic and predictive value. Breast cancer (BRCA) has five PAM50 molecular subgroups with similar value, and the OncotypeDX test provides transcriptomic based clinically actionable treatment-risk stratification. However, assigning samples to these subtypes and other transcriptionally inferred predictions is time consuming and requires significant bioinformatics experience. There is no "universal" method of using data from diverse assay/sequencing platforms to provide subgroup classification using the established classifier sets of genes (CMS, CRIS, PAM50, OncotypeDX), nor one which in provides additional useful functional annotations such as cellular composition, single-sample Gene Set Enrichment Analysis, or prediction of transcription factor activity. Results To address this bottleneck, we developed classifieR, an easy-to-use R-Shiny based web application that supports flexible rapid single sample annotation of transcriptional profiles derived from cancer patient samples form diverse platforms. We demonstrate the utility of the " classifieR" framework to applications focused on the analysis of transcriptional profiles from colorectal (classifieRc) and breast (classifieRb). Samples are annotated with disease relevant transcriptional subgroups (CMS/CRIS sub-types in classifieRc and PAM50/inferred OncotypeDX in classifieRb), estimation of cellular composition using MCP-counter and xCell, single-sample Gene Set Enrichment Analysis (ssGSEA) and transcription factor activity predictions with Discriminant Regulon Expression Analysis (DoRothEA). Conclusions classifieR provides a framework which enables labs without access to a dedicated bioinformation can get information on the ...

وصف الملف: application/pdf

الإتاحة: https://doi.org/10.1186/s12859-022-04641-xTest
https://pure.qub.ac.uk/en/publications/3aca1733-7e40-4b75-9422-a771b9bc12e9Test
https://pureadmin.qub.ac.uk/ws/files/307548570/s12859_022_04641_x.pdfTest

المؤلفون: Wang, Shumei, Vetukuri, Ramesh R., Kushwaha, Sandeep K., Hedley, Pete E., Morris, Jenny, Studholme, David J., Welsh, Lydia R. J., Boevink, Petra C., Birch, Paul R. J., Whisson, Stephen C.

المصدر: Wang , S , Vetukuri , R R , Kushwaha , S K , Hedley , P E , Morris , J , Studholme , D J , Welsh , L R J , Boevink , P C , Birch , P R J & Whisson , S C 2021 , ' Haustorium formation and a distinct biotrophic transcriptome characterize infection of Nicotiana benthamiana by the tree pathogen Phytophthora kernoviae ' , Molecular Plant Pathology , vol. 22 , no. 8 , pp. 954-968 . https://doi.org/10.1111/mpp.13072Test

مصطلحات موضوعية: biotrophy, effector, pathogenicity, RNA‐, Seq, RXLR, tree disease, Plant Science, Soil Science, Molecular Biology, Agronomy and Crop Science, RNA-Seq, /dk/atira/pure/subjectarea/asjc/1300/1312, name=Molecular Biology, /dk/atira/pure/subjectarea/asjc/1100/1102, name=Agronomy and Crop Science, /dk/atira/pure/subjectarea/asjc/1100/1111, name=Soil Science, /dk/atira/pure/subjectarea/asjc/1100/1110, name=Plant Science

الوصف: Phytophthora species cause some of the most serious diseases of trees and threaten forests in many parts of the world. Despite the generation of genome sequence assemblies for over 10 tree-pathogenic Phytophthora species and improved detection methods, there are many gaps in our knowledge of how these pathogens interact with their hosts. To facilitate cell biology studies of the infection cycle we examined whether the tree pathogen Phytophthora kernoviae could infect the model plant Nicotiana benthamiana . We transformed P. kernoviae to express green fluorescent protein (GFP) and demonstrated that it forms haustoria within infected N. benthamiana cells. Haustoria were also formed in infected cells of natural hosts, Rhododendron ponticum and European beech ( Fagus sylvatica ). We analysed the transcriptome of P. kernoviae in cultured mycelia, spores, and during infection of N. benthamiana , and detected 12,559 transcripts. Of these, 1,052 were predicted to encode secreted proteins, some of which may function as effectors to facilitate disease development. From these, we identified 87 expressed candidate RXLR (Arg-any amino acid-Leu-Arg) effectors. We transiently expressed 12 of these as GFP fusions in N. benthamiana leaves and demonstrated that nine significantly enhanced P. kernoviae disease progression and diversely localized to the cytoplasm, nucleus, nucleolus, and plasma membrane. Our results show that N. benthamiana can be used as a model host plant for studying this tree pathogen, and that the interaction likely involves suppression of host immune responses by RXLR effectors. These results establish a platform to expand the understanding of Phytophthora tree diseases.

وصف الملف: application/pdf

العلاقة: https://discovery.dundee.ac.uk/en/publications/32ee6d1b-eef5-448e-8160-afadb83b357eTest

الإتاحة: https://doi.org/10.1111/mpp.13072Test
https://discovery.dundee.ac.uk/en/publications/32ee6d1b-eef5-448e-8160-afadb83b357eTest
https://discovery.dundee.ac.uk/ws/files/58767264/mpp.13072.pdfTest

المؤلفون: McMillan, Hayley, Lundy, Fionnuala T., Dunne, Orla M., Al-Natour, Banan, Jeanneau, Charlotte, About, Imad, Curtis, Tim M., El Karim, Ikhlas

المصدر: McMillan , H , Lundy , F T , Dunne , O M , Al-Natour , B , Jeanneau , C , About , I , Curtis , T M & El Karim , I 2021 , ' Endogenous Mas-related G-protein-coupled receptor X1 activates and sensitizes TRPA1 in a human model of peripheral nerves ' , The FASEB Journal , vol. 35 , no. 5 , e21492 . https://doi.org/10.1096/fj.202001667RRTest

مصطلحات موضوعية: dental pulp, human, MrgprX1, nociception, peripheral neurons, /dk/atira/pure/subjectarea/asjc/1300/1305, Biotechnology, /dk/atira/pure/subjectarea/asjc/1300/1303, Biochemistry, /dk/atira/pure/subjectarea/asjc/1300/1312, Molecular Biology, /dk/atira/pure/subjectarea/asjc/1300/1311, Genetics, /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being, SDG 3 - Good Health and Well-being

الوصف: Mas-related G-protein-coupled receptor X1 (MrgprX1) is a human-specific Mrgpr and its expression is restricted to primary sensory neurons. However, its role in nociception and pain signaling pathways is largely unknown. This study aims to investigate a role for MrgprX1 in nociception via interaction with the pain receptor, Transient Receptor Potential Ankyrin 1 (TRPA1), using in-vitro and in-vivo human neuronal models. MrgprX1 protein expression in human trigeminal nociceptors was investigated by the immunolabeling of the dental pulp and cultured peripheral neuronal equivalent (PNE) cells. MrgprX1 receptor signaling was monitored by Fura-2-based Ca2+ imaging using PNEs and membrane potential responses were measured using FluoVoltTM. Immunofluorescent staining revealed MrgprX1 expression in-vivo in dental afferents, which was more intense in inflamed compared to healthy dental pulps. Endogenous MrgprX1 protein expression was confirmed in the in-vitro human PNE model. MrgprX1 receptor signaling and the mechanisms through which it couples to TRPA1 were studied by Ca2+ imaging. Results showed that MrgprX1 activates TRPA1 and induces membrane depolarization in a TRPA1 dependent manner. In addition, MrgprX1 sensitizes TRPA1 to agonist stimulation via Protein Kinase C (PKC). The activation and sensitization of TRPA1 by MrgprX1 in a model of human nerves suggests an important role for this receptor in the modulation of nociception.

وصف الملف: application/pdf

الإتاحة: https://doi.org/10.1096/fj.202001667RRTest
https://pure.qub.ac.uk/en/publications/a6760323-fdd9-4204-a414-f1e7b60cb3e4Test
https://pureadmin.qub.ac.uk/ws/files/382041735/Human.pdfTest

المؤلفون: McMillan, Hayley, Lundy, Fionnuala T., Dunne, Orla M., Al-Natour, Banan, Jeanneau, Charlotte, About, Imad, Curtis, Tim M., El Karim, Ikhlas

المصدر: McMillan , H , Lundy , F T , Dunne , O M , Al-Natour , B , Jeanneau , C , About , I , Curtis , T M & El Karim , I 2021 , ' Endogenous Mas-related G-protein-coupled receptor X1 activates and sensitizes TRPA1 in a human model of peripheral nerves ' , The FASEB Journal , vol. 35 , no. 5 , e21492 , pp. e21492 . https://doi.org/10.1096/fj.202001667RRTest

مصطلحات موضوعية: dental pulp, human, MrgprX1, nociception, peripheral neurons, /dk/atira/pure/subjectarea/asjc/1300/1305, Biotechnology, /dk/atira/pure/subjectarea/asjc/1300/1303, Biochemistry, /dk/atira/pure/subjectarea/asjc/1300/1312, Molecular Biology, /dk/atira/pure/subjectarea/asjc/1300/1311, Genetics, /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being, SDG 3 - Good Health and Well-being

الوصف: Mas-related G-protein-coupled receptor X1 (MrgprX1) is a human-specific Mrgpr and its expression is restricted to primary sensory neurons. However, its role in nociception and pain signaling pathways is largely unknown. This study aims to investigate a role for MrgprX1 in nociception via interaction with the pain receptor, Transient Receptor Potential Ankyrin 1 (TRPA1), using in-vitro and in-vivo human neuronal models. MrgprX1 protein expression in human trigeminal nociceptors was investigated by the immunolabeling of the dental pulp and cultured peripheral neuronal equivalent (PNE) cells. MrgprX1 receptor signaling was monitored by Fura-2-based Ca2+ imaging using PNEs and membrane potential responses were measured using FluoVoltTM . Immunofluorescent staining revealed MrgprX1 expression in-vivo in dental afferents, which was more intense in inflamed compared to healthy dental pulps. Endogenous MrgprX1 protein expression was confirmed in the in-vitro human PNE model. MrgprX1 receptor signaling and the mechanisms through which it couples to TRPA1 were studied by Ca2+ imaging. Results showed that MrgprX1 activates TRPA1 and induces membrane depolarization in a TRPA1 dependent manner. In addition, MrgprX1 sensitizes TRPA1 to agonist stimulation via Protein Kinase C (PKC). The activation and sensitization of TRPA1 by MrgprX1 in a model of human nerves suggests an important role for this receptor in the modulation of nociception.

وصف الملف: application/pdf

الإتاحة: https://doi.org/10.1096/fj.202001667RRTest
https://pure.qub.ac.uk/en/publications/endogenous-masrelated-gproteincoupled-receptor-x1-activates-and-sensitizes-trpa1-in-a-human-model-of-peripheral-nervesTest(365926b1-5678-4d19-9a77-6bf16a3f89dd).html
https://pureadmin.qub.ac.uk/ws/files/237494988/Human.pdfTest

المؤلفون: Abuhammad, Areej, Falah, Jannat, Alfalah, Salsabeel F.M., Abu-Tarboush , Muhannad, Tarawneh , Ruba T., Drikakis, Dimitris, Charissis, Vassilis

المصدر: Abuhammad , A , Falah , J , Alfalah , S F M , Abu-Tarboush , M , Tarawneh , R T , Drikakis , D & Charissis , V 2021 , ' “MedChemVR”: a virtual reality game to enhance medicinal chemistry education ' , Multimodal Technologies and Interaction , vol. 5 , no. 3 , 10 . https://doi.org/10.3390/mti5030010Test

مصطلحات موضوعية: human-computer interaction, user experience, user interface design, medicinal chemistry, gamification, virtual reality, inclusive technology, e-learning, pharmacology, simulation, education, molecular biology, molecular dynamics, inclusive education, /dk/atira/pure/sustainabledevelopmentgoals/quality_education, name=SDG 4 - Quality Education, /dk/atira/pure/subjectarea/asjc/3300/3304, name=Education, /dk/atira/pure/subjectarea/asjc/2700/2736, name=Pharmacology (medical), /dk/atira/pure/subjectarea/asjc/1300/1312, name=Molecular Biology, /dk/atira/pure/subjectarea/asjc/1700/1709, name=Human-Computer Interaction, /dk/atira/pure/subjectarea/asjc/2200/2204, name=Biomedical Engineering, /dk/atira/pure/subjectarea/asjc/1700/1706, name=Computer Science Applications, /dk/atira/pure/subjectarea/asjc/1700/1704, name=Computer Graphics and Computer-Aided Design

الوصف: Medicinal chemistry (MC) is an indispensable component of the pharmacy curriculum. The pharmacists’ unique knowledge of a medicine’s chemistry enhances their understanding of the pharmacological activity, manufacturing, storage, use, supply, and handling of drugs. However, chemistry is a challenging subject for both teaching and learning. These challenges are typically caused by the inability of students to construct a mental image of the three-dimensional (3D) structure of a drug molecule from its two-dimensional presentations. This study explores a prototype virtual reality (VR) gamification option, as an educational tool developed to aid the learning process and to improve the delivery of the MC subject to students. The developed system is evaluated by a cohort of 41 students. The analysis of the results was encouraging and provided invaluable feedback for the future development of the proposed system.

وصف الملف: application/pdf

العلاقة: https://researchonline.gcu.ac.uk/en/publications/fb7f3636-5ece-45e7-a0cf-f309ac903539Test

الإتاحة: https://doi.org/10.3390/mti5030010Test
https://researchonline.gcu.ac.uk/en/publications/fb7f3636-5ece-45e7-a0cf-f309ac903539Test
https://researchonline.gcu.ac.uk/ws/files/43555258/Abuhammad_A._et_al_2021_MedChemVR_a_virtual_reality_game_to_enhance_medicinal_chemistry_education.pdfTest